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Controlling actin polymerisation in clathrin mediated endocytosis -…
https://www2.mrc-lmb.cam.ac.uk/controlling-actin-polymerisation-in-clathrin-mediated-endocytosis/7 Jun 2018: Work from Harvey McMahon’s group in the LMB’s Neurobiology Division has uncovered how a protein, FCHSD2, controls actin polymerisation during endocytosis. -
MD1-91, MD1-92 and MD1-92-FX Morpheus II 10 mL, HT-96 and FX-96…
https://www2.mrc-lmb.cam.ac.uk/screens2/pdf/LMB20.pdf9 Jul 2018: MD2-100-240. MD2-250-240. 60%Precipitant Mix 7 20% w/v PEG 8000, 40% v/v 1,5-Pentanediol. ... B7 2 mM Divalents II none 30 % v/v Precipitant Mix 71-20 B8 2 mM Divalents II none 31 % v/v Precipitant Mix 81-21 B9 2 mM Divalents II -
Aaron Klug (1926 - 2018) - MRC Laboratory of Molecular Biology
https://www2.mrc-lmb.cam.ac.uk/aaron-klug-1926-2018/21 Nov 2018: Aaron Klug, Director of the LMB from 1986 to 1996, President of The Royal Society from 1995 to 2000 and 1982 Nobel Laureate, died on Tuesday 20. -
The LMB art show goes crafty - MRC Laboratory of Molecular Biology
https://www2.mrc-lmb.cam.ac.uk/the-lmb-art-show-goes-crafty/3 Aug 2018: Over 20 exhibitors participated, with photography being one of the most popular categories. -
ProSHADE: ProSHADE Documentation
https://www2.mrc-lmb.cam.ac.uk/groups/murshudov/content/proshade/doxygen/index.html1 Nov 2018: On line 20, the path to the standard C++ library is required. ... $ proshade -O -f. /2A2Q_T_dom_2_rotated_translated.map -f. /2A2Q_T_dom_2.pdb --clearMap res.pdb --cellBorderSpace 20. -
Richard Henderson made Companion of Honour - MRC Laboratory of…
https://www2.mrc-lmb.cam.ac.uk/richard-henderson-made-companion-of-honour/11 Jun 2018: For the last 20 years, he has been working to improve the methodology of single particle electron cryomicroscopy (cryo-EM), which has recently reached the stage where it is possible to -
Making a cell-based factory for polymer synthesis - MRC Laboratory of …
https://www2.mrc-lmb.cam.ac.uk/making-a-cell-based-factory-for-polymer-synthesis/6 Dec 2018: However, proteins are more complex than most synthetic polymers because they are composed of 20 different compounds (amino acids) strung together in defined order. ... Jason Chin’s group has a long-term interest in creating cell-based factories that -
How cells selectively enhance gene expression in response to stress - …
https://www2.mrc-lmb.cam.ac.uk/how-cells-selectively-enhance-gene-expression-in-response-to-stress/5 Sep 2018: As these 61 different codons encode for 20 different amino acids, most amino acids correspond to more than one codon. -
Two new group leaders at the LMB: Chris Russo and Gerry Crossan - MRC …
https://www2.mrc-lmb.cam.ac.uk/two-new-group-leaders-at-the-lmb-chris-russo-and-gerry-crossan/20 Jul 2018: Published on. 20 July, 2018. The LMB is delighted to announce that Chris Russo and Gerry Crossan have progressed from Independent Investigator Scientist positions to become Group Leaders at the LMB. -
How neuropeptide signalling controls sensitisation in response to…
https://www2.mrc-lmb.cam.ac.uk/how-neuropeptide-signalling-controls-sensitisation-in-response-to-touch-in-c-elegans/24 Aug 2018: In this study, the researchers found that the neuropeptide FLP-20 is released by touch-sensing neurons and leads to sensitisation of chemical-sensing neurons and increased movement speed. ... Yee Lian and Isabel showed that the neuropeptide FLP-20 acts -
