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Reproducible Imaging and Dissection of Plasmid DNA Under Liquid with…
www.damtp.cam.ac.uk/user/gold/pdfs/teaching/ufk_papers/AFM/hansma.pdf23 Sep 2010: Plasmid strands are 3 to 6 nm wide in (A) to (D), and 15 to 20 nm in (F). ... DNA is more easily dissect- ed in a 80:20 propanol-water mixture than in propanol alone. -
Biophysical Journal Volume 72 March 1997 1335-1346 Stretching DNA ...
www.damtp.cam.ac.uk/user/gold/pdfs/teaching/ufk_papers/DNA/wang.pdf13 Nov 2010: Clamp Off Clamp On. 0 20 40 60 80 100 120 0 20 40 60 80 100 120. ... 50. 40. 30. --. z. aL)L.0U. 20. 10. 0. Force-extension relations. -
Mesoscale conformational changes in theDNA-repair complex…
www.damtp.cam.ac.uk/user/gold/pdfs/teaching/ufk_papers/AFM/moreno.pdf22 Oct 2010: a, Definition of angle a ( (a1 þ a2)/2) and distance d.Movies composed of 6–20 frames were acquired for 17 different hR/Mmolecules. ... A 20 ml portion of the dilutedsolution (400 ng of protein complex in 150 mM KCl, 25 mM Tris-HCl pH 8.0,10% glycerol) -
Fawcett_Cam-Final
www.damtp.cam.ac.uk/user/astro/conference_web/talks/Fawcett_Mason2010.pdf22 Nov 2010: 1960). 1. Edlén, B. (1942). Z. Astrophys. 20, 30. 2. Edlén, B. -
LETTERS Direct force measurements on DNA in asolid-state nanopore ...
www.damtp.cam.ac.uk/user/gold/pdfs/teaching/ufk_papers/nanopores/nphys344.pdf21 Nov 2010: ts. ΔZ. ΔZ = Fel/ktrap. DNA ejected fromnanopore. 10 20 300 40Time (ms). ... 0.01 0.1 1. 75%. 10. 0. 20. 30. Voltage (mV). Forc. -
jas3293 1261..1274
www.damtp.cam.ac.uk/user/mem/wood-mcintyre-jets-jas10.pdf27 May 2010: Q(x, y; z)d =2c 11. r0(z). ›. ›zr. 0(z). f 20. ... N(z)2›c. ›z! 1f 20. N(z0)2. ›c. ›zd(z z. 0) 1 by, (4.1). -
DOI: 10.1126/science.276.5315.1109 , 1109 (1997); 276Science et…
www.damtp.cam.ac.uk/user/gold/pdfs/teaching/ufk_papers/proteins/rief.pdf8 Oct 2010: Protein was storedfrozen in aliquots in 20 mM sodium phosphate (pH 7)and 5 mM dithiothreitol (DTT). ... J. 72, 1541 (1997).20. G. I. Bell, Science 200, 618 (1978).21. -
No Job Name
www.damtp.cam.ac.uk/user/gold/pdfs/teaching/ufk_papers/nanopores/smeets.pdf2 Nov 2010: Theresulting free-standing silicon nitride membrane was coveredwith a thin layer of sputtered silicon oxide (20 nm) onboth sides. ... J.; Aluru, N. R.; Timp, G.Proc. Natl. Acad. Sci. U.S.A. 2005, 102, 10445.(20) Siwy, Z.; Kosinska, I. -
Solar Plasma Spectroscopy: Achievements and Future Challenges 13-15…
www.damtp.cam.ac.uk/user/astro/conference_web/conprogram.pdf10 Sep 2010: 17:00 - 17:20. Conference Reception 17:45 - 19:00. Tuesday - 14th September - 2010. ... Stephen Bradshaw Non-equilibrium process in the Solar Corona 16:20 - 16:40. -
Proc. Nati. Acad. Sci. USAVol. 89, pp. 8721-8725, September ...
www.damtp.cam.ac.uk/user/gold/pdfs/teaching/ufk_papers/proteins/foldingfunnel.pdf4 Oct 2010: the. same 20-letter set. Although no structural or reconfigura-tional criteria were used to determine S2, it does have adistinct global minimum. ... Thereconfigurational distances between the low energy S2 conforma-tions and its lowest energy structure -
Proc. Natl. Acad. Sci. USAVol. 93, pp. 13770–13773, November ...
