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6 Mar 2001: 0 10 20 300. 25. 50. 75. 100. Time (min). WT 500 µM GTP. ... Cellswere xed with 2% paraformaldehyde/1.5% glutaraldehyde in 0.1 M sodium cacodylatefor 20 min at room temperature.

19 Apr 2006: 2.2. Linear amplification reaction. 2–20 nM of PCR product was used in a linear amplification reaction with 6–400 pM ofpHis17 (B. ... The protein was purified over a Ni-NTA spin column under native conditions (lanes 3 and 5) and separated on a 10–20

3 Dec 2004: we solved the structureof the NH2-terminal domain from the closeAP180 homolog, CALM, at 2 Å resolution(19, 20) (crystals of AP180-N did not diffractwell). ... 20 November 2000; accepted 18 December 2000. Notch Inhibition of RASSignaling Through MAP

1 Jul 2011: 2B). BtubA/B bilden in vitro dimere Filamente,die sich zu einem Komplex aus 20 bis 30 Fila-menten bündeln können.

21 Jun 2005: and size-exclusion chromatography (S300 Sephacryl,Amersham Pharmacia; 20 mM TrisHCl1 mM azide1 mMEDTA, pH 7.5). ... After 30 min at room temperature, samples werecentrifuged at 80,000 rpm for 20 min at 20C in a TLA100 rotor(Beckman).

1 Aug 2000: mp. h2-. SH. 3. 100. 80. 60. 40. 20. 0. α-ap. ... 9 62 ' ' $ ($ )M # 111+ 5 D ( #. ; ;20 ) (# , ;G%0 ( ' 7 # 9 7 >1 7 ) ; / 9#; #$ ' ;G%0 % ' 7 ( ( ;G%0 # 9( ' ( 62 7 7 ( 62 ( ) # --&/ #--8/ # ---#. 3 ( ( ( # 3 ( '

8 Jun 2006: Readings were performed at 20 C using an average of 5 scans with buffer subtracted. ... uals. 6.00 10 20Concentration (µM). 0. 20. 40. 60. 80 (k.

15 Jul 2016: 8 % v/v PEG 550 MME1-36 8 % w/v PEG 20,000/ 0.1 M Sodium acetate 5.5 0.2 M Potassium thiocyanate. ... 20 % v/v Glycerol2-47 1.6 M Sodium/potassium phosphate 6.02-48 1 M Sodium citrate 6.2.

29 May 2017: 60.005 20.000 1 ADP var_2 PA O3A PB O1B 59.979 20.000 1 ADP var_3 O2A PA "O5'" "C5'" -59.942 20.000 1 ADP ... 20.000 1 ADP var_7 "C5'" "C4'" "C3'" "C2'" -150.000 20.000 3.

4 Nov 2022: struction, using local averaging to combine the three-dimensional data from multiplesubsets (17, 20). ... IUCrJ 4,393–399 (2017). 20. A. Miyazawa, Y. Fujiyoshi, M. Stowell, N.

9 Jan 2017: The screens are normally stored at -20 C since they are not used regularly and contain volatile and unstable compounds.

26 Aug 2011: ns. Oral presentation selected from abstracts. 14:15 –16:45 Concurrent session 20.

3 Jul 2013: Eulerangle phi and rot were scanned in a range of 20 around the receptor longaxis estimate. ... J Neurosci 20(2):521–528. 8. Marangi PA, et al. (2001) Acetylcholine receptors are required for agrin-inducedclustering of postsynaptic proteins.

23 Sep 2016: A 1 20 % v/v Acetonitrile (Control of Precipitation). A 2 100 mM MES 5.5 None None.

12 Oct 2006: Radiolabeled 20-bp fragmentsthat lacked KOPS (nKOPS) or that contained a single KOPS (sKOPS). ... 50 l culture. NMR buffer was 20 mM potassium phosphate and 150 mM.