Electron cryo-microscopy reveals near-atomic resolution structure of…
https://www2.mrc-lmb.cam.ac.uk/cryo-electron-microscopy-reveals-near-atomic-resolution-structure-of-the-prespliceosome/12 Jul 2018: The human genome contains approximately 20,000 genes, which when transcribed produce precursor messenger RNA (pre-mRNA) consisting of coding sequences (exons) and non-coding sequences (introns). -
Children enjoy an afternoon of discussing science books and hands-on…
https://www2.mrc-lmb.cam.ac.uk/children-enjoy-an-afternoon-of-discussing-science-books-and-hands-on-experiments-with-lmb-postdocs/20 Sep 2018: Published on. 20 September, 2018. -
Instinctive and learned responses to smells are controlled by a…
https://www2.mrc-lmb.cam.ac.uk/instinctive-and-learned-responses-to-smells-are-controlled-by-a-single-brain-circuit-in-flies/20 Sep 2018: Published on. 20 September, 2018. -
LMB researchers are taking part in LifeLab with events around…
https://www2.mrc-lmb.cam.ac.uk/lmb-researchers-are-taking-part-in-lifelab-with-events-around-cambridgeshire-on-friday-28th-and-saturday-29th-september/20 Sep 2018: Published on. 20 September, 2018. LMB researchers are helping to transform parts of Cambridgeshire into centres of discovery with hands-on, family-friendly activities in shopping centres, cafes and public spaces. -
tutorial.dvi
https://www2.mrc-lmb.cam.ac.uk/personal/pemsley/coot/files/newt.pdf18 Jun 2018: 20. Figure 5: Coot’s Go To Atom Window (it doesn’t look exactly like this anymore). • -
Publications
https://www2.mrc-lmb.cam.ac.uk/groups/ms/Publications.html15 Oct 2018: 2010. Mammalian mRNA export requires GANP. Curr. Biol. 20:25-31. A.Giesecke & M.Stewart. ... 1.25 A resolution structure of an RNA 20-mer that binds to the TREX2 complex. -
Manual_CO-311 JBScreen Solubility HTS
https://www2.mrc-lmb.cam.ac.uk/screens2/pdf/SOLUB.pdf19 Mar 2018: In addition, a positive control for precipitation (20% acetonitrile, well# A1) is included in the screen. -
OUTLINE OF RESEARCH
https://www2.mrc-lmb.cam.ac.uk/groups/ms/Research.html15 Jan 2018: Structure, 20:1007-1018. C.Sun, G.Fu, D.Ciziene, M.Stewart & S.M.Musser. (2013). Choreography of Importin? ... 2010). Mammalian mRNA export requires GANP. Curr. Biol. 20:25-31. A.M.Ellisdon, D.Jani, A.Köhler, E.Hurt & M.Stewart. -
mbar-v2
https://www2.mrc-lmb.cam.ac.uk/personal/pemsley/coot/files/presentations/mbar-v2.pdf31 Aug 2018: Under “Weight Matrix”, set the Refinement Weight to 20. 1.2. Automatic model building will only rarely produce a model which is both complete and correct. -
MD1-93 the Morpheus Additive Screen
https://www2.mrc-lmb.cam.ac.uk/screens2/pdf/Addit-01.pdf5 Jul 2018: A1 Water control -A2 PEG 20000 precipitant 20.00 % w/vA3 PEG 500 MME precipitant 40.00 % w/vA4 PEG 8000 precipitant 20.00 % w/vA5 Ethylene glycol cryoprotectant 40.00 % ... trihydrochloride stabilizer 0.20 MH11 1,4-Diaminobutane dihydrochloride -
CCP4 Program Suite: acedrg
https://www2.mrc-lmb.cam.ac.uk/groups/murshudov/content/acedrg/acedrg.html13 Dec 2018: sp3_sp3_112 C27 C26 O3 C25 180.000 10.00 3 UNL sp3_sp3_115 C24 C25 O3 C26 180.000 10.00 3 UNL sp3_sp3_20 C12 C24 -
Coot: Model-building tools for Molecular Graphics
https://www2.mrc-lmb.cam.ac.uk/personal/pemsley/coot/files/presentations/coot-cshl-2018.pdf24 Oct 2018: Slide 19. Slide 20. Slide 21. Slide 22. Slide 23. Slide 24. -
