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24 Sep 2007: º) 90, 106.5, 90. Wavelength 0.97962. Resolution (Å) 20 – 3.1 (3.2 – 3.1). ... www.nature.com/nature 20. Supplementary notes / Supplementary References. 23. Lenzen, C., Cool, R.

14 Jun 2007: Recruitment of ESCRT-0 to the endosomal membrane. The N-terminal FYVE domain of HRS mediates recog-nition of PtdIns3P, a highly abundant phosphoinositide in endosomal membranes18–20. ... Clathrin acts as an organizer of the ESCRT pathway. HRS is highly

15 Aug 2007: For fractionation, cytosolic proteins were selectively extracted on icefor 5 min with 1 ml/35-mm dish of 100 g/ml digitonin in KHM buffer (110 mMKAc, 20 mM HEPES, pH ... Cross-linker was quenched with 20 mM Tris-Cl, pH 7.5, the cells were lysed in1% SDS

11 May 2007: If using antibodies to label the protein(s) ofinterest, either monoclonal or mono-specificpolyclonal antibodies (raised against shortpeptide sequences of 8 to 20 amino acids)are preferable to broad specificity ... While the overall trends and

15 May 2007: 14,19,20). The IVF motif also contributes to the intra-. molecular interaction but with significant difference among.