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28 Nov 2006: 100 120. kDa 220. Nosto. c p. ly. sate. M. 0 0 0.2 0.4 0.6 0.8 1.0 1.2 40 80 60 20. ... 60. 40. 0. 20. 80. WT K82A mutant. 0.2 0.4 0.6 0.8 1.0 1.2.

13 Oct 2020: Catalogue Number (250 mL). 60% Precipitant Mix 1 40% v/v PEG 500 MME; 20 % w/v PEG 20000. ... MD2-100-82 MD2-250-82. 60% Precipitant Mix 3 40% v/v Glycerol; 20% w/v PEG 4000.

25 Jul 2007: 18:30 Dinner 20:00 Opening event: Concert at Schloß Waldthausen by „Trio con brio“ (piano trio) 22:00 Get together in the „Weinkeller“ of Schloß Waldthausen Thursday, Oct 11th, 2007

13 Dec 2017: Rinse the container of condition with deionized water and 20 % ethanol for reuse. ... 5. Seal the cell culture plate containing the screen with an aluminum sheet and place it back in the -20 C incubator.

19 Apr 2006: 2.2. Linear amplification reaction. 2–20 nM of PCR product was used in a linear amplification reaction with 6–400 pM ofpHis17 (B. ... The protein was purified over a Ni-NTA spin column under native conditions (lanes 3 and 5) and separated on a 10–20

1 Jul 2011: 2B). BtubA/B bilden in vitro dimere Filamente,die sich zu einem Komplex aus 20 bis 30 Fila-menten bündeln können.

3 Dec 2004: we solved the structureof the NH2-terminal domain from the closeAP180 homolog, CALM, at 2 Å resolution(19, 20) (crystals of AP180-N did not diffractwell). ... 20 November 2000; accepted 18 December 2000. Notch Inhibition of RASSignaling Through MAP

28 Sep 2006: The lysate buffer consisted of 50 mM Tris-Cl, 1.25 M sodium chloride, final pH 8.0, the wash buffer of 50 mM Tris-Cl, 500 mM sodium chloride, 20% ... For the phylogenetic tree, 10-20 sequences related to the different classes of dynamin-related proteins

21 Jun 2005: and size-exclusion chromatography (S300 Sephacryl,Amersham Pharmacia; 20 mM TrisHCl1 mM azide1 mMEDTA, pH 7.5). ... After 30 min at room temperature, samples werecentrifuged at 80,000 rpm for 20 min at 20C in a TLA100 rotor(Beckman).

6 Mar 2001: 0 10 20 300. 25. 50. 75. 100. Time (min). WT 500 µM GTP. ... Cellswere xed with 2% paraformaldehyde/1.5% glutaraldehyde in 0.1 M sodium cacodylatefor 20 min at room temperature.