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The final 20 minutes before leaving (and stretching my ...
https://www2.mrc-lmb.cam.ac.uk/coot/pictures/2010-may-Montevideo/medium/PICT1280-page.html28 Jul 2017: The final 20 minutes before leaving (and stretching my camera to its (hand-held) limit). -
Success for the LMB at the MRC CEO Awards - MRC Laboratory of…
https://www2.mrc-lmb.cam.ac.uk/success-lmb-mrc-ceo-awards/21 Dec 2017: a new ‘challenge project’ for 20 year 11 pupils in early 2018 based around DNA, taste and nematode worm behaviour. -
Researchers "drug the undruggable" - MRC Laboratory of…
https://www2.mrc-lmb.cam.ac.uk/researchers-drug-undruggable/20 Oct 2017: Published on. 20 October, 2017. A new approach to targeting key cancer-linked proteins, thought to be ‘undruggable’, has been discovered through an alliance between industry and academia created by Cancer -
LMB staff are cycle enthusiasts - MRC Laboratory of Molecular Biology
https://www2.mrc-lmb.cam.ac.uk/lmb-staff-cycle-enthusiasts/20 Apr 2017: Published on. 20 April, 2017. The LMB won the National Cycling Challenge for the 200-499 employees category in 2016, with the team cycling 9,969 miles in the month of -
Sydney Brenner: A Revolutionary Biologist - MRC Laboratory of…
https://www2.mrc-lmb.cam.ac.uk/sydney-brenner-revolutionary-biologist/30 Nov 2017: Published on. 30 November, 2017. Sydney Brenner, one of the 20. ... th. Century’s greatest biologists and joint winner of the 2002 Nobel Prize for Physiology and Medicine, talks to biologist and historian Matthew Cobb about his route into science, his -
Mechanism for cleaning up leftover proteins - MRC Laboratory of…
https://www2.mrc-lmb.cam.ac.uk/mechanism-cleaning-leftover-proteins/4 Aug 2017: A 20-year old observation that UBE2O is highly expressed in red blood cell precursors provided a clue. -
Sjors Scheres elected to EMBO membership - MRC Laboratory of…
https://www2.mrc-lmb.cam.ac.uk/sjors-scheres-elected-embo-membership/16 Jun 2017: th. -20. th. October 2017. Primary Sidebar. Search. Search this website. -
Robotic crystallisation: References, presentations, other links
https://www2.mrc-lmb.cam.ac.uk/groups/JYL/WWWrobots/presentations.html21 Sep 2017: Formulation of the MORPHEUS protein crystallization screens (LMB 17, 20 and 22). -
Max Perutz Lecture to be given by Caroline Dean - MRC Laboratory of…
https://www2.mrc-lmb.cam.ac.uk/max-perutz-lecture-given-caroline-dean/20 Apr 2017: 20 April, 2017. FEBS/EMBO. Caroline Dean will give the 2016 Max Perutz Lecture on Thursday 27. -
LMB cycles to first place in September Challenge - MRC Laboratory of…
https://www2.mrc-lmb.cam.ac.uk/lmb-cycles-first-place-september-challenge/1 Nov 2017: This year 20,300 people from 3,000 workplaces across the UK took part in the Challenge. -
Uncovering the molecular basis of triage during protein synthesis -…
https://www2.mrc-lmb.cam.ac.uk/uncovering-molecular-basis-triage-protein-synthesis/20 Jan 2017: 20 January, 2017. Every minute, cells make millions of new proteins which must be transported to the correct location, folded, modified and assembled with other proteins in order to function properly. ... In this way, the length of time substrates have -
The UK’s dementia research landscape: LMB is a piece of the jigsaw -…
https://www2.mrc-lmb.cam.ac.uk/uks-dementia-research-landscape-lmb-piece-jigsaw/20 Sep 2017: Published on. 20 September, 2017. -
Brad Amos to talk at Cambridge Science Festival: Monday 20th March,…
https://www2.mrc-lmb.cam.ac.uk/brad-amos-talk-cambridge-science-festival-monday-20th-march-6-00pm/27 Feb 2017: 20 March: 6:00pm – 7:00pm, UTC Cambridge, Robinson Way, CB2 0SZ. -
Model-Building with Coot Adding N-linked Glycosylation& Demo Paul …
https://www2.mrc-lmb.cam.ac.uk/personal/pemsley/coot/files/coot-may-2017-aca-new-orleans.pdf29 May 2017: 60.005 20.000 1 ADP var_2 PA O3A PB O1B 59.979 20.000 1 ADP var_3 O2A PA "O5'" "C5'" -59.942 20.000 1 ADP ... 20.000 1 ADP var_7 "C5'" "C4'" "C3'" "C2'" -150.000 20.000 3. -
MRC-LMB robotic crystallisation: Technology Overview
https://www2.mrc-lmb.cam.ac.uk/groups/JYL/WWWrobots/tech-overview.html9 Mar 2017: To facilitate high-throughput, a collaboration was established with Hamilton Robotics (2011) to develop a fully automated system that set-up 1-20 crystallisation plates. -
Comparing AceDRG vs CSD LIGANDS Meeting Sept 2017 Rob ...
