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Organization of spines on the dendritesof Purkinje cellsJohn O’Brien…
https://www2.mrc-lmb.cam.ac.uk/groups/nu/pdf/pnas06.pdf25 Jul 2006: The fish dendrites are straighter and less branched than theothers, but some weaver dendrites are also straight over lengths of 20 –30! ... mM NaCl"10 mM KCl"2 mM CaCl2"1 mMMgCl2"2 mM NaHCO3"4 mM Hepes, pH 7.4, supplementedwith Medium 199 (Sigma) in a -
15N labeling
https://www2.mrc-lmb.cam.ac.uk/groups/JYL/methods/15N%20labeling.pdf28 Mar 2006: Add to M9 prior to experiment (per litre):15NH4Cl 1g (can dissolve in 10 mL H2O and filter sterilise into media)20% glucose 20 mLvitamins 1 mLtrace elements 10 mLMgSO4 (1M) ... 10 mL of 20% arabinose for BAI cells). Purification:As normal, however -
HiroseSuppl
https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/supp_MC2006.pdf25 Aug 2006: The thickness of the. sections is 15 Å in (A-F), and 20 Å in (G-I). ... was reported to rotate clockwise by 20 in the ADP state compared with the AMPPNP state. -
FigS1
https://www2.mrc-lmb.cam.ac.uk/groups/hmm/publica/SchmidBetaPaper/BetaHubSup.pdf7 Sep 2006: 15%out of 132 residues. 22%out of 114 residues. 20%out of 237. ... residues. 20%out of 128 residues. 128 residues in total. hβ4-appendage. 237 residues in total. -
Supplementary Figure 1Lysophosphatidic acid acyl transferase (LPAAT)…
https://www2.mrc-lmb.cam.ac.uk/groups/hmm/publica/GallopEndoSupFigs.pdf4 Jan 2006: ratio. n (D. 280). 0 10 20. 0.00. 0.05. 0.10. 0.15. ... n (D. 280). 0 5 10. 0.00. 0.10. 0.20. s (Svedbergs). -
LETTERS A bacterial dynamin-like proteinHarry H. Low1 & Jan ...
https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/nature%20BDLP%202006.pdf28 Nov 2006: 100 120. kDa 220. Nosto. c p. ly. sate. M. 0 0 0.2 0.4 0.6 0.8 1.0 1.2 40 80 60 20. ... 60. 40. 0. 20. 80. WT K82A mutant. 0.2 0.4 0.6 0.8 1.0 1.2. -
� Supplementary Figure 1 Bacterial dynamin-like protein (BDLP) from…
https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/nature%20BDLP%202006%20sup.pdf28 Sep 2006: The lysate buffer consisted of 50 mM Tris-Cl, 1.25 M sodium chloride, final pH 8.0, the wash buffer of 50 mM Tris-Cl, 500 mM sodium chloride, 20% ... For the phylogenetic tree, 10-20 sequences related to the different classes of dynamin-related proteins -
doi:10.1016/j.jbbm.2005.12.008
https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/rfcloning2006%20published.pdf19 Apr 2006: 2.2. Linear amplification reaction. 2–20 nM of PCR product was used in a linear amplification reaction with 6–400 pM ofpHis17 (B. ... The protein was purified over a Ni-NTA spin column under native conditions (lanes 3 and 5) and separated on a 10–20 -
Supplementary Figure 1 EPR spectra of R1-labeled endophilin A1 ...
https://www2.mrc-lmb.cam.ac.uk/groups/hmm/publica/GallopEndophilinEMBO/EndoSupFigures.pdf8 Jun 2006: Readings were performed at 20 C using an average of 5 scans with buffer subtracted. ... uals. 6.00 10 20Concentration (µM). 0. 20. 40. 60. 80 (k. -
© 2005 Nature Publishing Group Endophilin and CtBP/BARS are ...
https://www2.mrc-lmb.cam.ac.uk/groups/hmm/publica/GallopEndopohilinNature2005.pdf4 Jan 2006: fatty acid-free BSA and 0.01% Triton X-100 with substrate concentrations (20–200 mM LPA) at 25 8C and 37 8C with 14C-labelled oleoyl-CoA (Amersham) at aspecific ... Liposomes made from Folch brain lipid extract at0.2 mg ml21 in 20 mM HEPES pH 7.4, 150
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