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Organization of spines on the dendritesof Purkinje cellsJohn O’Brien…
https://www2.mrc-lmb.cam.ac.uk/groups/nu/pdf/pnas06.pdf25 Jul 2006: The fish dendrites are straighter and less branched than theothers, but some weaver dendrites are also straight over lengths of 20 –30! ... mM NaCl"10 mM KCl"2 mM CaCl2"1 mMMgCl2"2 mM NaHCO3"4 mM Hepes, pH 7.4, supplementedwith Medium 199 (Sigma) in a -
MD1-91, MD1-92 and MD1-92-FX Morpheus II 10 mL, HT-96 and FX-96…
https://www2.mrc-lmb.cam.ac.uk/screens2/pdf/LMB20.pdf9 Jul 2018: MD2-100-240. MD2-250-240. 60%Precipitant Mix 7 20% w/v PEG 8000, 40% v/v 1,5-Pentanediol. ... B7 2 mM Divalents II none 30 % v/v Precipitant Mix 71-20 B8 2 mM Divalents II none 31 % v/v Precipitant Mix 81-21 B9 2 mM Divalents II -
Structural and Membrane Binding Analysis of the PhoxHomology Domain…
https://www2.mrc-lmb.cam.ac.uk/groups/rlw/download/publications/17581820.pdf8 Dec 2007: However, K338Aand R349A showed 3- and 4-fold reduced affinity, respectively,for more anionic POPC/POPE/POPS/PtdIns(4)P (57:20:20:3)vesicles. ... 3)P (77:20:3) (f), POPC/POPE/PtdIns(5)P (77:20:3) (Œ),and POPC/POPE/POPS (60:20:20) (") monolayers. -
Addit04b_AL-SEL-DP Formated
https://www2.mrc-lmb.cam.ac.uk/screens2/pdf/Addit-04b.pdf24 Nov 2020: Z3-12 AZ312 2.8 335.5A 5 5 ANZERGENT 3-14 20 Z3-14 AZ314 0.2 363.6A 6 6 n-Decyl-N,N-dimethylglycine 76 DMG D352 19 ... 0.05 583E 11 59 Polyoxyethylene(10)dodecyl ether 20 C12E10 AP1210? 0.1 627E 12 60 Polyoxyethylene(8)tridecyl ether 20 C13E8 APO138? -
Dynamin architecture—from monomer to polymer
https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/dynaminreviewCOSBILow2010.pdf21 Oct 2010: Current Opinion in Structural Biology 2010, 20:1–8. 2 Proteins. COSTBI-829; NO. ... DynaminQ1 architecture — from monome. Current Opinion in Structural Biology 2010, 20:1–8. -
Addit05_NeXtal pH gradient
https://www2.mrc-lmb.cam.ac.uk/screens2/pdf/Addit-05.pdf20 Nov 2020: Sodium Citrate 1 MC6 18 5.6 tri-Sodium Citrate 1 MD1 19 5.8 tri-Sodium Citrate 1 MD2 20 6 Sodium Cacodylate 1 MD3 21 6.2 Sodium Cacodylate -
Structural Mapping of the Catalytic Mechanism for a…
https://www2.mrc-lmb.cam.ac.uk/groups/rlw/download/publications/9048554.pdf20 Apr 2002: 4.6)〈I/σ(I)〉b 24.1(5.1) 15.7(4.3) 20.6(4.4) 17.2(4.9). ... 2.95Rfactor(%)a 21.7 20.0 21.5 21.2Rfree(%)b 27.2 25.7 27.0 27.4no. -
Pi-PEG Screen 1.5 disclaimer
https://www2.mrc-lmb.cam.ac.uk/screens2/pdf/LMB19.pdf28 Sep 2022: MME 8.55 % 1-19 PEG 300 25.65 % HEPES 75 mM 7.1 PEG 600 8.55 % 1-20 PEG 350 MME 25.65 % HEPES 75 mM 7.3 PEG ... 15 % 2-19 PEG 600 8.55 % HEPES 75 mM 7.1 PEG 300 42.9 % 2-20 PEG 1000 7.5 % HEPES 75 mM 7.3 PEG 350 MME -
cde235 2454..2461
