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28 Sep 2007: 3,000. 0. 20. 40. 60. 80. 100. 180160140120100806040200 200Time (min). PtdIns(4)P. ... The. column was extensively washed with 20 mM HEPES (pH 7.5), 700 mM NaCl,.

2 Jun 2007: mM NaCl, 20 mM HEPES [pH 7.5], 2.5 mM DTT), and the GST tag. ... for 30 min at room temperature in Beckman 7 3 20 mm polycarbonate.

24 Sep 2007: º) 90, 106.5, 90. Wavelength 0.97962. Resolution (Å) 20 – 3.1 (3.2 – 3.1). ... www.nature.com/nature 20. Supplementary notes / Supplementary References. 23. Lenzen, C., Cool, R.

15 Feb 2007: selecting flow from a multichambered valve-controlledmechanical fed reservoir. Excitation light was provided bya 20-mW Argon Krypton laser. ... Each analog plot is representative of atleast three experiments, with each trace showing the average for

11 May 2007: If using antibodies to label the protein(s) ofinterest, either monoclonal or mono-specificpolyclonal antibodies (raised against shortpeptide sequences of 8 to 20 amino acids)are preferable to broad specificity ... While the overall trends and

15 Aug 2007: For fractionation, cytosolic proteins were selectively extracted on icefor 5 min with 1 ml/35-mm dish of 100 g/ml digitonin in KHM buffer (110 mMKAc, 20 mM HEPES, pH ... Cross-linker was quenched with 20 mM Tris-Cl, pH 7.5, the cells were lysed in1% SDS

15 May 2007: 14,19,20). The IVF motif also contributes to the intra-. molecular interaction but with significant difference among.