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21 Feb 2008: serum free medium to cells and incubate at 37 ¡C for the desired time (15-20 minutes for steady state). ... For transferrin alone, use 4¡C. Important: Fix cells with 3.2 % paraformaldehyde pre-equilibrated in serum-free medium at the required

6 Feb 2008: 1: 5 000. poly. Endophilin1. Ra 37. poly. Eps15. Santa Cruz (C-20). ... Five to 20 cells were co-plated with K. aeorogenes on SM agar plates.

11 Nov 2008: phospho IκB. IκB. YopJ-wt. TNF-α (min) 0 5 10 15 20 0 5 10 15 20. ... YopJ-C172A YopJ-wt. 0 5 10 15 20 0 5 10 15 20 TNF-α (min).

28 Jul 2008: EMBO J. 13, 5051-5061 (1994). 20. McMahon, H.T. and SŸdhof, T.C. ... Biochemical Journal 414, 189-194. Highlights of 20 papers above marked with an asterix.

15 Dec 2008: nitrogen cooling, FEI company, Eindhoven, NL) equipped with a Gatan energy filter (filter bandpass 20 eV). ... 18. Figure S4 APlasmid-free control. 19. Figure S4 BPlasmid-free control. 20.

26 Oct 2008: Prior to transfer the bag is sealed and dry nitrogen gas is flushed through it for about 20 s.

16 Jul 2008: ASCII85EncodePages false /AllowTransparency false /AutoPositionEPSFiles true /AutoRotatePages /All /Binding /Left /CalGrayProfile (Dot Gain 20%) /CalRGBProfile (sRGB IEC61966-2.1) /CalCMYKProfile (U.S.

18 Dec 2008: F) The Saci1373MIM2 (red)/Saci1372 MIT(yellow) interaction isclosely relatedin structurewith the CHMP6 MIM2(green, extended)/VPS4AMIT (gray helices) inter-action (20). ... 19. M. D. Stuchell-Brereton et al., Nature 449, 740 (2007).20. C.

30 May 2008: described)1 were incubated with or without 2 (500 nM) and 3 (0–20 µM, final. ... 20 mL) and the combined organic fractions were washed with saturated NaCl solution.

16 Oct 2008: 0 25 50 75 100. Seg1. e. 0.02. 0.2. 2. 20. ... PI-103PP121DMSO PP1. pTyr. 20 5.01. 250. 310. 08. pAkt (308). pS6pAkt (473).

4 Aug 2008: least 20 ParR C-terminal tails present, producing a very high. local concentration. ... Under these condi-. tions, rings of roughly 20 nm diameter could be observed.

18 Oct 2008: modules (19,20). The interaction with lipids has been. suggested to be mediated by electrostatic attraction. ... position (20). We note the possibility that while the. situation in PICK1 is somewhat different, the mechanism.

4 Aug 2008: different species, suggesting that each type may have specific. roles [11,12,14-20]. ... ted with outer dense fibers rather than with outer doublet. microtubules [11-20].

3 Sep 2008: Healthcare). The resin was washed with buffer C (20 mM Tris [pH 7.5],. ... 100 mM NaCl, 2 mM b-mercaptoethanol) followed by buffer D (20 mM Tris.

16 Aug 2008: R factor, R freea 0.20 (0.27), 0.25 (0.36). B factorsb 40.3 Å2, 1.15 Å2. ... Cal (UK) at 10C. Protein and DNAs were in 20 mM Tris/HCl, 1 mM EDTA,.

10 Jun 2008: elegans genomic. sequence contains at least 20 genes (designated flp- genes, for FMRFamide-like peptide. ... 20. inactive phase. Genetic analysis indicated that goa-1 probably functions to regulate egg-.

6 Mar 2008: rate of foraging [19, 20]. However, the precise nature of the foraging movements in wild-type and. ... 20. Some values are easily affected by noise or errors during image processing or capture.

17 Sep 2008: Microtubule assembly dynamics: newinsights at the nanoscale. Curr Opin Cell Biol 2008;20:64–70. ... ogliano J. The bacterial cytoskeleton. Curr Opin Cell Biol 2008;20:19–27.opp D, Narita A, Oda T, Fujisawa T, Matsuo H, Nitanai Y, et al.

13 Dec 2008: C2 domain-containing proteins, such as synaptotagmins (17),. and proteins of the reticulon and DP1/Yop1 families (20,21). ... flat membrane into tubules of ;20 nm diameter (11). SmallG-proteins Arf1 and Sar1 expose amphipathic a-helices uponexchange of

30 May 2008: clustering in three distinct domains: a) nine functional PT sequences appear in the amino terminal 20% of apoB, b) four more PT sequences occur just before the end of apoB48, and ... Conversely, two large regions, apoB- 20-44 and apoB-47-67, each