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LETTERS A bacterial dynamin-like proteinHarry H. Low1 & Jan ...
https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/nature%20BDLP%202006.pdf28 Nov 2006: 100 120. kDa 220. Nosto. c p. ly. sate. M. 0 0 0.2 0.4 0.6 0.8 1.0 1.2 40 80 60 20. ... 60. 40. 0. 20. 80. WT K82A mutant. 0.2 0.4 0.6 0.8 1.0 1.2. -
doi:10.1016/j.jbbm.2005.12.008
https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/rfcloning2006%20published.pdf19 Apr 2006: 2.2. Linear amplification reaction. 2–20 nM of PCR product was used in a linear amplification reaction with 6–400 pM ofpHis17 (B. ... The protein was purified over a Ni-NTA spin column under native conditions (lanes 3 and 5) and separated on a 10–20 -
� Supplementary Figure 1 Bacterial dynamin-like protein (BDLP) from…
https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/nature%20BDLP%202006%20sup.pdf28 Sep 2006: The lysate buffer consisted of 50 mM Tris-Cl, 1.25 M sodium chloride, final pH 8.0, the wash buffer of 50 mM Tris-Cl, 500 mM sodium chloride, 20% ... For the phylogenetic tree, 10-20 sequences related to the different classes of dynamin-related proteins -
NPGRJ_NSMB_1158 1..8
https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/nsmbFtsK2006.pdf12 Oct 2006: Radiolabeled 20-bp fragmentsthat lacked KOPS (nKOPS) or that contained a single KOPS (sKOPS). ... 50 l culture. NMR buffer was 20 mM potassium phosphate and 150 mM. -
Supplementary Figure 1 EPR spectra of R1-labeled endophilin A1 ...
https://www2.mrc-lmb.cam.ac.uk/groups/hmm/publica/GallopEndophilinEMBO/EndoSupFigures.pdf8 Jun 2006: Readings were performed at 20 C using an average of 5 scans with buffer subtracted. ... uals. 6.00 10 20Concentration (µM). 0. 20. 40. 60. 80 (k. -
© 2005 Nature Publishing Group Endophilin and CtBP/BARS are ...
https://www2.mrc-lmb.cam.ac.uk/groups/hmm/publica/GallopEndopohilinNature2005.pdf4 Jan 2006: fatty acid-free BSA and 0.01% Triton X-100 with substrate concentrations (20–200 mM LPA) at 25 8C and 37 8C with 14C-labelled oleoyl-CoA (Amersham) at aspecific ... Liposomes made from Folch brain lipid extract at0.2 mg ml21 in 20 mM HEPES pH 7.4, 150 -
Acetylation of MEK2 and I{kappa}B kinase (IKK) activation loop…
https://www2.mrc-lmb.cam.ac.uk/groups/hmm/publica/YopJ.pdf28 Nov 2006: 2000) J Biol Chem 275:36062–36066.20. Jeon KI, Byun MS, Jue DM (2003) Exp Mol Med 35:61– 66.21. ... Twenty-four hours after transfection, cells were serum-starved for 16 h and then stimulated with 20 ng/ml TNF-α. -
1202.tif
https://www2.mrc-lmb.cam.ac.uk/groups/nu/pdf/jcb84.pdf16 Jun 2006: The amino acid sequences deduced from recent cDNA sequences (7, 10, 19, 20) indicate molecular weights of 50,000 (a), 54,000 (/), 56,000 (30, and 58,000 (6), and show ... Nature (Lond.). 299:793-797. 20. Noda, M., H. Takahashi, T. Tanabe, M. -
1 Supplementary data to accompany paper: Mechanism of endophilin ...
https://www2.mrc-lmb.cam.ac.uk/groups/hmm/publica/GallopEndophilinEMBO/EndoSupMethods.pdf8 Jun 2006: analytical ultracentrifuge with an An60-Ti rotor, with 39, 78 and 156 µM in 150 mM NaCl, 20. ... at 40 and 150 µM protein in 150 mM NaCl, 20 mM HEPES pH 7.4, 2 mM DTT unless. -
doi:10.1016/j.molcel.2006.06.019
https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/Ftsk%20mc%202006.pdf12 Aug 2006: 50 mM bp), and PaFtsK (20 mM monomer) were mixed in 20 mM. ... ine, and 8% (w/v) PEG 2000. Cryoprotectant: 20% PEG 550 MME in.
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