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Activation of GCN2 by the ribosomal P-stalk
https://www2.mrc-lmb.cam.ac.uk/groups/hegde/download/PNAS_2019.pdf7 Mar 2019: Upon deletion of the CTD (construct C), GCN2’sbasal activity increased 20-fold (Fig. ... 0.001 0.01 0.1 1 10 1000. 10. 20. 30. 40. Time (min). % -
The structure of bactofilin filaments reveals their mode of membrane…
https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/Deng2019Natmicro.pdf26 Aug 2019: 80. 85. 90. 95. 5. 15. 5. 20. 15 80 85 90 95. ... in a 45 Ti rotor (Beckman) for 30 min at 4 C, followed by another centrifugation at 20,000 r.p.m. -
The ER membrane protein complex promotes biogenesis of sterol-related …
https://www2.mrc-lmb.cam.ac.uk/groups/hegde/download/JCS_2019.pdf18 Jan 2019: A) WT and ΔEMC6 (Δ6) cells were exposed to Chol:MBCD (25, 37.5 and50 µM, 20 h) and visualised by staining with Crystal Violet. ... or without 10 µM avasimibe (AVA, 20 h)and visualised by staining with Crystal Violet. -
Articleshttps://doi.org/10.1038/s41594-019-0196-z 1MRC Laboratory of…
https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/Burmann%202019%20NSMB.pdf4 Mar 2019: 20 nm. 20 nm. 20 nm. 20 nm. 20 nm. 20 nm 20 nm 20 nm. ... 20 nm 20 nm 20 nm20 nm. 20 nm 20 nm 20 nm. -
Biallelic Variants in ASNA1, Encoding a Cytosolic Targeting Factor of …
https://www2.mrc-lmb.cam.ac.uk/groups/hegde/download/CircGen_2019.pdf1 Dec 2019: Both missense variants were predicted to be del-eterious (CADD >20 and M-CAP >0.025). ... buffer supplemented with 20 mM imidazole, and eluted with lysis buffer supplemented with 250 mM. -
TTC5 mediates autoregulation of tubulin via mRNA degradation
https://www2.mrc-lmb.cam.ac.uk/groups/hegde/download/Science_2019.pdf19 Nov 2019: Methods Mol. Biol. 619, 339–363 (2010). doi:10.1007/978-1-60327-412-8_20 Medline. 36. Q. Feng, S. ... Shao, In vitro reconstitution of translational arrest pathways. Methods 137, 20–36 (2018). -
15591370691369 1..30
https://www2.mrc-lmb.cam.ac.uk/groups/hegde/download/elife_2019.pdf29 May 2019: body binding is 4.4% (i.e., 20-fold less than our fluorescence standard). ... 20. 40. 60. 80. 0. Nb uptake by GFP-PrP. (Tg x 2h). -
Sub ForwardPrimer() ' ' ForwardPrimer Macro ' '…
https://www2.mrc-lmb.cam.ac.uk/groups/cartera/download/resources/computer/word_macros/UsefulMacros.docx25 Jan 2019: Sub ForwardPrimer(). '. ' ForwardPrimer Macro. '. '. Selection.MoveRight Unit:=wdCharacter, Count:=20, Extend:=wdExtend. ... Selection.Font.Underline = wdUnderlineSingle. Selection.MoveLeft Unit:=wdCharacter, Count:=1. End Sub. Sub ReversePrimer(). '. ' -
Recognition and Degradation of Mislocalized Proteins in Health and…
https://www2.mrc-lmb.cam.ac.uk/groups/hegde/download/CSHL_Persp_2019.pdf8 Mar 2019: Recognition and Degradation of MislocalizedProteins in Health and Disease. Ramanujan S. Hegde and Eszter Zavodszky. Medical Research Council Laboratory of Molecular Biology, Cambridge CB2 0QH, United Kingdom. Correspondence: -
Cryo‐EM structure of the MinCD copolymeric filament from Pseudomonas…
https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/Szewczak-Harris_et_al-2019-FEBS_Letters.pdf22 Jul 2019: and therefore its dissociation from the membrane. [19,20]. Released MinD will then undergo exchange of. ... spatially regulate cytokinesis. Cell 146, 396–407.20 Vecchiarelli AG, Li M, Mizuuchi M, Hwang LC,.
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