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The structure of human thyroglobulin
https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/TG_Nature2020.pdf31 Jan 2020: J. Biol. Chem. 293, 4860–4869 (2018). 20. Yang, S.-X., Pollock, H. ... equili-brated with 20 mM Hepes, 200 mM NaCl at pH 7.5. -
MD1-118 Hippocrates additive screen
https://www2.mrc-lmb.cam.ac.uk/groups/JYL/WWWrobots/LMB%20plates/Addit-03.pdf13 Oct 2020: C1 13 9 % Pyridoxine hydrochloride C2 14 9 % Thiamine hydrochloride C3 15 9 % CytidineC4 16 6 % Inosine†C5 17 6 % RibavirinC6 18 6 % ThymidineD1 19 6 % UridineD2 20 1.5 % -
FtsK in motion reveals its mechanism for double-stranded DNA…
https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/FtsK_Jean_2020_PNAS.pdf8 Jun 2020: For thedsDNA, a 20-bp B-form dsDNA model was generated in Coot (32) and fittedmanually inside the map. ... Proc. Natl. Acad. Sci.U.S.A. 114, E697–E706 (2017). 20. A. H. de la Peña, E. -
EMBO Practical Course on the Crystallization of Macromolecular…
https://www2.mrc-lmb.cam.ac.uk/groups/cartera/download/cv/2020_01_Carter_CV.pdf31 Jan 2020: Nat Struct Mol Biol. 19: 492-7. 20) Carter AP‡, Cho C, Jin L, Vale RD‡ (2011) Crystal structure of the dynein motor. -
Carter lab dynactin prep from frozen porcine brains, using ...
https://www2.mrc-lmb.cam.ac.uk/groups/cartera/download/2020_Carter_dynactin_brain_prep.docx28 Feb 2020: Overnight, wash the G4000 from storage in 20% EtOH to water (2 CV). ... Stored in. SP-sepharose. 250. 0.3. 4. 20% EtOH. MonoQ 16/10. 20. -
Identifying proteins bound to native mitotic ESC chromosomes reveals…
https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/Djeghloul%20Nat%20Camms%202020.pdf11 Aug 2020: 40 50. Chromomycin A3. Chromosome 19: Genomic position. 10,000,000 20,000,000 30,000,000 40,000,000 50,000,000 60,000,000. ... μm. 2 )C. entr. omer. e si. ze (. μm2 ). 0. 10. 20. 30. 40. 50. 40. 30. 20 30 40 20 30 40. -
Sheet1
https://www2.mrc-lmb.cam.ac.uk/groups/JYL/WWWrobots/LMB%20plates/Addit04b_AL-SEL-DP_Formatted.xlsx24 Nov 2020: C12E9. APO129? 0.05. 583. E. 11. 59. Polyoxyethylene(10)dodecyl ether. 20. C12E10. ... AP1210? 0.1. 627. E. 12. 60. Polyoxyethylene(8)tridecyl ether. 20. C13E8. APO138? -
31/10/2018 Carter lab TIRF microscopy Single Molecule Assay 5x ...
https://www2.mrc-lmb.cam.ac.uk/groups/cartera/download/resources/protocols/2020_Carter_TIRF_protocol.docx28 Jan 2020: 15 mg Glucose Oxidase 2mg Catalase (both in -20) into 250 μL DLB. ... Don’t pipette up and down. Could use a cut 20 μL tip for this. -
Carter lab insect cell ZZ-tag prep (2020) Buffers Wash ...
https://www2.mrc-lmb.cam.ac.uk/groups/cartera/download/resources/protocols/2020_Carter_Sf9_dynein_prep.docx24 Jan 2020: Gel-filtration on AKTAmicro. -Column is stored in water 0.05% NaN3. If stored in 20% ethanol, first wash into water before washing into buffer. ... Remove column and replace caps on either end. -Put inlet A1 into 20% ethanol, pumpwash. -
16001658465475 1..36
https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/Collier_2020_elife-59560-v1.pdf15 Sep 2020: reagent bis-maleimidoethane (BMOE). Around 20% of cohesin complexes can be crosslinked simul-. ... teins were denatured by adding SDS to a final concentration of 1% and heating at 70˚C for 20 min.
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