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26 Jan 2006: Dialyze into 150 mM NaCl, 20 mMHEPES pH 7.4, 0.4 mM DTT for use. ... 1998), dialyzed into HKMbuffer, and centrifuged for 20 min at 100,000g immediately prior to useto remove aggregates.

7 Dec 2006: to polyvinylidene difluoride membrane for3.5 h at 20 mAmps using a wet transfer apparatus (Trans-BlotTMCell, Bio-Rad). ... Genes Dev 20: 113–124. Kunst, F., and Rapoport, G. (1995) Salt stress is an environ-mental signal affecting degradative enzyme

8 Sep 2006: of the motor domain is rotated by 20 betweenthe ADP and AMPPNP states. ... 1 mM DTT, 0.5 mM GTP, and 10% DMSO), stabilized with 20 mM.

17 Jan 2006: Here, full-length recombinant SNX2 wasincubated with liposomes comprising phosphatidylcholine(PtdCho), phosphatidylserine (PtdSer) andphosphatidylethanolamine (PtdEth) (each at 26.6% (w/w)) towhich 20% (w/w) of the relevant phosphoinositide was added

4 Jan 2006: Membrane curvatureresponse. d-Amph 3-20. Amphipathic helices are stretches of -helix, one side of which is polar(charged) and the other hydrophobic. ... Proc. Natl Acad. Sci.USA 101, 8262–8269 (2004). 20. Honing, S. et al.

12 Nov 2006: 66). We then measured the binding of PI3K-C2α PX mutants to POPC/POPE/PtdIns(4,5)P2 (78:20:2) vesicles. ... 1996) J Biol Chem 271, 13892-13899. 20. Virbasius, J. V., Guilherme, A., and Czech, M.

19 Jun 2006: Annu Rev Cell Dev Biol 20:395–425. Seeger H, Heikenwalder M, Zeller N, Kranich J, Schwarz P, Gaspert A, Seifert B, Miele G, Aguzzi A (2005) Coincident scrapie infection andnephritis

12 Nov 2006: 1 B). All the complete sequences analyzed in Fig. 1 have between 20 and 24 continuous repeats. ... 1 B). Other repeat lengths spread the corresponding amino acids at 20 intervals through the circum-ference of the helix.

12 Nov 2006: Significantly, four different tumoursuppressor genes act to suppress mTor activity (TSC1,TSC2 [19], LKB-1 [20] and VHL [21], see Figure 2). ... Trends Biochem Sci 2003, 28:573-576. 20. Shaw RJ, Bardeesy N, Manning BD, Lopez L, Kosmatka M,DePinho RA,

21 Sep 2006: 4†. (F) Cst 3 Puro 10 0.7 0.2ER-GFP (C) Steady state 20 8.7 2.5. ... H) Cst 20 5.6 1.7(E) Puro 3 Cst 8 8.5 2.3†.