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25 Jul 2006: The fish dendrites are straighter and less branched than theothers, but some weaver dendrites are also straight over lengths of 20 –30! ... mM NaCl"10 mM KCl"2 mM CaCl2"1 mMMgCl2"2 mM NaHCO3"4 mM Hepes, pH 7.4, supplementedwith Medium 199 (Sigma) in a

12 Jun 2006: but not for long.

14 Dec 2006: Christien explains. how it really works.

3 Aug 2006: Gamze getting the knack.

27 Feb 2006: Sterilise (heat B 98C, 20 min 121.5C). This is not OK for sugars. ... When up to speed (15K) start culture pump at speed '10' (may work at 20 as well), adjust so that eflux is clear (after debubbling).

29 Sep 2006: Binds accessory proteins via appendage domain. Cargo recognition. mu1 (A/B). Binds Yxx(bulky hydrophobic) cargo motifs [20]. ... J Biol Chem, 2000. 275(29): p. 21862-9. 20. Ohno, H., et al., Interaction of tyrosine-based sorting signals with

28 Mar 2006: Add to M9 prior to experiment (per litre):15NH4Cl 1g (can dissolve in 10 mL H2O and filter sterilise into media)20% glucose 20 mLvitamins 1 mLtrace elements 10 mLMgSO4 (1M) ... 10 mL of 20% arabinose for BAI cells). Purification:As normal, however

25 Aug 2006: The thickness of the. sections is 15 Å in (A-F), and 20 Å in (G-I). ... was reported to rotate clockwise by 20 in the ADP state compared with the AMPPNP state.

4 Jan 2006: ratio. n (D. 280). 0 10 20. 0.00. 0.05. 0.10. 0.15. ... n (D. 280). 0 5 10. 0.00. 0.10. 0.20. s (Svedbergs).

7 Sep 2006: 15%out of 132 residues. 22%out of 114 residues. 20%out of 237. ... residues. 20%out of 128 residues. 128 residues in total. hβ4-appendage. 237 residues in total.

28 Nov 2006: 100 120. kDa 220. Nosto. c p. ly. sate. M. 0 0 0.2 0.4 0.6 0.8 1.0 1.2 40 80 60 20. ... 60. 40. 0. 20. 80. WT K82A mutant. 0.2 0.4 0.6 0.8 1.0 1.2.

19 Apr 2006: 2.2. Linear amplification reaction. 2–20 nM of PCR product was used in a linear amplification reaction with 6–400 pM ofpHis17 (B. ... The protein was purified over a Ni-NTA spin column under native conditions (lanes 3 and 5) and separated on a 10–20

28 Sep 2006: The lysate buffer consisted of 50 mM Tris-Cl, 1.25 M sodium chloride, final pH 8.0, the wash buffer of 50 mM Tris-Cl, 500 mM sodium chloride, 20% ... For the phylogenetic tree, 10-20 sequences related to the different classes of dynamin-related proteins

12 Oct 2006: Radiolabeled 20-bp fragmentsthat lacked KOPS (nKOPS) or that contained a single KOPS (sKOPS). ... 50 l culture. NMR buffer was 20 mM potassium phosphate and 150 mM.

8 Jun 2006: Readings were performed at 20 C using an average of 5 scans with buffer subtracted. ... uals. 6.00 10 20Concentration (µM). 0. 20. 40. 60. 80 (k.

4 Jan 2006: fatty acid-free BSA and 0.01% Triton X-100 with substrate concentrations (20–200 mM LPA) at 25 8C and 37 8C with 14C-labelled oleoyl-CoA (Amersham) at aspecific ... Liposomes made from Folch brain lipid extract at0.2 mg ml21 in 20 mM HEPES pH 7.4, 150

16 Jun 2006: The amino acid sequences deduced from recent cDNA sequences (7, 10, 19, 20) indicate molecular weights of 50,000 (a), 54,000 (/), 56,000 (30, and 58,000 (6), and show ... Nature (Lond.). 299:793-797. 20. Noda, M., H. Takahashi, T. Tanabe, M.

28 Nov 2006: 2000) J Biol Chem 275:36062–36066.20. Jeon KI, Byun MS, Jue DM (2003) Exp Mol Med 35:61– 66.21. ... Twenty-four hours after transfection, cells were serum-starved for 16 h and then stimulated with 20 ng/ml TNF-α.

8 Jun 2006: analytical ultracentrifuge with an An60-Ti rotor, with 39, 78 and 156 µM in 150 mM NaCl, 20. ... at 40 and 150 µM protein in 150 mM NaCl, 20 mM HEPES pH 7.4, 2 mM DTT unless.

12 Aug 2006: 50 mM bp), and PaFtsK (20 mM monomer) were mixed in 20 mM. ... ine, and 8% (w/v) PEG 2000. Cryoprotectant: 20% PEG 550 MME in.

