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26 Jan 2006: Dialyze into 150 mM NaCl, 20 mMHEPES pH 7.4, 0.4 mM DTT for use. ... 1998), dialyzed into HKMbuffer, and centrifuged for 20 min at 100,000g immediately prior to useto remove aggregates.

7 Dec 2006: to polyvinylidene difluoride membrane for3.5 h at 20 mAmps using a wet transfer apparatus (Trans-BlotTMCell, Bio-Rad). ... Genes Dev 20: 113–124. Kunst, F., and Rapoport, G. (1995) Salt stress is an environ-mental signal affecting degradative enzyme

8 Sep 2006: of the motor domain is rotated by 20 betweenthe ADP and AMPPNP states. ... 1 mM DTT, 0.5 mM GTP, and 10% DMSO), stabilized with 20 mM.

17 Jan 2006: Here, full-length recombinant SNX2 wasincubated with liposomes comprising phosphatidylcholine(PtdCho), phosphatidylserine (PtdSer) andphosphatidylethanolamine (PtdEth) (each at 26.6% (w/w)) towhich 20% (w/w) of the relevant phosphoinositide was added

12 Nov 2006: 66). We then measured the binding of PI3K-C2α PX mutants to POPC/POPE/PtdIns(4,5)P2 (78:20:2) vesicles. ... 1996) J Biol Chem 271, 13892-13899. 20. Virbasius, J. V., Guilherme, A., and Czech, M.

4 Jan 2006: Membrane curvatureresponse. d-Amph 3-20. Amphipathic helices are stretches of -helix, one side of which is polar(charged) and the other hydrophobic. ... Proc. Natl Acad. Sci.USA 101, 8262–8269 (2004). 20. Honing, S. et al.

19 Jun 2006: Annu Rev Cell Dev Biol 20:395–425. Seeger H, Heikenwalder M, Zeller N, Kranich J, Schwarz P, Gaspert A, Seifert B, Miele G, Aguzzi A (2005) Coincident scrapie infection andnephritis

12 Nov 2006: 1 B). All the complete sequences analyzed in Fig. 1 have between 20 and 24 continuous repeats. ... 1 B). Other repeat lengths spread the corresponding amino acids at 20 intervals through the circum-ference of the helix.

12 Nov 2006: Significantly, four different tumoursuppressor genes act to suppress mTor activity (TSC1,TSC2 [19], LKB-1 [20] and VHL [21], see Figure 2). ... Trends Biochem Sci 2003, 28:573-576. 20. Shaw RJ, Bardeesy N, Manning BD, Lopez L, Kosmatka M,DePinho RA,

21 Sep 2006: 4†. (F) Cst 3 Puro 10 0.7 0.2ER-GFP (C) Steady state 20 8.7 2.5. ... H) Cst 20 5.6 1.7(E) Puro 3 Cst 8 8.5 2.3†.

19 Apr 2006: andcatastrophic (shortening results in rapid, com-plete filament disassembly with a disassemblyrate of 64 20 s1). ... When assem-bled into filaments, this should be enoughParM to form a filament 15–20 times the celllength.

31 Aug 2006: Over 20 different proteins arefound in the b2-appendage pull-down from HeLa cellextracts (Figure 1C). ... We foundthat two appendages could interact with each copy of thisdomain with affinities of around 20 lM [8].

18 Apr 2006: Avanti, 840053), 20% PE (Avanti, 830022), 10% PS (Avanti, 830032),. 10% cholesterol (Sigma, 8667) and 10% of the test phosphoinositide. ... der vacuum for 1 hr. Lipids were rehydrated in a buffer containing 20.

17 Aug 2006: Proc. Natl.Acad. Sci. U. S. A. 98, 3471–3476. 20 Lee, H.C. ... EMBO J.20, 1573–1582. 49 Chen, X. et al. (2001) Signal peptidase and oligosaccharyltransferaseinteract in a sequential and dependent manner within the endoplasmicreticulum.

14 Jun 2006: plus cytochalasin B (20 mM); circle, 1% of. input 3H-2-deoxyglucose. Error bars represent. ... IRS-1 associated. PI3-K activity increased 20- to 40-fold within 90 s of insulin.

8 Jun 2006: 20. –10. 0. 10. 20. 30. 200 210 220 230 240 250Wavelength (nm). ... 0.2. 0.4. 0.6. 0.8. 1. 1.2. 0 20 40 60 80 100Calcium concentration (µM).

30 Nov 2006: This mis-. folded PrP persisted for the ensuing 20 hr despite ER func-. ... the acute stress treatments employed in this study, recov-. ery for 16–20 hr led to substantial upregulation of BiP(and to a lesser extent PDI) due to induction of the

21 Sep 2006: 20). These findings had two major consequences for theunderstanding of prion diseases.