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https://www2.mrc-lmb.cam.ac.uk/groups/JYL/WWWrobots/LMB%20plates/Addit04b_AL-SEL-DP_Formatted.xlsx24 Nov 2020: C12E9. APO129? 0.05. 583. E. 11. 59. Polyoxyethylene(10)dodecyl ether. 20. C12E10. ... AP1210? 0.1. 627. E. 12. 60. Polyoxyethylene(8)tridecyl ether. 20. C13E8. APO138? -
31/10/2018 Carter lab TIRF microscopy Single Molecule Assay 5x ...
https://www2.mrc-lmb.cam.ac.uk/groups/cartera/download/resources/protocols/2020_Carter_TIRF_protocol.docx28 Jan 2020: 15 mg Glucose Oxidase 2mg Catalase (both in -20) into 250 μL DLB. ... Don’t pipette up and down. Could use a cut 20 μL tip for this. -
Carter lab insect cell ZZ-tag prep (2020) Buffers Wash ...
https://www2.mrc-lmb.cam.ac.uk/groups/cartera/download/resources/protocols/2020_Carter_Sf9_dynein_prep.docx24 Jan 2020: Gel-filtration on AKTAmicro. -Column is stored in water 0.05% NaN3. If stored in 20% ethanol, first wash into water before washing into buffer. ... Remove column and replace caps on either end. -Put inlet A1 into 20% ethanol, pumpwash. -
16001658465475 1..36
https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/Collier_2020_elife-59560-v1.pdf15 Sep 2020: reagent bis-maleimidoethane (BMOE). Around 20% of cohesin complexes can be crosslinked simul-. ... teins were denatured by adding SDS to a final concentration of 1% and heating at 70˚C for 20 min. -
Sheet1
https://www2.mrc-lmb.cam.ac.uk/groups/JYL/WWWrobots/LMB%20plates/Addit05_NeXtal%20pH%20gradient.xlsx8 Oct 2020: D1. 19. 5.8. tri-Sodium Citrate. 1. M. D2. 20. 6. Sodium Cacodylate. -
15955888524538 1..29
https://www2.mrc-lmb.cam.ac.uk/groups/hegde/wp-content/uploads/sites/8/2020/09/eLife_2020b.pdf24 Jul 2020: targeting siRNA and analysed by flow cytometry after 20 hr of reporter expression. ... These considerations suggest that a general detector of collisions must be within 20 fold abun-. -
An intramembrane chaperone complex facilitates membrane protein…
https://www2.mrc-lmb.cam.ac.uk/groups/hegde/wp-content/uploads/sites/8/2020/09/Nature_2020.pdf17 Aug 2020: A total of 20,000 GFP-positive (or RFP-positive for SQS and ASGR) cells were collected. ... The transcription reactions were con-ducted with 5–20 ng ml1 PCR product at 37 C. -
15919734904046 1..30
https://www2.mrc-lmb.cam.ac.uk/groups/hegde/wp-content/uploads/sites/8/2020/09/eLife_2020.pdf12 Jun 2020: CC1/2 (%) 100.0 (81.7) 100.0 (92.6). Refinement. Rwork/Rfree (%) - 20.3/25.0. ... Micrographs 2776 2484 1206 4228 932. Microscope tilt (degrees) 0 0 30 20 20.
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