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28 Nov 2008: After a 20,000g centrifugation the supernatant was analysed by SDS-PAGE and immunoblotting. ... The lysate was centrifuged at 20,000g for 20 minutes at 4C and the protein concentration in the supernatant was measured using the BCA Protein Assay Kit

30 May 2008: clustering in three distinct domains: a) nine functional PT sequences appear in the amino terminal 20% of apoB, b) four more PT sequences occur just before the end of apoB48, and ... Conversely, two large regions, apoB- 20-44 and apoB-47-67, each

22 Jul 2008: 1 C , underlined). This tolerability in specifi c sequence was strikingly illustrated by the observation that up to one-fi fth of all random 20-residue sequences can serve as ... Submitted: 28 April 2008 Accepted: 20 June 2008. References Besemer , J. ,

18 Sep 2008: the Ifn-PrP mice at15%–20% (if all of the Ifn-PrP was being de-graded by this pathway). ... the range of 10%–20% of endogenous PrP levels. These results indicate that Ifn-PrP mice mimic the situation ex-.

22 May 2008: PtdIns(4,5)P2 is rapidlyhydrolyzed upon stimulated secretion, result-ing in reduced adhesion, and this is alsocoupled with a reduction in the force re-quired for tether formation (20). ... An increased surfacetension reduces endocytosis (20).

4 Mar 2008: enterocolitica FtsQ, in 20 mM Tris,1 mM EDTA, 1 mM NaAzide, 50 mM NaCl, pH 7.0 (TEN50)for S. ... aureus FtsQ and in 20 mM Tris, 1 mM EDTA, 1 mMNa-azide, pH 7.5 (TEN7.5) for C.

16 May 2008: From extensive research over the past 20 years, the following consensus on SNAre function has emerged.

16 Jul 2008: For EndoH digestion, samples were cooled and diluted with G5buffer (New England Biolabs) to a final concentration of 0.1% SDS, 20 mMTris, and 50 mM citrate, pH 5.5, before