https://www2.mrc-lmb.cam.ac.uk/personal/pemsley/coot/files/presentations/2017_Sept_Ligands.pdf29 Sep 2017: 19. AOM : 1lho. AOM (CSD). 20. AOM : 1lho. AOM (CSD). -
Protocol for Hamilton LABSTAR
https://www2.mrc-lmb.cam.ac.uk/groups/JYL/WWWrobots/PDF/Manuals/Protocol_for_LabStar.pdf11 Dec 2017: PROTOCOL System 2: setting up crystallization droplets (100 nL protein 100 nL condition) from a single sample in 20 LMB plates 1. ... 15. Clean the SBS lids with a 20% ethanol solution before stacking them on the left-hand side of the liquid handler for -
1 Coot Updates Paul EmsleySept 2017 2 N-linked Carbohydrates● ...
https://www2.mrc-lmb.cam.ac.uk/personal/pemsley/coot/files/presentations/Coot-CCP4-Ligands-Sept-2017-v2.pdf29 Sep 2017: 19. Puzzle 5-2: Annealed. 20. Puzzle 5-2: Comparison. 21. Puzzles 5-2: Coot model is better? ... Slide 19. Slide 20. Slide 21. -
Pymol guide
https://www2.mrc-lmb.cam.ac.uk/groups/nagai/download/pymolguide.pdf19 Dec 2017: 1026.595825195, 277.550354004, 267.541595459, 277.006958008, 827.363769531, 1226.079711914, -20.000000000 ). Conclusion Hopefully you are now confident to explore the spliceosome using our PyMOL sessions. -
Manipulating Ligands Using Coot with a bit of carbohydrates ...
https://www2.mrc-lmb.cam.ac.uk/personal/pemsley/coot/files/coot-may-2017-ligands-madrid-mcs2017-csic.pdf10 May 2017: 60.005 20.000 1 ADP var_2 PA O3A PB O1B 59.979 20.000 1 ADP var_3 O2A PA "O5'" "C5'" -59.942 20.000 1 ADP ... 20.000 1 ADP var_7 "C5'" "C4'" "C3'" "C2'" -150.000 20.000 3. -
Protocol for dragonfly
https://www2.mrc-lmb.cam.ac.uk/groups/JYL/WWWrobots/PDF/Manuals/protocols_for_dragonfly.pdf13 Dec 2017: The advanced setting for ‘max shot vol’ should be lowered from 6,000 to 3,000 when using solutions containing [isopropanol] > 10% v/v and [MPD] > 20% v/v. ... secMAXI 24 200 1.8 2 min 25 secMAXI 48 200 3 4 min 20 sec. -
Protocol for Tecan EVO
https://www2.mrc-lmb.cam.ac.uk/groups/JYL/WWWrobots/PDF/Manuals/Protocol-for-Tecan-EVO.pdf11 Dec 2017: 3. Then, add 20 L of deionized water to the main container of the liquid handler. ... Disconnect the coupling inserts from the small container (20% ethanol rinsing solution) and connect the inserts to the main container. -
MergedFile
https://www2.mrc-lmb.cam.ac.uk/groups/nu/pdf/iucrj17.pdf24 May 2017: For three-. dimensional classification, an initial 20 Å resolution model. was generated by Fourier–Bessel synthesis from structure-. -
Structure of the hexagonal surface layer on Caulobacter crescentus…
https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/s-layer-nmicrobiol201759.pdf6 Apr 2017: The central pore is 20 Å wide, and the lattice is70 Å thick. ... J. Bacteriol. 192, 5855–5865 (2010). 20. Smit, J., Engelhardt, H., Volker, S., Smith, S. -
Protocol for dragonfly copy