www.damtp.cam.ac.uk/user/gold/pdfs/teaching/ufk_papers/nanopores/kasianowicz.pdf21 Nov 2010: O.,Hanover, J. A.&Leipins,A. (1995) J.Membr. Biol. 146, 239–251.20. Bustamante,J. O.,Oberleithner,H.,Hanover,J. A.&Liepins,A. ... to the cischambercontainingeither0.13mgymlpoly[U] () (mean length,1100nt) or 0.27 mgyml poly[A] (M) (mean length, 20,000 nt -
npgrj_nprot_2007-309 2191..2197
www.damtp.cam.ac.uk/user/gold/pdfs/teaching/ufk_papers/AFM/muller-engel.pdf22 Oct 2010: For these measurements, AFMstylus and support should be separated by at least 20 mm. ... J. Microsc. 169,75–84 (1993). 20. Engel, A., Schoenenberger, C.A. & Müller, D.J. -
VOLUME 88, NUMBER 12 P H Y S I ...
www.damtp.cam.ac.uk/user/gold/pdfs/teaching/ufk_papers/entropic_trapping/craighead.pdf16 Nov 2010: escape from entropic traps [20], wherein ther-mal fluctuations drive a small portion of the chain over the. ... T2 phageDNA molecules, with a fully stretched length of nearly51 mm, were suspended in Tris-borate EDTA buffer andstained with YOYO-1 dye -
LECTURE NOTES FOR IIB PARTIAL DIFFERENTIALEQUATIONSM. S. JOSHI AND ...
www.damtp.cam.ac.uk/user/dmas2/public_ps/pdewj.pdf27 Oct 2010: By change of variablesLu = z@v@r ir @v@ zi@v@z( Check! )Set V (r;z) = iR 20 N(r;;z)d, a C0 function on 0 < r < R, jzj < ... 20 M. S. JOSHI AND A. J. WASSERMANNWe start by showing that the Cauchy problem always has a solutionfor 1st order linear PDEs with -
Engel (review)
www.damtp.cam.ac.uk/user/gold/pdfs/teaching/ufk_papers/AFM/engel-muller.pdf23 Sep 2010: To study theirdynamics, small cantilevers now allow real time measurements atscanning rates of 20 Hz or faster10. ... Engel, A. J. Mol. Biol. 285,. 1903–1909 (1999).20. Scheuring, S. -
..............................................................…
www.damtp.cam.ac.uk/user/gold/pdfs/teaching/ufk_papers/magnetic_tweezers/koster.pdf16 Nov 2010: Itthus seems unrealistic that any religation events would be observedwith a separation of 20 Å. ... 21 BSA. TopIBconcentrations were between 0.5 and 20 nM. The rotation rate measurements wereperformed in identical buffer except that 2 mM MgCl2 was used. -
J412_12c 166..169
www.damtp.cam.ac.uk/user/gold/pdfs/teaching/ufk_papers/nanopores/golovchenko2001.pdf2 Nov 2010: Jupiter's small satellites. Icarus 145, 445453 (2000). 20. Farinella, P. et al. ... 100. 60. 20. 0 200 400. 22 C. 10 C. –10 C10 C. -
LETTERS Antitumour drugs impede DNA uncoiling bytopoisomerase IDaniel …
www.damtp.cam.ac.uk/user/gold/pdfs/teaching/ufk_papers/magnetic_tweezers/Koster2.pdf16 Nov 2010: 0. 20. Cou. nts. Cou. ntsCou. nts. Religation time (s). Religation time (s). ... 15. 10. 5. 0. 4. 2. 0–10 0 10. 620. 20 30. -
Proc. Natl. Acad. Sci. USAVol. 95, pp. 14152–14157, November ...
www.damtp.cam.ac.uk/user/gold/pdfs/teaching/ufk_papers/magnetic_tweezers/allemand.pdf17 Nov 2010: This corresponds to s ,20.015 and forces. 0.3 pN or s. ... By stretching the molecule andpreventing its writhing, we observe denaturation already ats ' 20.015. -
Graphene as a subnanometre trans-electrode membrane
www.damtp.cam.ac.uk/user/gold/pdfs/teaching/ufk_papers/nanopores/golovchenko2010.pdf13 Nov 2010: The best fit to the measured pore conductance datain Fig. 3 yields LIT 5 0.6 (10.9, 20.6) nm, with the uncertainty deter-mined from a least-squares error ... 450 uC in a stream of gas containing 4% H2 in He for 20 min to drive off anyremaining -
On wave-action and its relatives
www.damtp.cam.ac.uk/user/mem/andrews-mcintyre-waveac-jfm78.pdf22 Jul 2010: V x ( i i L - p ) = O (5.20). -
ARTICLES nature materials | VOL 2 | SEPTEMBER 2003 ...