https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/MinC%20EMBO%202001.pdf4 May 2001: Peak fractionswere pooled and concentrated before loading onto a Sephacryl S200column (Amersham Pharmacia) equilibrated in 20 mM Tris, 1 mM EDTAand 1 mM NaN3 pH 7.0. ... Proc. NatlAcad. Sci. USA, 92, 43134317. Received February 19, 2001; revised March 20, -
JCSG+ Screen
https://www2.mrc-lmb.cam.ac.uk/screens2/pdf/LMB12.pdf2 Oct 2014: 20 0.2 M Li2SO4 8% PEG 20,000 8% PEG 500 MME. ... 21 0.2 M MgCl2 8% PEG 20,000 8% PEG 500 MME. -
Collaborative protein filaments
https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/ghosal_embo_2015.pdf4 Jan 2016: Curr Opin Struct Biol 20: 791 – 798. Low HH, Sachse C, Amos LA, Löwe J (2009) Structure of a bacterial dynamin-. -
wiz12b_rigaku_20131116
https://www2.mrc-lmb.cam.ac.uk/screens2/pdf/LMB02.pdf2 Oct 2014: plate). EB‐W12‐BWell Precipitation Reagent Buffer Salt. A1 20% (w/v) PEG 8000 100 mM CHES/ Sodium hydroxide pH 9.5. ... C11 20% (v/v) 1,4‐butanediol 100 mM Sodium acetate/ Acetic acid pH 4.5. -
©20 10 N atur e A mer ica, Inc. ...
https://www2.mrc-lmb.cam.ac.uk/groups/rlw/download/publications/20081827.pdf2 Aug 2010: p110 )19, rheumatoid arthritis (p110 and p110 )20 and asthma (p110 and p110 )21,22. ... Nat. Med. 11, 507–514 (2005). 20. Rommel, C., Camps, M. & Ji, H. -
2-126 Formulations
https://www2.mrc-lmb.cam.ac.uk/screens2/pdf/LMB05.pdf2 Oct 2014: 1. 20% w/v Polyethylene Glycol 33502. 20% w/v Polyethylene Glycol 33503. ... 20% w/v Polyethylene Glycol 335016. 20% w/v Polyethylene Glycol 3350 17. -
J. Mol. Biol. (1974) 87, 641-656 Electron Microscopy of ...
https://www2.mrc-lmb.cam.ac.uk/groups/nu/pdf/jmb74.pdf26 Jun 2024: Its existence was first inferred nearly 20 years ago (Franklin & Commoner, 1965) from X-ray diffraction patterns of the polymerized protein isolated from infected plants, and it is now known (Finch & ... Typically, in this way, we were able to make -
Manual_CO-311 JBScreen Solubility HTS
https://www2.mrc-lmb.cam.ac.uk/screens2/pdf/SOLUB.pdf19 Mar 2018: In addition, a positive control for precipitation (20% acetonitrile, well# A1) is included in the screen. -
JCSG+ Screen
https://www2.mrc-lmb.cam.ac.uk/screens2/pdf/LMB11.pdf2 Oct 2014: 20 0.2 M Li2SO4 8% PEG 20,000 8% PEG 500 MME. ... 21 0.2 M MgCl2 8% PEG 20,000 8% PEG 500 MME. -
Angstrom Additive Screen Scoring Sheet MD1-100.xlsx Angstrom Additive …
https://www2.mrc-lmb.cam.ac.uk/groups/JYL/WWWrobots/LMB%20plates/Addit_02.pdf26 Nov 2015: F6 Pentaethylene glycol, 80% v/v. F7 PEE (3/4 EO/OH), 20% w/v. ... Well # Tube Contents. G1 PEG 300, 20% v/v. G2 PEG 300, 40% v/v. -
Impress
https://www2.mrc-lmb.cam.ac.uk/coot/ephemera/beyond-coloured-lines.pdf28 Sep 2020: detailed, interactive representation of “high energy”. 20 / 50. Colour-blindness Considerations. ... Slide 18. Slide 19. Slide 20. Slide 21. Slide 22. Slide 23. -
The Journal of Cell Biology © The Rockefeller University ...
