Search

28 Jul 2012: O2A PA O3A PB 60.005 20.000 1 ADP var_2 PA O3A PB O1B 59.979 20.000 1 ADP var_3 O2A PA "O5'" "C5'" -59.942 ... O4'" "C1'" 150.000 20.000 1 ADP var_7 "C5'" "C4'" "C3'" "C2'" -150.000 20.000 3.

15 Mar 2012: 5 µM) in buffer BC containing the indicated nucleotides (1 mM) and no (F) or 20 mM (G) EDTA. ... Crystallization was observed in three buffers, which all contained ATPγS and 100 mM Tris (pH 8.5) with 18% - 20% ethanol.

7 Dec 2012: Cell 12, 1477 (2003).20. J. Salje, B. Zuber, J. Löwe, Science 323, 509 (2009);. ... 20 August 2012; accepted 12 October 2012Published online 25 October 2012;10.1126/science.1229091.

23 Jan 2012: n d for a s e c o n d image a t 20,000 A u n - derfocus. ... and the second one at ,20,000 A underfocus, using a nominal magnification of 36,000.

28 Sep 2012: 244.20 Secondary Structure Matching (SSM). 244.21 Least-Squares Fitting. 254.22 Ligand Overlaying. ... 536.18 Trimming. 536.19 Map Transformation. 536.20 Export Map. 54. 7 Validation.

1 Nov 2012: We used 20-30 rats (Sprague-Dawley, Madison, WI), of 160 g body wt, for each set of experiments. ... pieces, and homogenized in 20-ml portions in a Potter-Elvehjem glass homogenizer with a loose-fitting teflon pestle.

21 Nov 2012: hIST1 and CHMP1B recruit the AAA-ATPaseSpastin, which severs the microtubule bundle spanning thelength of the intercellular bridge, an activity essential for ab-scission (20, 21). ... Science321(5894):1354–1357. 20. Connell JW, Lindon C, Luzio JP, Reid

21 Nov 2012: 2. 2. 2. 210. 10. 20. 20. Fc1: PC/PE/cholesterol. ... Res. pons. e un. it PC/PE/cholesterol/20% PS/2% PIP2. -. p110/p85α. α/nic.

25 Nov 2012: It has been proposed that mutations in the ABDdomain could disrupt interactions with the rest of the catalyticsubunit (12, 20); however, no clear mechanism by which thisconformational shift would activate lipid ... Proc Natl Acad Sci USA 104:5569–5574.

23 Oct 2012: crystallization. Crystals were obtained in 20 % PEG 6000, 100 mM Bicine pH 8.6, 1 M LiCl, at 10 mg/ml. ... Isothermal Calorimetry (ITC). ITC experiments were performed using a MicroCal iTC200 machine with a ParM concentration of 20 µM and.

12 Oct 2012: ring-like structures, with a typical diameter of 15–20 nm, mean 17.5, n = 43 (Fig. ... Nature 450:1268–1271. 20. Dam M, Gerdes K (1994) Partitioning of plasmid R1.

5 Dec 2012: The mixture was loaded onto a col-umn cartridge and extensively washed with buffer containing 20 mM im-idazole. ... buffer containing 20 mM Hepes-NaOH (pH 7.7), 100 mM NaCl, and 2 mM TCEP.

27 Mar 2012: The divisome is currently known to. contain more than 20 components and can be divided into. ... Next, the tubes were centri-fuged at 57 k at 20 1C for 30 min.

17 Jan 2012: Received: Dec 2, 2010Revised: Aug 16, 2011Accepted: Sep 20, 2011. These authors contributed equally to this work.The authors declare no competing interests.Address correspondence to: Richard Lundmark (Richard.lundmark@medchem. ... Louis, MO) and used at

23 May 2012: 293T cells expressing. YFP-tagged TCRa were treated with MG132 (20 mM) for 4 hr. ... tions specified in the figure legends in a buffer containing 20 mM HEPES 7.4,.

12 Oct 2012: Cells were fixed in4% paraformaldehyde for 20 minutes at room temperature and stained forTRAP. ... Scale bars,200 nm in panel A and 20 m in panels B through D.

7 Mar 2012: 20) Page 12, line 9-10: Assuming that cell filamentation is worse with the FtsA mutants and assuming that these mutants really are worse at polymerizing than WT FtsA, increased cell ... 20. Page 12, line 9-10: Assuming that cell filamentation is worse