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28 Sep 2009: This article was published as part of the. 2009 Green Fluorescent Protein issue. Reviewing the latest developments in the science of green fluorescent protein. Guest Editors Dr Sophie Jackson and Professor Jeremy Sanders. All authors contributed to

1 Aug 2008: several orders of magnitude smaller (typi-. cally 1–20 nanometers). They do this, in.

11 Sep 2003: 20 1.11( 0.16 -2.72( 0.32WL59 5.35( 0.05 1.20( 0.08 -2.35( 0.09. ... 20. Kraulis, P. (1991)J. Appl. Crystallogr. 24, 946-950.21. Laitinen, P. J., Brown, K.

16 Sep 2003: 60. 70. 80. 10 20 30 40 50 60 70 80 90 100 110. ... 20. Zweifel ME, Barrick D: Studies of the ankyrin repeats of theDrosophila melanogaster Notch receptor.

6 Jan 2005: Science 292, 2281–2285 (2001).20. Queitsch, C., Sangster, T.A. & Lindquist, S. ... 458, 117–123. (1999). 20. 04 N. atur. e P. ublis. hing.

13 Sep 2006: 20. 0. 20. 40. 60. 80 60 40 20 0 20 40 60. ... 40. 20. 0. 20. 40. eigenvector 1 (Å). ed along their first two principal eigenvectors: (a) ubiquitin,.

5 Dec 2012: Lys 48-linked diUb. Catalyticcentre. b OTUB1i titration. 20 40 60 80 100–0.4. ... 20 nM USP21i86. 24. 0 0.2 0.4 0.6 0.8 1.0FRET efficiency.

17 Jul 2005: 20. 0. 20. 40. 60. 80 60 40 20 0 20 40 60. ... 40. 20. 0. 20. 40. eigenvector 1 (Å). ed along their first two principal eigenvectors: (a) ubiquitin,.

16 Sep 2003: Englander, S.W. Nature Struct. Biol. 5, 882–884 (1998).20. Dabora, J.M. & ... Wemmer, D.E. Biopolymers 32, 1695–1702 (1992).20. Luidens, M.K., Figge, J., Breese, K. &

17 Jul 2005: 29 2.20 0.10 0.010 0.005 2.34 0.11 0.5 0.4. ... Regan, L. (2003). Structure (London) 11, 1–20.7. Main, E. R., Fulton, K.

15 Sep 2003: For example, the folding of amonomeric form of theλ repressor has been studied by NMRline-shape analysis19 and fluorescence stopped-flow spectros-copy.20 Together with results from protein engineering and ... 98, 7760-7764.(19) Huang, G. S.; Oas, T. G.

17 Sep 2003: O1)Cδ2 11 Leu 103 (Cδ1), Pro 16 (Cγ, CR, Câ), Gln 20. ... S. W., and Holzman, T. F. (1993)Biochemistry 32, 1920-1927. 20. Michnick, S.

8 Dec 2008: AdoHcy, implyingthat the cofactor binding site, and therefore the knotted regionof the protein, becomes only completely structured in thedimeric state of the protein (20). ... 16, 20, and 23. Analysis of urea denaturation profiles was performedas in ref.

23 Aug 2004: amide groups [20]. The presence of intermediate states is often inferred from. ... and Clarke, A.R. (1997). Biochemistry 36, 13396–13405.[20] Gladwin, S.T. and Evans, P.A.

15 Sep 2003: DNA by DNA gyr-ase B.20 All evidence so far suggests that p23 bindsto this state, thereby stabilising the heterocomplexand, for human Hsp90, inhibiting ATP hydrolysis. ... Biol. Chem. 275, 18054-18060. 20. Wigley, D. B., Davies, G. J., Dodson, E.

26 Aug 2005: b-galactoside [51]. 1KRV [51]. LRR A 20- to 29-residue motif forming a. ... An interestingextension of this work was to select for ANK variants thatfunction, both in vitro and in vivo, as kinase inhibitors[20].

10 May 2010: This result can explain the efficient folding reactionof YibK and YbeA observed in vitro (20, 26) and has important. ... Proc Natl Acad Sci USA 106:3119–3124. 20. Mallam AL, Jackson SE (2005) Folding studies on a knotted protein.

14 Nov 2011: Favorable binding was howeverobserved after prior binding of Hop to the Hsp90 dimer (KD 20 nM). ... MethodsProtein Purification. Hsp90 and cochaperones were human, expressed inEscherichia coli and purified as previously described (20, 29).

8 Feb 2008: A 200 μl sampleof 20 μM DMAG was injected into a 1.4 ml cell containing1 μM wild-type Hsp90. ... Edited by S. Radford. (Received 17 April 2007; received in revised form 20 June 2007; accepted 22 June 2007)Available online 29 June 2007.

3 Apr 2009: ubiquitin in living cells [20]. Recently, certain UCHs. have also been assigned an ubiquitin ligase function. ... cally using an extinction coefficient at 280 nm of. 20 065 m)1Æcm)1.