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Addit04b_AL-SEL-DP Formated
https://www2.mrc-lmb.cam.ac.uk/screens2/pdf/Addit-04b.pdf24 Nov 2020: Z3-12 AZ312 2.8 335.5A 5 5 ANZERGENT 3-14 20 Z3-14 AZ314 0.2 363.6A 6 6 n-Decyl-N,N-dimethylglycine 76 DMG D352 19 ... 0.05 583E 11 59 Polyoxyethylene(10)dodecyl ether 20 C12E10 AP1210? 0.1 627E 12 60 Polyoxyethylene(8)tridecyl ether 20 C13E8 APO138? -
Impress
https://www2.mrc-lmb.cam.ac.uk/coot/ephemera/beyond-coloured-lines.pdf28 Sep 2020: detailed, interactive representation of “high energy”. 20 / 50. Colour-blindness Considerations. ... Slide 18. Slide 19. Slide 20. Slide 21. Slide 22. Slide 23. -
Cryo-EM Model-Buildingwith Modern Coot Paul Emsley@ Ben Gurion…
https://www2.mrc-lmb.cam.ac.uk/personal/pemsley/coot/files/coot-2020-nov-slac.pdf10 Nov 2020: Rank density fit scores,. – Pick top 20 solution, for each of them Rigid body fit and score solutions Pick the highest scoring solution. – ... REFMAC Monomer Library chem_comp_bond. Slide 19. Slide 20. Slide 21. Slide 22. -
MD1-118 Hippocrates additive screen
https://www2.mrc-lmb.cam.ac.uk/groups/JYL/WWWrobots/LMB%20plates/Addit-03.pdf13 Oct 2020: C1 13 9 % Pyridoxine hydrochloride C2 14 9 % Thiamine hydrochloride C3 15 9 % CytidineC4 16 6 % Inosine†C5 17 6 % RibavirinC6 18 6 % ThymidineD1 19 6 % UridineD2 20 1.5 % -
Addit05_NeXtal pH gradient
https://www2.mrc-lmb.cam.ac.uk/screens2/pdf/Addit-05.pdf20 Nov 2020: Sodium Citrate 1 MC6 18 5.6 tri-Sodium Citrate 1 MD1 19 5.8 tri-Sodium Citrate 1 MD2 20 6 Sodium Cacodylate 1 MD3 21 6.2 Sodium Cacodylate -
MD1-116-117 Morpheus III-Morpheus III HT-96
https://www2.mrc-lmb.cam.ac.uk/groups/JYL/WWWrobots/LMB%20plates/LMB22.pdf13 Oct 2020: Catalogue Number (250 mL). 60% Precipitant Mix 1 40% v/v PEG 500 MME; 20 % w/v PEG 20000. ... MD2-100-82 MD2-250-82. 60% Precipitant Mix 3 40% v/v Glycerol; 20% w/v PEG 4000. -
EMBO Practical Course on the Crystallization of Macromolecular…
https://www2.mrc-lmb.cam.ac.uk/groups/cartera/download/cv/2020_01_Carter_CV.pdf31 Jan 2020: Nat Struct Mol Biol. 19: 492-7. 20) Carter AP‡, Cho C, Jin L, Vale RD‡ (2011) Crystal structure of the dynein motor. -
The structure of human thyroglobulin
https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/TG_Nature2020.pdf31 Jan 2020: J. Biol. Chem. 293, 4860–4869 (2018). 20. Yang, S.-X., Pollock, H. ... equili-brated with 20 mM Hepes, 200 mM NaCl at pH 7.5. -
FtsK in motion reveals its mechanism for double-stranded DNA…
https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/FtsK_Jean_2020_PNAS.pdf8 Jun 2020: For thedsDNA, a 20-bp B-form dsDNA model was generated in Coot (32) and fittedmanually inside the map. ... Proc. Natl. Acad. Sci.U.S.A. 114, E697–E706 (2017). 20. A. H. de la Peña, E. -
Identifying proteins bound to native mitotic ESC chromosomes reveals…
https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/Djeghloul%20Nat%20Camms%202020.pdf11 Aug 2020: 40 50. Chromomycin A3. Chromosome 19: Genomic position. 10,000,000 20,000,000 30,000,000 40,000,000 50,000,000 60,000,000. ... μm. 2 )C. entr. omer. e si. ze (. μm2 ). 0. 10. 20. 30. 40. 50. 40. 30. 20 30 40 20 30 40. -
15955888524538 1..29
https://www2.mrc-lmb.cam.ac.uk/groups/hegde/wp-content/uploads/sites/8/2020/09/eLife_2020b.pdf24 Jul 2020: targeting siRNA and analysed by flow cytometry after 20 hr of reporter expression. ... These considerations suggest that a general detector of collisions must be within 20 fold abun-. -
An intramembrane chaperone complex facilitates membrane protein…
https://www2.mrc-lmb.cam.ac.uk/groups/hegde/wp-content/uploads/sites/8/2020/09/Nature_2020.pdf17 Aug 2020: A total of 20,000 GFP-positive (or RFP-positive for SQS and ASGR) cells were collected. ... The transcription reactions were con-ducted with 5–20 ng ml1 PCR product at 37 C. -
16001658465475 1..36
https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/Collier_2020_elife-59560-v1.pdf15 Sep 2020: reagent bis-maleimidoethane (BMOE). Around 20% of cohesin complexes can be crosslinked simul-. ... teins were denatured by adding SDS to a final concentration of 1% and heating at 70˚C for 20 min. -
15919734904046 1..30
https://www2.mrc-lmb.cam.ac.uk/groups/hegde/wp-content/uploads/sites/8/2020/09/eLife_2020.pdf12 Jun 2020: CC1/2 (%) 100.0 (81.7) 100.0 (92.6). Refinement. Rwork/Rfree (%) - 20.3/25.0. ... Micrographs 2776 2484 1206 4228 932. Microscope tilt (degrees) 0 0 30 20 20.
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