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11 Oct 2007: The nucleotide content was measured asdescribed.20. Untagged, full-length ftsZ from B. ... J. Biol. Chem.278, 37664–37671. 20. Oliva, M. A., Huecas, S., Palacios, J.

22 Aug 2007: Such attachments can be used in manydiverse pathways (for example, actin polymerization associated withvesicle scission and motility18–20). ... Nature Cell Biol. 4,691–698 (2002). 20. Kaksonen, M., Toret, C. P. &

22 Aug 2007: These data come from the following papers: (AP2, clathrin)1,20, (dynamin, clathrin)21,22,. ... endocytosis in Drosophila. Genes Dev. 15, 2967-2979 (2001). 20. Rappoport, J.

14 Jun 2007: of clustered ubiquitinated cargo before cargo sorting intoReceived: 22 August 2006; accepted: 20 November 2006; publishedonline: 11 January 2007. ... 0.19. 0.20. 0.21. 0.23. 0.24. 0.25. 0.26. Ani. sotr. opy. Concentration (nM).

21 Aug 2007: to contain up to 20 different component in appropriate stoichiometries that ensure efficient targeting, fusion and recycling/refilling of endocytosed vesicles. ... over 20 different proteins bind to the AP2 a-appendage). When we plot proteins known to be

22 Jul 2007: Thepurification procedure of the complex was the same as for the scFvdescribed in Supplementary data, except the extraction buffer(25 mM Na phosphate, pH 7.4, 300 mM NaCl, 20 mM ... imidazole)and gel filtration buffer (20 mM Tris–HCl pH 8.0 at

15 Feb 2007: selecting flow from a multichambered valve-controlledmechanical fed reservoir. Excitation light was provided bya 20-mW Argon Krypton laser. ... Each analog plot is representative of atleast three experiments, with each trace showing the average for

28 Sep 2007: 3,000. 0. 20. 40. 60. 80. 100. 180160140120100806040200 200Time (min). PtdIns(4)P. ... The. column was extensively washed with 20 mM HEPES (pH 7.5), 700 mM NaCl,.

24 Sep 2007: º) 90, 106.5, 90. Wavelength 0.97962. Resolution (Å) 20 – 3.1 (3.2 – 3.1). ... www.nature.com/nature 20. Supplementary notes / Supplementary References. 23. Lenzen, C., Cool, R.

14 Jun 2007: Recruitment of ESCRT-0 to the endosomal membrane. The N-terminal FYVE domain of HRS mediates recog-nition of PtdIns3P, a highly abundant phosphoinositide in endosomal membranes18–20. ... Clathrin acts as an organizer of the ESCRT pathway. HRS is highly

15 Aug 2007: For fractionation, cytosolic proteins were selectively extracted on icefor 5 min with 1 ml/35-mm dish of 100 g/ml digitonin in KHM buffer (110 mMKAc, 20 mM HEPES, pH ... Cross-linker was quenched with 20 mM Tris-Cl, pH 7.5, the cells were lysed in1% SDS

11 May 2007: If using antibodies to label the protein(s) ofinterest, either monoclonal or mono-specificpolyclonal antibodies (raised against shortpeptide sequences of 8 to 20 amino acids)are preferable to broad specificity ... While the overall trends and

15 May 2007: 14,19,20). The IVF motif also contributes to the intra-. molecular interaction but with significant difference among.