https://www2.mrc-lmb.cam.ac.uk/groups/hmm/epsinR/sedequil.pdf16 Jan 2003: The Journal of Cell Biology The Rockefeller University Press • 0021-9525Volume 160 January 20, 2003http://www.jcb.org/cgi/doi/10.1083/jcb.200208023. -
MD1-118 Hippocrates additive screen
https://www2.mrc-lmb.cam.ac.uk/screens2/pdf/Addit-03.pdf9 Jul 2018: C1 13 9 % Pyridoxine hydrochloride C2 14 9 % Thiamine hydrochloride C3 15 9 % CytidineC4 16 6 % Inosine†C5 17 6 % RibavirinC6 18 6 % ThymidineD1 19 6 % UridineD2 20 1.5 % -
MD1-46 Morpheus
https://www2.mrc-lmb.cam.ac.uk/screens2/pdf/LMB23.pdf4 Aug 2021: Catalogue Number (250 mL). 60% Precipitant Mix 1 P500MME_P20K 40% v/v PEG 500 MME; 20 % w/v PEG 20000. ... MD2-100-82 MD2-250-82. 60% Precipitant Mix 3 GOL_P4K 40% v/v Glycerol; 20% w/v PEG 4000. -
MD1-116-117 Morpheus III-Morpheus III HT-96
https://www2.mrc-lmb.cam.ac.uk/screens2/pdf/LMB22.pdf9 Jul 2018: Catalogue Number (250 mL). 60% Precipitant Mix 1 40% v/v PEG 500 MME; 20 % w/v PEG 20000. ... MD2-100-82 MD2-250-82. 60% Precipitant Mix 3 40% v/v Glycerol; 20% w/v PEG 4000. -
The Journal of Cell Biology © The Rockefeller University ...
https://www2.mrc-lmb.cam.ac.uk/groups/hmm/publica/sedequil.pdf16 Jan 2003: The Journal of Cell Biology The Rockefeller University Press • 0021-9525Volume 160 January 20, 2003http://www.jcb.org/cgi/doi/10.1083/jcb.200208023. -
MD1-100 The Angstrom Additive Screen
https://www2.mrc-lmb.cam.ac.uk/screens2/pdf/Addit-02.pdf23 Sep 2016: propane. In addition to being well-suited as crystallization reagents, these five polyols are cryo-protectants when used at concentrations as low as 20–25% (w/v). ... Xylitol D10 20 % w/v D-Fructose -D11 40 % w/v D-Fructose D12 80 % w/v D-Fructose. -
MD1-46, MD1-47 and MD1-47-FX Morpheus 10 mL, HT-96 and FX-96…
https://www2.mrc-lmb.cam.ac.uk/screens2/pdf/LMB17.pdf9 Jul 2018: 60% Precipitant Mix 1 P500MME_P20K 40% v/v PEG 500 MME; 20 % w/v PEG 20000. ... MD2-100-82 MD2-250-82. 60% Precipitant Mix 3 GOL_P4K 40% v/v Glycerol; 20% w/v PEG 4000. -
Chapter 20 In Vitro Dissection of Protein Translocationinto the ...
