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8 Dec 2007: However, K338Aand R349A showed 3- and 4-fold reduced affinity, respectively,for more anionic POPC/POPE/POPS/PtdIns(4)P (57:20:20:3)vesicles. ... 3)P (77:20:3) (f), POPC/POPE/PtdIns(5)P (77:20:3) (Œ),and POPC/POPE/POPS (60:20:20) (") monolayers.

25 Jun 2007: Mix by inversion to ensure homogeneity. 11. Spin in a 70Ti rotor at 25000rpm for 20 minutes. ... Run at 1ml/min (Abs. 0.5) and collect 2ml fractions. Clathrin elutes at around fraction 20.

25 Jun 2007: Crystals were equilibrated in 20% glycerol for cooling to 100K. The crystal asymmetric unit contains one molecule, and the crystals belong to spacegroup P3121, cell dimensions a = b = 49.6Å, c = ... Sedimentation was at 11,000 rev/min, 20.0C, with

25 Jun 2007: NB: the filtration process usually leads to loss of around 100-200 ul of liquid, and 10-20% of the lipid in the reamining volume.

25 Jun 2007: EpsinR L10E overexpression in COS cells. Myc-EpsinR L10E with endogenous AP1 and internalised transferrin in COS cells. Insertion of a glutamic acid (E) in place of the hydrophobic leucine (L) is predicted to affect epsinR's ability to bend membranes

25 Jun 2007: Purified clathrin. Clathrin should be dialyzed into HKM buffer and centrifuged for 20 minutes at 100000 gmax (e.g., 45000 r.p.m.

25 Jun 2007: Usually I mix at least 20 ul; otherwise evaporation can drastically change the salt content. ... Pipette drops of UrAc (20-40 ul each) onto ParafilmÑtwo drops per sample.

25 Jun 2007: Current Biol. Oct 26;14(20):1791-800. (see also Research Roundup? Snx1 hugs the curves?

2 Apr 2007: Purified tubulins were desalted into PEM containing 20 μM GDP before storage in liquid nitrogen. ... Enzyme reactions were done in 20 mM PIPES pH 6.9, 2 mM MgCl2, using quartz fluorimeter cuvettes.

6 Apr 2007: 0 5 10 15 20. 0 5 10 15 20. 0 5 10 15 20-10. ... Themicrotubule plus end is toward the top of thepage. (Left) Cryo-EM map (20).

2 Jun 2007: Samples at different. loading concentrations were sedimented at 10,000 rpm at 20.0 ºC in double sector cells.

25 Jul 2007: 18:30 Dinner 20:00 Opening event: Concert at Schloß Waldthausen by „Trio con brio“ (piano trio) 22:00 Get together in the „Weinkeller“ of Schloß Waldthausen Thursday, Oct 11th, 2007

8 Dec 2007: Concentration (µM)0 20 40 60 80 100. I–I 0. (arb. itrar. ... interaction. Indeed, CHMP1Bbinds human Vps4A MIT domain with a Kd of 20 mM (ref.

14 Jun 2007: Chem. 279, 36059–36071. 20. Bache, K.G., Slagsvold, T., Cabezas, A., Rosendal, K.R., Raiborg, C. ... and Sundquist, W.I. (2004) Annu. Rev. Cell Dev. Biol. 20, 395–42553.

8 Dec 2007: 0. 20. 40. 60. 80. 100. 120. 140. 0 50 100 150p85ni (nM). ... 65, 4562 (2005).20. S. J. Isakoff et al., Cancer Res. 65, 10992 (2005).21.

15 Oct 2007: and then incubated with pig brain dynactin complex at 30 1Cfor a further 20 min. ... 20 mg/ml) and 15% v/v heat-inactivated fetal calf serum (Invitrogen,Paisley, UK).

23 Jul 2007: 40. 80. 120. 0 20 40 60. Req. (R. U). PX concentration (nM). ... layer to the level of its penetration into POPC/POPE (80:20). monolayer.

31 Oct 2007: Apart from the movement of the necklinker, the most striking difference between the ADP and ATP-like states, a 20 rotation of the core domain relative to 4, isalso evident in ... EMBO J. 20, 2611-2618. Yun, M., Bronner, C. E., Park, C.

4 Oct 2007: 2007 European Molecular Biology Organization The EMBO Journal VOL 26 | NO 20 | 2007. ... 2007 European Molecular Biology Organization The EMBO Journal VOL 26 | NO 20 | 2007 4415.

2 Jun 2007: mM NaCl, 20 mM HEPES [pH 7.5], 2.5 mM DTT), and the GST tag. ... for 30 min at room temperature in Beckman 7 3 20 mm polycarbonate.