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8 Dec 2007: However, K338Aand R349A showed 3- and 4-fold reduced affinity, respectively,for more anionic POPC/POPE/POPS/PtdIns(4)P (57:20:20:3)vesicles. ... 3)P (77:20:3) (f), POPC/POPE/PtdIns(5)P (77:20:3) (Œ),and POPC/POPE/POPS (60:20:20) (") monolayers.

2 Apr 2007: Purified tubulins were desalted into PEM containing 20 μM GDP before storage in liquid nitrogen. ... Enzyme reactions were done in 20 mM PIPES pH 6.9, 2 mM MgCl2, using quartz fluorimeter cuvettes.

6 Apr 2007: 0 5 10 15 20. 0 5 10 15 20. 0 5 10 15 20-10. ... Themicrotubule plus end is toward the top of thepage. (Left) Cryo-EM map (20).

2 Jun 2007: Samples at different. loading concentrations were sedimented at 10,000 rpm at 20.0 ºC in double sector cells.

25 Jul 2007: 18:30 Dinner 20:00 Opening event: Concert at Schloß Waldthausen by „Trio con brio“ (piano trio) 22:00 Get together in the „Weinkeller“ of Schloß Waldthausen Thursday, Oct 11th, 2007

8 Dec 2007: Concentration (µM)0 20 40 60 80 100. I–I 0. (arb. itrar. ... interaction. Indeed, CHMP1Bbinds human Vps4A MIT domain with a Kd of 20 mM (ref.

8 Dec 2007: 0. 20. 40. 60. 80. 100. 120. 140. 0 50 100 150p85ni (nM). ... 65, 4562 (2005).20. S. J. Isakoff et al., Cancer Res. 65, 10992 (2005).21.

14 Jun 2007: Chem. 279, 36059–36071. 20. Bache, K.G., Slagsvold, T., Cabezas, A., Rosendal, K.R., Raiborg, C. ... and Sundquist, W.I. (2004) Annu. Rev. Cell Dev. Biol. 20, 395–42553.

15 Oct 2007: and then incubated with pig brain dynactin complex at 30 1Cfor a further 20 min. ... 20 mg/ml) and 15% v/v heat-inactivated fetal calf serum (Invitrogen,Paisley, UK).

31 Oct 2007: Apart from the movement of the necklinker, the most striking difference between the ADP and ATP-like states, a 20 rotation of the core domain relative to 4, isalso evident in ... EMBO J. 20, 2611-2618. Yun, M., Bronner, C. E., Park, C.

23 Jul 2007: 40. 80. 120. 0 20 40 60. Req. (R. U). PX concentration (nM). ... layer to the level of its penetration into POPC/POPE (80:20). monolayer.

4 Oct 2007: 2007 European Molecular Biology Organization The EMBO Journal VOL 26 | NO 20 | 2007. ... 2007 European Molecular Biology Organization The EMBO Journal VOL 26 | NO 20 | 2007 4415.

22 Aug 2007: Such attachments can be used in manydiverse pathways (for example, actin polymerization associated withvesicle scission and motility18–20). ... Nature Cell Biol. 4,691–698 (2002). 20. Kaksonen, M., Toret, C. P. &

22 Aug 2007: These data come from the following papers: (AP2, clathrin)1,20, (dynamin, clathrin)21,22,. ... endocytosis in Drosophila. Genes Dev. 15, 2967-2979 (2001). 20. Rappoport, J.

11 Oct 2007: The nucleotide content was measured asdescribed.20. Untagged, full-length ftsZ from B. ... J. Biol. Chem.278, 37664–37671. 20. Oliva, M. A., Huecas, S., Palacios, J.

2 Jun 2007: mM NaCl, 20 mM HEPES [pH 7.5], 2.5 mM DTT), and the GST tag. ... for 30 min at room temperature in Beckman 7 3 20 mm polycarbonate.

14 Jun 2007: of clustered ubiquitinated cargo before cargo sorting intoReceived: 22 August 2006; accepted: 20 November 2006; publishedonline: 11 January 2007. ... 0.19. 0.20. 0.21. 0.23. 0.24. 0.25. 0.26. Ani. sotr. opy. Concentration (nM).

22 Jul 2007: Thepurification procedure of the complex was the same as for the scFvdescribed in Supplementary data, except the extraction buffer(25 mM Na phosphate, pH 7.4, 300 mM NaCl, 20 mM ... imidazole)and gel filtration buffer (20 mM Tris–HCl pH 8.0 at

15 Feb 2007: selecting flow from a multichambered valve-controlledmechanical fed reservoir. Excitation light was provided bya 20-mW Argon Krypton laser. ... Each analog plot is representative of atleast three experiments, with each trace showing the average for

28 Sep 2007: 3,000. 0. 20. 40. 60. 80. 100. 180160140120100806040200 200Time (min). PtdIns(4)P. ... The. column was extensively washed with 20 mM HEPES (pH 7.5), 700 mM NaCl,.