https://www2.mrc-lmb.cam.ac.uk/groups/hegde/download/62_Sharma_AS_MMB_2010.pdf30 Apr 2010: 20.3). Removal of thedetergent can be accomplished in several ways including sim-. ... Repeat this extensively (20 or moretimes) until all traces of MeOH are removed. -
LMB Screen Scoring Sheet
https://www2.mrc-lmb.cam.ac.uk/groups/JYL/WWWrobots/LMB%20plates/LMB_21.pdf23 Sep 2016: G9 20% w/v PEG 8000, 0.2M magnesium acetate, 0.1M MES pH 6.5. ... G11 20% w/v PEG 8000, 0.2M magnesium chloride, 0.1M CAPS pH 9.0. -
Morpheus Additive Screen Scoring Sheet MD1-93.xlsx Morpheus Additive…
https://www2.mrc-lmb.cam.ac.uk/groups/JYL/WWWrobots/LMB%20plates/Addit_01.pdf26 Nov 2015: A3 PEG 500 MME precipitant, 40% w/v. A4 PEG 8000 precipitant, 20% w/v. ... A5 Ethylene glycol cryoprotectant, 40% v/v. A6 PEG 4000 precipitant, 20% w/v. -
Expression of selenomethionine substituted proteins innon-methionine…
https://www2.mrc-lmb.cam.ac.uk/groups/JYL/methods/SeMet%20recipe.doc.pdf2 Feb 2004: Add reducing agents immediately before use. Minimal medium (per litre):1l M9 medium2ml 1M MgSO4 (2mM)20ml 20% glucose (0.4%)antibiotic(s)1ml vitamins 1000x (see below)10ml trace elements -
Influence of lipid bilayer on the structure of the muscle-type…
https://www2.mrc-lmb.cam.ac.uk/groups/nu/pdf/pnasS24.pdf30 Apr 2024: Nat. Commun. 14, 3169 (2023). 20. P. Kumar et al., Cryo- EM structures of a lipid- sensitive pentameric ligand- gated ion channel embedded in a phosphatidylcholine- only bilayer. ... Electron Microscopy Data Bank https://www.ebi.ac.uk/emdb/EMD-18840. -
crib-sheet.dvi
https://www2.mrc-lmb.cam.ac.uk/personal/pemsley/coot/files/crib-sheet.pdf14 Oct 2016: i: Toggle continuous spin. 1.20 Baton Mode. b: toggle into baton rotate mode4. -
Alonsoetal Supplementary Material 030107
https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/Alonso-suppmat.pdf2 Apr 2007: Purified tubulins were desalted into PEM containing 20 μM GDP before storage in liquid nitrogen. ... Enzyme reactions were done in 20 mM PIPES pH 6.9, 2 mM MgCl2, using quartz fluorimeter cuvettes. -
HiroseSuppl
https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/supp_MC2006.pdf25 Aug 2006: The thickness of the. sections is 15 Å in (A-F), and 20 Å in (G-I). ... was reported to rotate clockwise by 20 in the ADP state compared with the AMPPNP state. -
2-213 Formulations
https://www2.mrc-lmb.cam.ac.uk/screens2/pdf/LMB04.pdf2 Oct 2014: pH. 4 5 6 7 8 9. 5. 10. 20. 30. ... pH. 4 5 6 7 8 9. 10. 20. 40. 65. -
Endocytosis: Curvature proteins direct traffic
https://www2.mrc-lmb.cam.ac.uk/groups/hmm/publica/Papers/NRMCB_Fcho.pdf18 Jun 2010: 20 Macmillan Publishers Limited. All rights reserved10. -
DOI: 10.1126/science.1136985 , 120 (2007); 316Science et al.Maria C.…
https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/Alonso_Science.pdf6 Apr 2007: 0 5 10 15 20. 0 5 10 15 20. 0 5 10 15 20-10. ... Themicrotubule plus end is toward the top of thepage. (Left) Cryo-EM map (20). -
�������� ��� � ���� �� �� ���� ��� ������ ...