MD1-116-117 Morpheus III-Morpheus III HT-96
https://www2.mrc-lmb.cam.ac.uk/screens2/pdf/LMB22.pdf9 Jul 2018: Catalogue Number (250 mL). 60% Precipitant Mix 1 40% v/v PEG 500 MME; 20 % w/v PEG 20000. ... MD2-100-82 MD2-250-82. 60% Precipitant Mix 3 40% v/v Glycerol; 20% w/v PEG 4000. -
REFMAC5/ProSMART Tutorial
https://www2.mrc-lmb.cam.ac.uk/groups/murshudov/content/tutorials/refmac_tutorial/files/part_4.html23 Jun 2018: Show Me This. Set the "Number of refinement cycles" to 20, and untick "Use hydrogens during refinement". -
MD1-118 Hippocrates additive screen
https://www2.mrc-lmb.cam.ac.uk/screens2/pdf/Addit-03.pdf9 Jul 2018: C1 13 9 % Pyridoxine hydrochloride C2 14 9 % Thiamine hydrochloride C3 15 9 % CytidineC4 16 6 % Inosine†C5 17 6 % RibavirinC6 18 6 % ThymidineD1 19 6 % UridineD2 20 1.5 % -
ProSHADE: src/bin/bin.cpp Source File
https://www2.mrc-lmb.cam.ac.uk/groups/murshudov/content/proshade/doxygen/d2/db3/bin_8cpp_source.html1 Nov 2018: 961 setUp->peakDistanceForReal = 0.20;. -
MD1-46, MD1-47 and MD1-47-FX Morpheus 10 mL, HT-96 and FX-96…
https://www2.mrc-lmb.cam.ac.uk/screens2/pdf/LMB17.pdf9 Jul 2018: 60% Precipitant Mix 1 P500MME_P20K 40% v/v PEG 500 MME; 20 % w/v PEG 20000. ... MD2-100-82 MD2-250-82. 60% Precipitant Mix 3 GOL_P4K 40% v/v Glycerol; 20% w/v PEG 4000. -
Journal of Visualized Experiments www.jove.com Copyright © 2018…
https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/JOVE2018.pdf19 Apr 2018: Then, add 20 L of deionized water to the main container of the liquid handler. ... After use, flushing with water and then 20% v/v ethanol solution prevents microbial growth. -
ProSHADE: src/proshade/ProSHADE_lapack.cpp Source File
https://www2.mrc-lmb.cam.ac.uk/groups/murshudov/content/proshade/doxygen/d7/dee/_pro_s_h_a_d_e__lapack_8cpp_source.html1 Nov 2018: 1. 20 //============================================ Lapack-E. -
Cryo-EM reconstruction of AlfA from Bacillus subtilis reveals the…
https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/AlfA2018.pdf20 Mar 2018: The locus codesfor the ParMRC system that, besides ParM, contains the DNA-binding protein ParR, and a centromeric sequence parC (19, 20).Principles governing plasmid segregation by the ParMRC systemare well ... J Mol Biol269:505–513. 20. Popp D, et al. -
Coot: Model-building tools for Molecular Graphics
https://www2.mrc-lmb.cam.ac.uk/personal/pemsley/coot/files/presentations/bgu/coot-2018-bgu.pdf22 Feb 2018: Slide 20. Slide 21. Slide 22. Slide 23. Slide 24. Slide 25. -
Coot Tools for Cryo-EM Paul EmsleyMRC Laboratory of Molecular ...
https://www2.mrc-lmb.cam.ac.uk/personal/pemsley/coot/files/presentations/coot-cryoEM-2018-Oct-TF.pdf8 Oct 2018: Slide 16. Slide 17. Slide 18. Slide 19. Slide 20. Slide 21. -
Interactive Tools forCryo-EM Map Fitting Paul EmsleyMRC Laboratory of …
https://www2.mrc-lmb.cam.ac.uk/personal/pemsley/coot/files/presentations/coot-cryoEM-2018-June-PSI.pdf19 Jun 2018: Slide 19. Slide 20. Slide 21. Slide 22. Slide 23. Slide 24. -
Manipulating Ligands Using Coot with a bit of carbohydrates ...
https://www2.mrc-lmb.cam.ac.uk/personal/pemsley/coot/files/presentations/bgu/coot-feb-2018-ligands-carbohydrate-bgu.pdf22 Feb 2018: 60.005 20.000 1 ADP var_2 PA O3A PB O1B 59.979 20.000 1 ADP var_3 O2A PA "O5'" "C5'" -59.942 20.000 1 ADP ... 20.000 1 ADP var_7 "C5'" "C4'" "C3'" "C2'" -150.000 20.000 3. -
Coot Tools for Cryo-EM Paul EmsleyMRC Laboratory of Molecular ...