17 Mar 2004: The negative surface potential, which isabout –30 mV for a membrane with 20% phos-phatidylserine [8], also attracts clusters of basicresidues on proteins. ... Dill, K.A., and Bromberg, S. (2003). Molecular Driving Forces.(Garland Science), Chapters

29 Apr 2002: We have previously shown,however, that this parameter is modified by disruption of theexocytotic machinery (19, 20). ... Graham, M. E. & Burgoyne, R. D. (2000) J. Neurosci. 20, 1281–1289.21.

4 Jan 2006: fatty acid-free BSA and 0.01% Triton X-100 with substrate concentrations (20–200 mM LPA) at 25 8C and 37 8C with 14C-labelled oleoyl-CoA (Amersham) at aspecific ... Liposomes made from Folch brain lipid extract at0.2 mg ml21 in 20 mM HEPES pH 7.4, 150

21 Mar 2003: Dynamin GTPase assay and sedimentation assayThese assays have been described previously [20,27] and furtherdetails are given in the Supplementary material. ... Multimerised dynamin was then sedimented by ultracentrifuga-tion at 80,000 g for 20 min.

30 Aug 2001: 1a, b.) The reactions were centrifuged at140,000g in a Beckman TLA100 rotor for 20 min at 20 8C. ... 20. Jones, L. J. F., Carballido-Lopez, R. & Errington, J. Control of cell shape in bacteria: helical, actin-like.

19 Apr 2018: Then, add 20 L of deionized water to the main container of the liquid handler. ... After use, flushing with water and then 20% v/v ethanol solution prevents microbial growth.

23 Nov 2004: Mayer, L.D., Hope, M.J., and Cullis, P.R. (1986). Vesicles ofSigma B1502) were resuspended by sonication at 1 mg/ml in 20 variable sizes produced by a rapid extrusion

3 Dec 2004: we solved the structureof the NH2-terminal domain from the closeAP180 homolog, CALM, at 2 Å resolution(19, 20) (crystals of AP180-N did not diffractwell). ... 20 November 2000; accepted 18 December 2000. Notch Inhibition of RASSignaling Through MAP

3 Sep 2014: Healthcare) in 20 mM Tris, 100 mM NaCl, 1 mM EDTA, 1 mM. ... sucrose, 1 mM EDTA, 20 mM Tris pH 7.6. The cells were stored.

12 Nov 2009: 20% w/v PEG 20 000,40% v/v PEG MME 550. 35 Cordell et al. ... 3 Teo et al. (2006). 20% w/v PEG 4000,40% v/v glycerol.

13 Jun 2003: 20) and Trusca et al. (21), using a MBP-SulAfusion or a protein A-SulA fusion, respectively, found SulA doesinhibit the GTPase activity of FtsZ. ... The reservoir was 7.5%polyethylene glycol (PEG) 20,0007.5% PEG monomethyl ether55080 mM sodium formate0.1

28 Mar 2006: Add to M9 prior to experiment (per litre):15NH4Cl 1g (can dissolve in 10 mL H2O and filter sterilise into media)20% glucose 20 mLvitamins 1 mLtrace elements 10 mLMgSO4 (1M) ... 10 mL of 20% arabinose for BAI cells). Purification:As normal, however

3 May 2002: J. Neurosci. 20,8667–8676. e Farsad, K. et al. (2001) Generation of high curvature membranesmediated by direct endophilin bilayer interactions. ... J. Neurosci. 20, 7986–7993. 35 Schikorski, T. and Stevens, C.F. (2001)Morphological correlates of

19 Dec 2016: unprecedented, with an overall RMSD of 1.6 Å, despite sequence identity of only 20%. ... 7.5. Fractions containing pure arcadin-1 were concentrated to 15–20 mg/ml using a Centriprep concentra-.

23 Nov 2004: These reactionswere performed in KIN buffer {50 mM Mops ( pH 7.2),100 mM NaCl, 20 mM MgCl2, 0.5 mM ATP, and 2 mCiof [g-32P]ATP}. ... we solved the structureof the NH2-terminal domain from the closeAP180 homolog, CALM, at 2 Å resolution(19, 20) (crystals

18 Dec 2008: F) The Saci1373MIM2 (red)/Saci1372 MIT(yellow) interaction isclosely relatedin structurewith the CHMP6 MIM2(green, extended)/VPS4AMIT (gray helices) inter-action (20). ... 19. M. D. Stuchell-Brereton et al., Nature 449, 740 (2007).20. C.

