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3 Apr 2009: ubiquitin in living cells [20]. Recently, certain UCHs. have also been assigned an ubiquitin ligase function. ... cally using an extinction coefficient at 280 nm of. 20 065 m)1Æcm)1.

17 Sep 2003: Fig. 3. Far-UV circular dichroism spectra. Refolded GR-LBD in 20 mMphosphate pH 8.0, 150 mM NaCl, 0.1% Zwittergent 3–12 at 25C (filledcircles) and 95C (open circles) ... C) Far-UV CD ellipticity at 222 nm as a function of denaturant concentration at 3

26 Jul 2010: affinity for small molecules, at only 1.5–20 nL optical sample volumes without the need for. ... 12, 20 lM rapamycin, 50 mM Tris.HCl at pH 7.5, 1 mM DTT, 25C.40.

16 Sep 2003: The transition state has been characterisedusing three different experimental and computational approaches which are in goodagreement [17, 20]. ... 2. Kinetic investigations. Biochemistry 26 (1989), 691–699. 20. Fulton, K.F., Main, E.R.G., Daggett, V.

25 Aug 2006: the size of GFP at 10-20 T, thetransverse19F relaxation is dominated by the CSA term and isvery fast. ... The greenfractions were pooled and concentrated (Vivaspins 20, Vivascience)and washed through with PBS.

16 Nov 2006: ce. 120. 100. 80. 60. 40. 20. 0. 0 1 2 3 4 5. ... Understanding, improving and using green fluorescent proteins. Trends Biochem. Sci. 20, 448–455 (1995).

13 Jan 2012: Protein folding on the. ribosome. Curr. Opin. Struct. Biol. 20, 33–45 (2010).23. ... Anal. Biochem. 387, 20–29 (2009). acknowledgmentsWe thank E. O’Brien, D. Hsu, F.

8 Feb 2008: 6250 3.540.02 2.30.1 7.60.4 1.50.6300 3.710.01 3.20.2 7.90.4 1.80.6. ... Unlike in other experiments [30,62,20], the rateconstant of refolding reached a maximum for all the mutants ataround 2000 s1.

11 Feb 2008: flavin mononucleotide N(3)-H -0.02 0.20 0.03 gxx ) 2.0019anion C(6)-H -0.30 0.48 0.04 gyy ) 2.0041. ... This is correctlypredicted by eqs 2 and 4 above, because the spectral densitydenominators at 20 MHz are no longer small.

6 Jan 2005: 7.4),6 mM MgCl2 20 mM KCl, 1 mM dithioreitol (DTT) (assaybuffer) at 25 8C. ... At times indicated, 2 ml of the reactions werequenched on ice with a final concentration of 20 mMethylene diaminetetraacetic acid (EDTA).

19 Dec 2008: Denaturedsamples at pH 2.9 were left in D2O for 5, 20, 40, or 80 minand then refolded and 15N, 1H correlation spectra of the na-tive state acquired. ... Protein stabilityparameters measured by hydrogen exchange.” Proteins 20, 4–14. Bai, Y, Sosnick, TR

15 Sep 2003: open triangles).(b) Guandinium chloride-induced unfolding of wild-type FKBP12 in the absence (lled circles) and presenceof 20 mM rapamycin (open circles). ... Comparison with other proteins. There are now at least 20 proteins which havebeen reported to

1 Aug 2008: commented on the manuscript. Received: November 20, 2007. Revised: February 22, 2008.

8 Feb 2008: present. as dimer at 1 mM protein. 100 90 20 1 99 96 2 0. ... mM DTT. (B) YibK mutant denaturation profiles for 100. (pink), 50 (dark purple), 20 (light purple), 10.

1 Aug 2008: Im9,20 adenylate kinase,21 and protein A22 under equilibriumconditions. All these studies showed typical two-state-foldingbehavior, and no intermediate states were detected. ... The only reactive Cys residue is the mutation. (20) Tezuka-Kawakami, T.; Gell

19 Apr 2005: Temperature. Protein folding rates display a strong dependence on tem-perature. Folding reactions commonly exhibit activation en-thalpies of 10–20 kJ/mol (e.g., Jackson and Fersht 1991b;Plaxco et ... 56 5.70 0.18 4.20 0.25 32.5 1.4 8.3 0.3

4 Jun 2008: 20 SAXSmeasurements of full-length and truncated Hop. Fig. 5. (a) Solution structure of Cyp40. ... 6 mM MgCl2, 20 mM KCl and 1 mM tris(2-carboxyethyl)phosphine at 25 C.

23 Aug 2004: Bufferswere prepared volumetrically. All stock GdmCl solutionswere prepared in volumetric flasks and stored in aliquots at-20 C until use. ... In comparison, other studies haveshown that single point mutations (V26I or V26L) can resultin an increased

21 Apr 2006: The reduction in thechange in resonance reflects a weaker interactionwith an approximately 20-fold increase in thedissociation constant (33 mM) at 25 8C. ... The major. e spectra were typically acquired at a capillary voltage ofon of 20 mM.

8 Feb 2008: 2.460.01 5.280.01 20.20.110 2.530.01 5.400.1 20.50.420 - - -. Fluorescence was monitored at 350 nm. Parameters are quoted with thto a ... 0.01. 2.50.01. 1.38. 0.01. 20.20.1. 4.40.02. Ybe. ANon. eFL. 0.75. 0.1.