26 Jan 2006: Dialyze into 150 mM NaCl, 20 mMHEPES pH 7.4, 0.4 mM DTT for use. ... 1998), dialyzed into HKMbuffer, and centrifuged for 20 min at 100,000g immediately prior to useto remove aggregates.

7 Dec 2006: to polyvinylidene difluoride membrane for3.5 h at 20 mAmps using a wet transfer apparatus (Trans-BlotTMCell, Bio-Rad). ... Genes Dev 20: 113–124. Kunst, F., and Rapoport, G. (1995) Salt stress is an environ-mental signal affecting degradative enzyme

8 Sep 2006: of the motor domain is rotated by 20 betweenthe ADP and AMPPNP states. ... 1 mM DTT, 0.5 mM GTP, and 10% DMSO), stabilized with 20 mM.

17 Jan 2006: Here, full-length recombinant SNX2 wasincubated with liposomes comprising phosphatidylcholine(PtdCho), phosphatidylserine (PtdSer) andphosphatidylethanolamine (PtdEth) (each at 26.6% (w/w)) towhich 20% (w/w) of the relevant phosphoinositide was added

12 Nov 2006: 66). We then measured the binding of PI3K-C2α PX mutants to POPC/POPE/PtdIns(4,5)P2 (78:20:2) vesicles. ... 1996) J Biol Chem 271, 13892-13899. 20. Virbasius, J. V., Guilherme, A., and Czech, M.

4 Jan 2006: Membrane curvatureresponse. d-Amph 3-20. Amphipathic helices are stretches of -helix, one side of which is polar(charged) and the other hydrophobic. ... Proc. Natl Acad. Sci.USA 101, 8262–8269 (2004). 20. Honing, S. et al.

19 Jun 2006: Annu Rev Cell Dev Biol 20:395–425. Seeger H, Heikenwalder M, Zeller N, Kranich J, Schwarz P, Gaspert A, Seifert B, Miele G, Aguzzi A (2005) Coincident scrapie infection andnephritis

12 Nov 2006: 1 B). All the complete sequences analyzed in Fig. 1 have between 20 and 24 continuous repeats. ... 1 B). Other repeat lengths spread the corresponding amino acids at 20 intervals through the circum-ference of the helix.

12 Nov 2006: Significantly, four different tumoursuppressor genes act to suppress mTor activity (TSC1,TSC2 [19], LKB-1 [20] and VHL [21], see Figure 2). ... Trends Biochem Sci 2003, 28:573-576. 20. Shaw RJ, Bardeesy N, Manning BD, Lopez L, Kosmatka M,DePinho RA,

21 Sep 2006: 4†. (F) Cst 3 Puro 10 0.7 0.2ER-GFP (C) Steady state 20 8.7 2.5. ... H) Cst 20 5.6 1.7(E) Puro 3 Cst 8 8.5 2.3†.

19 Apr 2006: andcatastrophic (shortening results in rapid, com-plete filament disassembly with a disassemblyrate of 64 20 s1). ... When assem-bled into filaments, this should be enoughParM to form a filament 15–20 times the celllength.

31 Aug 2006: Over 20 different proteins arefound in the b2-appendage pull-down from HeLa cellextracts (Figure 1C). ... We foundthat two appendages could interact with each copy of thisdomain with affinities of around 20 lM [8].

18 Apr 2006: Avanti, 840053), 20% PE (Avanti, 830022), 10% PS (Avanti, 830032),. 10% cholesterol (Sigma, 8667) and 10% of the test phosphoinositide. ... der vacuum for 1 hr. Lipids were rehydrated in a buffer containing 20.

17 Aug 2006: Proc. Natl.Acad. Sci. U. S. A. 98, 3471–3476. 20 Lee, H.C. ... EMBO J.20, 1573–1582. 49 Chen, X. et al. (2001) Signal peptidase and oligosaccharyltransferaseinteract in a sequential and dependent manner within the endoplasmicreticulum.

14 Jun 2006: plus cytochalasin B (20 mM); circle, 1% of. input 3H-2-deoxyglucose. Error bars represent. ... IRS-1 associated. PI3-K activity increased 20- to 40-fold within 90 s of insulin.

8 Jun 2006: 20. –10. 0. 10. 20. 30. 200 210 220 230 240 250Wavelength (nm). ... 0.2. 0.4. 0.6. 0.8. 1. 1.2. 0 20 40 60 80 100Calcium concentration (µM).

30 Nov 2006: This mis-. folded PrP persisted for the ensuing 20 hr despite ER func-. ... the acute stress treatments employed in this study, recov-. ery for 16–20 hr led to substantial upregulation of BiP(and to a lesser extent PDI) due to induction of the

21 Sep 2006: 20). These findings had two major consequences for theunderstanding of prion diseases.