https://www2.mrc-lmb.cam.ac.uk/personal/pemsley/coot/files/presentations/coot-cryoEM-2018-Aug-Stockholm.pdf15 Aug 2018: Slide 17. Slide 18. Slide 19. Slide 20. Slide 21. Slide 22. -
mbar-v2
https://www2.mrc-lmb.cam.ac.uk/personal/pemsley/coot/web/tutorial/model-building-and-refinement-v2.pdf31 Aug 2018: Under “Weight Matrix”, set the Refinement Weight to 20. 1.2. Automatic model building will only rarely produce a model which is both complete and correct. -
Prokaryotic cytoskeletons: protein filaments organizing small cells
https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/nrmicro.2017.153.pdf18 Jan 2018: Nature Reviews Microbiology, (2018). doi:10.1038/nrmicro.2017.153 -
ProSHADE: src/proshade/ProSHADE_internal.h Source File
https://www2.mrc-lmb.cam.ac.uk/groups/murshudov/content/proshade/doxygen/db/db2/_pro_s_h_a_d_e__internal_8h_source.html1 Nov 2018: 20 #include "ProSHADE_version.h". -
ProSHADE: src/proshade/ProSHADE_clipper.cpp Source File
https://www2.mrc-lmb.cam.ac.uk/groups/murshudov/content/proshade/doxygen/d9/d66/_pro_s_h_a_d_e__clipper_8cpp_source.html1 Nov 2018: 240 double ecsHlp = std::floor ( std::max ( 20.0, ( this->_maxMapRange / 4.0 ) ) );. 241. ... 764 while ( std::floor ( this->_maxMapRange / this->_shellSpacing ) < 20 ). 765 {. 766 this->_shellSpacing /= 2.0;. -
ProSHADE: src/proshade/ProSHADE_internal.cpp Source File
https://www2.mrc-lmb.cam.ac.uk/groups/murshudov/content/proshade/doxygen/d9/dc4/_pro_s_h_a_d_e__internal_8cpp_source.html1 Nov 2018: 1. 20 //============================================ Clipper. -
ProSHADE: src/proshade/ProSHADE_SO3transform.cpp Source File
https://www2.mrc-lmb.cam.ac.uk/groups/murshudov/content/proshade/doxygen/d7/db2/_pro_s_h_a_d_e___s_o3transform_8cpp_source.html1 Nov 2018: ProSHADE 0.6.5 (NOV 2018). Protein Shape Descriptors and Symmetry Detection. ProSHADE_SO3transform.cpp. Go to the documentation of this file.. 1. 21 //============================================ FFTW3 SOFT. 22 #ifdef __cplusplus. 23 extern "C" {. -
The ER membrane protein complex is a transmembrane domain insertase
https://www2.mrc-lmb.cam.ac.uk/groups/hegde/download/Science_2017c.pdf27 Jan 2018: TA proteins were translated for 20 minutes at 32 C unless otherwise indicated. ... The insertion reaction proceeded at 32 C for 20 minutes unless otherwise stated. -
ProSHADE: src/proshade/ProSHADE_spharmonickit.cpp Source File
https://www2.mrc-lmb.cam.ac.uk/groups/murshudov/content/proshade/doxygen/d8/dd5/_pro_s_h_a_d_e__spharmonickit_8cpp_source.html1 Nov 2018: 1. 20 //============================================ FFTW3 SOFT. -
15211199233993 1..45
https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/MreBHussain2018.pdf15 Mar 2018: B) Confined cells induced at 3 mM. xylose in 20 mM Mg2+ progressively swell upon escaping confinement into free culture. ... Bottom) BRB785 cells in 20 mM Mg2+ were depleted of Pbp2a by growth in 0 mM IPTG. -
ProSHADE: src/proshade/ProSHADE_descriptors.cpp Source File
https://www2.mrc-lmb.cam.ac.uk/groups/murshudov/content/proshade/doxygen/df/d91/_pro_s_h_a_d_e__descriptors_8cpp_source.html1 Nov 2018: 820 //======================== Integrate over all radii using 20-point Gauss-Legendre. -
Morpheus stock solutions poster portrait A2
https://www2.mrc-lmb.cam.ac.uk/groups/JYL/WWWrobots/PDF/PresentationsOther/Morpheus_stock_solutions_poster.pdf1 Jun 2018: Buffer system 3 MORPHEUS I & III MD2-100-102 Two stocks: 1 M bicine (acid, pKa 8.4) and 1 M Trizma-base (pKa 8.20).
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