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Transferrin-Uptake Assay
https://www2.mrc-lmb.cam.ac.uk/groups/hmm/techniqs/Transferrin%20Uptake%20Assay.html21 Feb 2008: serum free medium to cells and incubate at 37 ¡C for the desired time (15-20 minutes for steady state). ... For transferrin alone, use 4¡C. Important: Fix cells with 3.2 % paraformaldehyde pre-equilibrated in serum-free medium at the required -
Dlp Purification:
https://www2.mrc-lmb.cam.ac.uk/groups/hmm/techniqs/Eva%20Methods.htm6 Feb 2008: 1: 5 000. poly. Endophilin1. Ra 37. poly. Eps15. Santa Cruz (C-20). ... Five to 20 cells were co-plated with K. aeorogenes on SM agar plates. -
Sup Fig 1
https://www2.mrc-lmb.cam.ac.uk/groups/hmm/publica/YopJSup.pdf11 Nov 2008: phospho IκB. IκB. YopJ-wt. TNF-α (min) 0 5 10 15 20 0 5 10 15 20. ... YopJ-C172A YopJ-wt. 0 5 10 15 20 0 5 10 15 20 TNF-α (min). -
HARVEY T McMAHON CV
https://www2.mrc-lmb.cam.ac.uk/groups/hmm/people/CV2008c.htm28 Jul 2008: EMBO J. 13, 5051-5061 (1994). 20. McMahon, H.T. and SŸdhof, T.C. ... Biochemical Journal 414, 189-194. Highlights of 20 papers above marked with an asterix. -
www.sciencemag.org/cgi/content/full/1164346/DC1 Supporting Online…
https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/Salje%20et%20al%20Science%202009%20Suppl.pdf15 Dec 2008: nitrogen cooling, FEI company, Eindhoven, NL) equipped with a Gatan energy filter (filter bandpass 20 eV). ... 18. Figure S4 APlasmid-free control. 19. Figure S4 BPlasmid-free control. 20. -
PII: 0304-3991(84)90197-9
https://www2.mrc-lmb.cam.ac.uk/groups/nu/pdf/ultram84.pdf26 Oct 2008: Prior to transfer the bag is sealed and dry nitrogen gas is flushed through it for about 20 s. -
molcel2806mmc1
https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/FtsK%20MolCell%202008%20Lowe%20Supp%20data.pdf16 Jul 2008: ASCII85EncodePages false /AllowTransparency false /AutoPositionEPSFiles true /AutoRotatePages /All /Binding /Left /CalGrayProfile (Dot Gain 20%) /CalRGBProfile (sRGB IEC61966-2.1) /CalCMYKProfile (U.S. -
Bacterial actin: architecture of the ParMRC plasmidDNA partitioning…
https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/ParMRC%20Salje%202008%20EMBO.pdf4 Aug 2008: least 20 ParR C-terminal tails present, producing a very high. local concentration. ... Under these condi-. tions, rings of roughly 20 nm diameter could be observed. -
No Job Name
https://www2.mrc-lmb.cam.ac.uk/groups/hegde/download/49_MacKinnon_AL_JACS_2007.pdf30 May 2008: described)1 were incubated with or without 2 (500 nM) and 3 (0–20 µM, final. ... 20 mL) and the combined organic fractions were washed with saturated NaCl solution. -
Membrane Localization is Critical for Activation of the PICK1 BAR…
https://www2.mrc-lmb.cam.ac.uk/groups/hmm/publica/MadsenGetherTraffic2008.pdf18 Oct 2008: modules (19,20). The interaction with lipids has been. suggested to be mediated by electrostatic attraction. ... position (20). We note the possibility that while the. situation in PICK1 is somewhat different, the mechanism.
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