https://www2.mrc-lmb.cam.ac.uk/groups/JYL/WWWrobots/PDF/Manuals/Protocol_mosquito_additive.pdf13 Dec 2017: Rinse the container of condition with deionized water and 20 % ethanol for reuse. ... 5. Seal the cell culture plate containing the screen with an aluminum sheet and place it back in the -20 C incubator. -
Optimization of crystallization conditions with additive screening…
https://www2.mrc-lmb.cam.ac.uk/groups/JYL/WWWrobots/PDF/PresentationsOther/additive-screening.pdf9 Jan 2017: The screens are normally stored at -20 C since they are not used regularly and contain volatile and unstable compounds. -
REPORT◥ PROTEOSTASIS UBE2O is a quality control factor fororphans ...
https://www2.mrc-lmb.cam.ac.uk/groups/hegde/download/Science_2017b.pdf4 Aug 2017: S1 to S11References (30–41). 20 February 2017; accepted 8 June 201710.1126/science.aan0178. ... EDTA (Roche) for 20 min and lysed with 26G needle attached to a syringe. -
REPORT◥ PROTEIN HOMEOSTASIS Mechanistic basis for a moleculartriage…
https://www2.mrc-lmb.cam.ac.uk/groups/hegde/download/Science_2017.pdf19 Jan 2017: TRC40x SGTA -x TRC40 -. time (sec): 0 10 20 30 45 60 300. ... Cell Sci. 127,. 4728–4739 (2014).20. P. Leznicki, S. High, Proc. Natl. -
IL-17 is a neuromodulator of Caenorhabditis elegans sensory responses
https://www2.mrc-lmb.cam.ac.uk/groups/hegde/download/Nature_2017.pdf13 Jan 2017: 1f)20. The ilc-17.1, ilcr-1, and ilcr-2 mutants responded to 21% O2 immediately after being transferred to the. ... 20. 40. 60. 80. 100. 0Sp. eed. (μm. s–1. ) t = 2 h. -
Four-stranded mini microtubules formed by Prosthecobacter BtubAB show …
https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/PNAS-2017-Deng-E5950-8.pdf29 Dec 2017: TPR11 helices. 12. 34. 20 nm50 nm. BtubAB micrograph. kinesin light chainPDB 3ZFW. ... Proto-filaments contain alternating A and B subunits, as was suggested(7, 20). -
A Polymerization-Associated Structural Switch in FtsZ That Enables…
https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/mBio-2017-Wagstaff-.pdf3 May 2017: It is difficult to explain how the kineticpolarity needed for treadmilling can be coupled to the structural polarity of single-stranded filaments, such as those of FtsZ (20). ... B) GTPase activity of FtsZ proteinsat 10 and 20 M in the presence of GTP or -
doi:10.1016/j.pbiomolbio.2004.07.009
https://www2.mrc-lmb.cam.ac.uk/groups/JYL/WWWrobots/PDF/Publications/Robotic_nanolitre_protein_crystallisation.pdf22 Sep 2017: At the MRC Laboratory of Molecular Biology (LMB) about 15–20 groups are more or lessintensely involved in protein crystallisation and/or structure determination. ... Our initial idea for long-term storage of the plateswas to store them at 20 1C to
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