https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/FtsA%20EMBO%20.pdf4 Oct 2000: H8 B 20 # 4992# -. 0 B A , ' ( 0+ 3 $ I(E (2 Q 3(< , % 0 B 4412S# , / 3BB+ 0 B 4912# - , ' # ( % # % $ # - / , (B<- 0 4412#. " "! ' ( < -
KWRM_A_992668_O
https://www2.mrc-lmb.cam.ac.uk/groups/wschafer/2015-3.pdf20 Oct 2015: 20. Oct. ober. 201. 5. neurotransmitter release or receptionmechanisms is the most traditional andwidely used. ... 1:07. 20. Oct. ober. 201. 5. circuits, reprogram their function andchange the way they control behavior. -
Molecular basis for membraneremodelling and organization Deadline for …
https://www2.mrc-lmb.cam.ac.uk/groups/hmm/F-BAR_proteins/Le%20Poster_DEUXNew.pdf29 Sep 2011: Molecular basis for membraneremodelling and organization. Deadline for application is 20 May 2011. -
Protocol for Hamilton LABSTAR
https://www2.mrc-lmb.cam.ac.uk/groups/JYL/WWWrobots/PDF/Manuals/Protocol_for_LabStar.pdf11 Dec 2017: PROTOCOL System 2: setting up crystallization droplets (100 nL protein 100 nL condition) from a single sample in 20 LMB plates 1. ... 15. Clean the SBS lids with a 20% ethanol solution before stacking them on the left-hand side of the liquid handler for -
molcel3956mmc1
https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/Salje2011Supp.pdf20 Jul 2011: Cells were lysed in buffer A (20 mM Tris pH 8.5, 500 mM NaCl, 0.1 mM EDTA), supplemented with DNase (Sigma) and protease inhibitor tablets (Roche) by passing it ... J Biochem Biophys Methods 67, 67-74. << /ASCII85EncodePages false /AllowTransparency -
FigS1
https://www2.mrc-lmb.cam.ac.uk/groups/hmm/publica/SchmidBetaPaper/BetaHubSup.pdf7 Sep 2006: 15%out of 132 residues. 22%out of 114 residues. 20%out of 237. ... residues. 20%out of 128 residues. 128 residues in total. hβ4-appendage. 237 residues in total. -
crib-sheet.dvi
https://www2.mrc-lmb.cam.ac.uk/personal/pemsley/coot/web/docs/crib-sheet.pdf27 Aug 2015: i: Toggle continuous spin. 1.20 Baton Mode. b: toggle into baton rotate mode4. -
Nature © Macmillan Publishers Ltd 1998 8 30. Fan, ...
https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/FtsZ.Nature.pdf9 Jan 1998: Sequence comparisons between tubulins and FtsZ had alreadyrevealed limited homology of ,10–18% identity7,19,20. ... Proc. Natl Acad. Sci. USA 90, 1053–1057 (1993). 20. de Pereda, J. -
PII: 0304-3991(84)90197-9
https://www2.mrc-lmb.cam.ac.uk/groups/nu/pdf/ultram84.pdf26 Oct 2008: Prior to transfer the bag is sealed and dry nitrogen gas is flushed through it for about 20 s. -
Microscopy, 2021, 1–6doi:https://doi.org/10.1093/jmicro/dfab023…
https://www2.mrc-lmb.cam.ac.uk/groups/nu/pdf/mic21.pdf26 Jul 2021: A likely explanation, in line with other structuralevidence [13,20], is that bilayer thickness is modulated primarily bythe properties of the embedded protein, rather than by cholesterol,in regions where the ... Sci. Adv. 7: eabe6204. 20. Norimatsu Y, -
Date: 4-5th April 2011 Venue: School of Biosciences, University ...
https://www2.mrc-lmb.cam.ac.uk/groups/hmm/conferences/BirminghamProgram.pdf4 Mar 2011: Recommended Hotels: Menzies Strathallan 225 Hagley Road Birmingham, West Midlands B16 9RY 0121 455 9777 Price range: £50-60 per night Premier Inn Birmingham Broad St (Canal Side) 20 Bridge Street -
MD1-118 Hippocrates additive screen
https://www2.mrc-lmb.cam.ac.uk/groups/JYL/WWWrobots/LMB%20plates/Addit-03.pdf13 Oct 2020: C1 13 9 % Pyridoxine hydrochloride C2 14 9 % Thiamine hydrochloride C3 15 9 % CytidineC4 16 6 % Inosine†C5 17 6 % RibavirinC6 18 6 % ThymidineD1 19 6 % UridineD2 20 1.5 % -
Crystal Screen Lite™ is a complete reagent kit designed ...
https://www2.mrc-lmb.cam.ac.uk/screens2/pdf/LMB06b.pdf26 Jun 2015: 730 ml water 3. • 20 ml 2.0 M Calcium chloride dihydrate (CAS # 10035-04-8, Catalog # HR2-557). • ... 20. 0.2 M Ammonium sulfate, 0.1 M Sodium acetate trihydrate pH 4.6, 12.5% w/v Polyethylene glycol 4,000.
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