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LMB Screen Scoring Sheet
https://www2.mrc-lmb.cam.ac.uk/groups/JYL/WWWrobots/LMB%20plates/LMB_21.pdf23 Sep 2016: G9 20% w/v PEG 8000, 0.2M magnesium acetate, 0.1M MES pH 6.5. ... G11 20% w/v PEG 8000, 0.2M magnesium chloride, 0.1M CAPS pH 9.0. -
Influence of lipid bilayer on the structure of the muscle-type…
https://www2.mrc-lmb.cam.ac.uk/groups/nu/pdf/pnasS24.pdf30 Apr 2024: Nat. Commun. 14, 3169 (2023). 20. P. Kumar et al., Cryo- EM structures of a lipid- sensitive pentameric ligand- gated ion channel embedded in a phosphatidylcholine- only bilayer. ... Electron Microscopy Data Bank https://www.ebi.ac.uk/emdb/EMD-18840. -
Morpheus Additive Screen Scoring Sheet MD1-93.xlsx Morpheus Additive…
https://www2.mrc-lmb.cam.ac.uk/groups/JYL/WWWrobots/LMB%20plates/Addit_01.pdf26 Nov 2015: A3 PEG 500 MME precipitant, 40% w/v. A4 PEG 8000 precipitant, 20% w/v. ... A5 Ethylene glycol cryoprotectant, 40% v/v. A6 PEG 4000 precipitant, 20% w/v. -
Expression of selenomethionine substituted proteins innon-methionine…
https://www2.mrc-lmb.cam.ac.uk/groups/JYL/methods/SeMet%20recipe.doc.pdf2 Feb 2004: Add reducing agents immediately before use. Minimal medium (per litre):1l M9 medium2ml 1M MgSO4 (2mM)20ml 20% glucose (0.4%)antibiotic(s)1ml vitamins 1000x (see below)10ml trace elements -
crib-sheet.dvi
https://www2.mrc-lmb.cam.ac.uk/personal/pemsley/coot/files/crib-sheet.pdf14 Oct 2016: i: Toggle continuous spin. 1.20 Baton Mode. b: toggle into baton rotate mode4. -
Alonsoetal Supplementary Material 030107
https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/Alonso-suppmat.pdf2 Apr 2007: Purified tubulins were desalted into PEM containing 20 μM GDP before storage in liquid nitrogen. ... Enzyme reactions were done in 20 mM PIPES pH 6.9, 2 mM MgCl2, using quartz fluorimeter cuvettes. -
HiroseSuppl
https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/supp_MC2006.pdf25 Aug 2006: The thickness of the. sections is 15 Å in (A-F), and 20 Å in (G-I). ... was reported to rotate clockwise by 20 in the ADP state compared with the AMPPNP state. -
DOI: 10.1126/science.1136985 , 120 (2007); 316Science et al.Maria C.…
https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/Alonso_Science.pdf6 Apr 2007: 0 5 10 15 20. 0 5 10 15 20. 0 5 10 15 20-10. ... Themicrotubule plus end is toward the top of thepage. (Left) Cryo-EM map (20). -
Endocytosis: Curvature proteins direct traffic
https://www2.mrc-lmb.cam.ac.uk/groups/hmm/publica/Papers/NRMCB_Fcho.pdf18 Jun 2010: 20 Macmillan Publishers Limited. All rights reserved10. -
Sup Fig 1
https://www2.mrc-lmb.cam.ac.uk/groups/hmm/publica/YopJSup.pdf11 Nov 2008: phospho IκB. IκB. YopJ-wt. TNF-α (min) 0 5 10 15 20 0 5 10 15 20. ... YopJ-C172A YopJ-wt. 0 5 10 15 20 0 5 10 15 20 TNF-α (min).
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