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The extremely slow-exchanging core andacid-denatured state of green…
www-jackson.ch.cam.ac.uk/publications/2008/2008_HFSP_J_GFP.pdf19 Dec 2008: Denaturedsamples at pH 2.9 were left in D2O for 5, 20, 40, or 80 minand then refolded and 15N, 1H correlation spectra of the na-tive state acquired. ... Protein stabilityparameters measured by hydrogen exchange.” Proteins 20, 4–14. Bai, Y, Sosnick, TR -
Folding Pathway of FKBP12 and Characterisation of the Transition State
www-jackson.ch.cam.ac.uk/publications/1999/1999_JMB2.pdf15 Sep 2003: open triangles).(b) Guandinium chloride-induced unfolding of wild-type FKBP12 in the absence (lled circles) and presenceof 20 mM rapamycin (open circles). ... Comparison with other proteins. There are now at least 20 proteins which havebeen reported to -
doi:10.1016/j.molcel.2008.03.019
www-jackson.ch.cam.ac.uk/publications/2008/2008_Mol_Cell_Knotted_Fusions.pdf1 Aug 2008: commented on the manuscript. Received: November 20, 2007. Revised: February 22, 2008. -
doi:10.1016/j.str.2006.11.007
www-jackson.ch.cam.ac.uk/publications/2007/2007_structure_YibK.pdf8 Feb 2008: present. as dimer at 1 mM protein. 100 90 20 1 99 96 2 0. ... mM DTT. (B) YibK mutant denaturation profiles for 100. (pink), 50 (dark purple), 20 (light purple), 10. -
Evidence of an Intermediate and Parallel Pathways in ProteinUnfolding …
www-jackson.ch.cam.ac.uk/publications/2008/2008_JACS_SM_GFP.pdf1 Aug 2008: Im9,20 adenylate kinase,21 and protein A22 under equilibriumconditions. All these studies showed typical two-state-foldingbehavior, and no intermediate states were detected. ... The only reactive Cys residue is the mutation. (20) Tezuka-Kawakami, T.; Gell -
Protein folding: Defining a “standard” set ofexperimental conditions…
www-jackson.ch.cam.ac.uk/publications/2005/2005_Maxwell_ProSci_proteinfolding.pdf19 Apr 2005: Temperature. Protein folding rates display a strong dependence on tem-perature. Folding reactions commonly exhibit activation en-thalpies of 10–20 kJ/mol (e.g., Jackson and Fersht 1991b;Plaxco et ... 56 5.70 0.18 4.20 0.25 32.5 1.4 8.3 0.3 -
doi:10.1016/j.jmb.2008.02.013
www-jackson.ch.cam.ac.uk/publications/2008/2008_JMB_Hsp90_Hop.pdf4 Jun 2008: 20 SAXSmeasurements of full-length and truncated Hop. Fig. 5. (a) Solution structure of Cyp40. ... 6 mM MgCl2, 20 mM KCl and 1 mM tris(2-carboxyethyl)phosphine at 25 C. -
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www-jackson.ch.cam.ac.uk/publications/2004/2004_Biochemistry_UBQ_core.pdf23 Aug 2004: Bufferswere prepared volumetrically. All stock GdmCl solutionswere prepared in volumetric flasks and stored in aliquots at-20 C until use. ... In comparison, other studies haveshown that single point mutations (V26I or V26L) can resultin an increased -
doi:10.1016/j.jmb.2005.11.085
www-jackson.ch.cam.ac.uk/publications/2006/2006_McLaughlin_JMB_Hsp90.pdf21 Apr 2006: The reduction in thechange in resonance reflects a weaker interactionwith an approximately 20-fold increase in thedissociation constant (33 mM) at 25 8C. ... The major. e spectra were typically acquired at a capillary voltage ofon of 20 mM. -
doi:10.1016/j.jmb.2006.11.014
www-jackson.ch.cam.ac.uk/publications/2007/2007_J_mol_biol_YbeA+YibK.pdf8 Feb 2008: 2.460.01 5.280.01 20.20.110 2.530.01 5.400.1 20.50.420 - - -. Fluorescence was monitored at 350 nm. Parameters are quoted with thto a ... 0.01. 2.50.01. 1.38. 0.01. 20.20.1. 4.40.02. Ybe. ANon. eFL. 0.75. 0.1.
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