17 Feb 2010: Over the past 20 years,. intensive research has given us a glance into how non-. ... and pellet (P) were analysed on a 10–20% SDS gel,stained with Coomassie.

19 Oct 2022: 20. Coot’s Go To Atom Window (it doesn’t look exactly like this any more). •

23 Jan 2009: 3E)in line with earlier experiments (10, 20, 21), butthe resolution of these images is limited. ... Biol. 156, 246. (2006).20. G. Ebersbach, D. J. Sherratt, K. Gerdes, Mol.

8 Nov 2014: 20, 431 (2014). 7. S. Zhu et al., PLOS Pathog. 9, e1003592 (2013). ... collectively representing 20% of all genes. in a typical eukaryotic genome.

4 May 2004: Only data extending to about 20/ resolution (within the first zero in the contrast transfer function) were used. ... Biochemistry 20, 2181-2191. Conti-Tronconi, B. M., McLane, K. E., Raftery, M.

4 Feb 2015: 20 30. 0 10time (s). 20 30. 0 10time (s). 20 30 0 10time (s). ... 20 30 0 10time (s). 20 30. 0 10time (s). 20 30 0 10time (s).

1 Mar 2024: Abso. rban. ce (a. u.). 50. 00 10 20. Volume (ml)30 0 10 20. ... CdpB2–CdpB1–CdpB1–CdpB2–CdpB2–CdpB1. jMTH1859. B. subtilis YlmCAlphaFold2prediction. Two-fold symmetry. g. i. 20 nm.

14 Sep 2010: There is no ParR or parC present. The scale bar represents 20 nm. ... 20 Macmillan Publishers Limited. All rights reserved10. 7. Simpson, A. E., Skurray, R.

19 Jan 2005: At the MRC Laboratory of Molecular Biology (LMB) about 15–20 groups are more or lessintensely involved in protein crystallisation and/or structure determination. ... Our initial idea for long-term storage of the plateswas to store them at 20 1C to

4 Jan 2024: Nat Struct Mol Biol 20, 641-643. 6. Tourigny, D. S., Fernandez, I.

19 Nov 2004: 40Å. FtsZ-tubulin superposition. outside. a b c d e. 20. 04 N. ... A total of 20 mg pure protein was obtained from 12 l of culture.

5 Jan 2005: dendrites. Tuba [20], anotherBAR protein, has Rho GEF activity and multiple SH3 domains that binddynamin, N-WASP (neural Wiskott–Aldrich syndrome protein) and otheractin-regulating proteins.

18 Jun 2018: 20. Figure 5: Coot’s Go To Atom Window (it doesn’t look exactly like this anymore). •

31 Jan 2020: J. Biol. Chem. 293, 4860–4869 (2018). 20. Yang, S.-X., Pollock, H. ... equili-brated with 20 mM Hepes, 200 mM NaCl at pH 7.5.

30 Apr 1999: Undecoratedtubules. t=20 s t=5 min. on tubules on tubules on tubules. ... Results are the average of three experiments s.e.m. 50 10 15 20 25 300.0.

8 Jun 2006: analytical ultracentrifuge with an An60-Ti rotor, with 39, 78 and 156 µM in 150 mM NaCl, 20. ... at 40 and 150 µM protein in 150 mM NaCl, 20 mM HEPES pH 7.4, 2 mM DTT unless.

16 May 2010: Withsome experience you should be able to get an R-factor of less than 20% in less than30 minutes.

13 Sep 2000: The protein was stored at4 C in 20 mM TRIS, 1 mM EDTA, 1 mM Na-azide. ... P3 yields exclu-sively empty tubes. All reactions were incubated for 20 min at37 C.

18 Dec 2022: S2A). ABC-typeATPases bound to ADP.BeF3 often represent the enzyme–substrate complex (20). ... The peak fractionswere concentrated using a Vivaspin 20 100,000-Da centrifugal concentrator andfrozen.