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11 Nov 2008: phospho IκB. IκB. YopJ-wt. TNF-α (min) 0 5 10 15 20 0 5 10 15 20. ... YopJ-C172A YopJ-wt. 0 5 10 15 20 0 5 10 15 20 TNF-α (min).

15 Dec 2008: nitrogen cooling, FEI company, Eindhoven, NL) equipped with a Gatan energy filter (filter bandpass 20 eV). ... 18. Figure S4 APlasmid-free control. 19. Figure S4 BPlasmid-free control. 20.

26 Oct 2008: Prior to transfer the bag is sealed and dry nitrogen gas is flushed through it for about 20 s.

16 Jul 2008: ASCII85EncodePages false /AllowTransparency false /AutoPositionEPSFiles true /AutoRotatePages /All /Binding /Left /CalGrayProfile (Dot Gain 20%) /CalRGBProfile (sRGB IEC61966-2.1) /CalCMYKProfile (U.S.

18 Dec 2008: F) The Saci1373MIM2 (red)/Saci1372 MIT(yellow) interaction isclosely relatedin structurewith the CHMP6 MIM2(green, extended)/VPS4AMIT (gray helices) inter-action (20). ... 19. M. D. Stuchell-Brereton et al., Nature 449, 740 (2007).20. C.

11 Aug 2008: Similar results wereobtained for Arf6, but Arf6 bound even more strongly to mem-branes in the absence of GTP, probably due to binding sites onthe protein core ([19,20] and results ... J. Biol. Chem. 280, 21661–21666. 20 Amor, J. C., Horton, J.

4 Aug 2008: least 20 ParR C-terminal tails present, producing a very high. local concentration. ... Under these condi-. tions, rings of roughly 20 nm diameter could be observed.

4 Mar 2008: enterocolitica FtsQ, in 20 mM Tris,1 mM EDTA, 1 mM NaAzide, 50 mM NaCl, pH 7.0 (TEN50)for S. ... aureus FtsQ and in 20 mM Tris, 1 mM EDTA, 1 mMNa-azide, pH 7.5 (TEN7.5) for C.

30 May 2008: described)1 were incubated with or without 2 (500 nM) and 3 (0–20 µM, final. ... 20 mL) and the combined organic fractions were washed with saturated NaCl solution.

16 Oct 2008: 0 25 50 75 100. Seg1. e. 0.02. 0.2. 2. 20. ... PI-103PP121DMSO PP1. pTyr. 20 5.01. 250. 310. 08. pAkt (308). pS6pAkt (473).

4 Aug 2008: different species, suggesting that each type may have specific. roles [11,12,14-20]. ... ted with outer dense fibers rather than with outer doublet. microtubules [11-20].

18 Oct 2008: modules (19,20). The interaction with lipids has been. suggested to be mediated by electrostatic attraction. ... position (20). We note the possibility that while the. situation in PICK1 is somewhat different, the mechanism.

3 Sep 2008: Healthcare). The resin was washed with buffer C (20 mM Tris [pH 7.5],. ... 100 mM NaCl, 2 mM b-mercaptoethanol) followed by buffer D (20 mM Tris.

16 Aug 2008: R factor, R freea 0.20 (0.27), 0.25 (0.36). B factorsb 40.3 Å2, 1.15 Å2. ... Cal (UK) at 10C. Protein and DNAs were in 20 mM Tris/HCl, 1 mM EDTA,.

17 Sep 2008: Microtubule assembly dynamics: newinsights at the nanoscale. Curr Opin Cell Biol 2008;20:64–70. ... ogliano J. The bacterial cytoskeleton. Curr Opin Cell Biol 2008;20:19–27.opp D, Narita A, Oda T, Fujisawa T, Matsuo H, Nitanai Y, et al.

10 Jun 2008: elegans genomic. sequence contains at least 20 genes (designated flp- genes, for FMRFamide-like peptide. ... 20. inactive phase. Genetic analysis indicated that goa-1 probably functions to regulate egg-.

13 Dec 2008: C2 domain-containing proteins, such as synaptotagmins (17),. and proteins of the reticulon and DP1/Yop1 families (20,21). ... flat membrane into tubules of ;20 nm diameter (11). SmallG-proteins Arf1 and Sar1 expose amphipathic a-helices uponexchange of

6 Mar 2008: rate of foraging [19, 20]. However, the precise nature of the foraging movements in wild-type and. ... 20. Some values are easily affected by noise or errors during image processing or capture.

28 Nov 2008: After a 20,000g centrifugation the supernatant was analysed by SDS-PAGE and immunoblotting. ... The lysate was centrifuged at 20,000g for 20 minutes at 4C and the protein concentration in the supernatant was measured using the BCA Protein Assay Kit

30 May 2008: clustering in three distinct domains: a) nine functional PT sequences appear in the amino terminal 20% of apoB, b) four more PT sequences occur just before the end of apoB48, and ... Conversely, two large regions, apoB- 20-44 and apoB-47-67, each

22 Jul 2008: 1 C , underlined). This tolerability in specifi c sequence was strikingly illustrated by the observation that up to one-fi fth of all random 20-residue sequences can serve as ... Submitted: 28 April 2008 Accepted: 20 June 2008. References Besemer , J. ,

18 Sep 2008: the Ifn-PrP mice at15%–20% (if all of the Ifn-PrP was being de-graded by this pathway). ... the range of 10%–20% of endogenous PrP levels. These results indicate that Ifn-PrP mice mimic the situation ex-.

22 May 2008: PtdIns(4,5)P2 is rapidlyhydrolyzed upon stimulated secretion, result-ing in reduced adhesion, and this is alsocoupled with a reduction in the force re-quired for tether formation (20). ... An increased surfacetension reduces endocytosis (20).

16 May 2008: From extensive research over the past 20 years, the following consensus on SNAre function has emerged.

16 Jul 2008: For EndoH digestion, samples were cooled and diluted with G5buffer (New England Biolabs) to a final concentration of 0.1% SDS, 20 mMTris, and 50 mM citrate, pH 5.5, before