www.damtp.cam.ac.uk/user/gold/pdfs/teaching/ufk_papers/nanopores/golovchenko2003.pdf2 Nov 2010: Isolated regions with only one event in a 20-µs 2-pA bin are not displayed inthis plot.The colour scale is the same as in Fig. ... Experiments with 50-kb DNA and a15–20-nm pore (data not shown) also show three-level blockades. -
Fast dynamics of supercoiled DNA revealedby single-molecule…
www.damtp.cam.ac.uk/user/gold/pdfs/teaching/ufk_papers/magnetic_tweezers/crut.pdf17 Nov 2010: 20. Forceswitch. (2)(2). Nick. (1)(1). Fig. 4. Scenarios of experiments involving supercoiled DNA. ... Materials and MethodsExperimental Setup. All experiments were performed with 20.7 kblong DNA molecules containing a unique site for the nickingenzyme -
b902072c 939..947
www.damtp.cam.ac.uk/user/gold/pdfs/teaching/ufk_papers/nanopores/keyser.pdf4 Oct 2010: technique to measure electrophoretic mobility in bulk. solution.20 This is probably one of the best techniques to. ... E. Godfrey, Proc. Natl. Acad. Sci. U. S. A., 1989, 86, 4479.20 T. -
J397_14c 141..144
www.damtp.cam.ac.uk/user/gold/pdfs/teaching/ufk_papers/entropic_trapping/asher.pdf16 Nov 2010: The switching event inducesatomic displacements of 20 60 AÊ. It has been recognized for a long time that the BZ transitioncould be used to drive a DNA-based mechanical device15, but ... is a 20-nucleotide region of proto-Z DNA in the B-DNA conformation, -
Lect12.dvi
www.damtp.cam.ac.uk/user/na/PartIB/Lect12.pdf18 Feb 2010: n = 10 104 sec., n = 20 17 min, n = 30 4 105 years. -
Back pages NEW1
www.damtp.cam.ac.uk/user/gold/pdfs/teaching/ufk_papers/optical_tweezers/bustamante2003.pdf30 Oct 2010: This. dsDNA. ssDNA. a. Pipette. 0. 20. 40. 60. 80. 0 0.5 1 1.5 2. ... The same ‘backsliding’phenomenon may be responsible for the steep drop in transcriptionalvelocity seen as opposing force is increased above 20 pN (ref. -
An exact theory of nonlinear waves on a Lagrangian-mean flow
www.damtp.cam.ac.uk/user/mem/andrews-mcintyre-glm-jfm78.pdf22 Jul 2010: D S / D t Q = 0, (2.20). D q j L Q" = 0, (2.21). -
Single colloid electrophoresis
www.damtp.cam.ac.uk/user/gold/pdfs/teaching/ufk_papers/electrokinetics/colloid-electrophoresis2.pdf4 Oct 2010: Article history:Received 20 March 2009Accepted 11 May 2009Available online 18 May 2009. ... Daunweg, V. Lobaskin, K. Seethalakshmy-Hariharan, C. Holm, J. Phys. Condens. Matter 20 (40) (2008) 404214.[26] A.G. -
Optical tweezers for force measurements on DNA in nanoporesU. ...
www.damtp.cam.ac.uk/user/gold/pdfs/teaching/ufk_papers/optical_tweezers/keyser2006.pdf28 Oct 2010: Our thin membranes 20–60 nm thickness con-taining the nanopores usually have a diameter of 5 µm. ... By fitting the peak with a2D Gaussian we can localize the nanopores with an accuracyof 20 nm with respect to the edge of the membrane. -
Proc. Natl. Acad. Sci. USAVol. 94, pp. 4853–4860, May ...
www.damtp.cam.ac.uk/user/gold/pdfs/teaching/ufk_papers/optical_tweezers/ashkin.pdf1 Nov 2010: The equilibrium temperature finally achieved is a bal-ance of the optical cooling rate and the quantum heating rate.Letokhov and Minogin (20, 21) were the first to estimate theequilibrium temperature ... subsequent choice oftraps.Themolassesexperiment (33 -
Laser-Induced Heating in Optical Traps
www.damtp.cam.ac.uk/user/gold/pdfs/teaching/ufk_papers/optical_tweezers/peterman.pdf16 Nov 2010: Sðf Þ5 S0 3 f20. f 20 1 f 2; S0 [. 4g 3 kB 3 Tk2. ; f0 [k. 2p 3 g; (1). ... 1310 Peterman et al. Biophysical Journal 84(2) 1308–1316. 1S0 3 f 20. -
Visualizing Protein-Nucleic Acid Interactions on a Large Scale with…
www.damtp.cam.ac.uk/user/gold/pdfs/teaching/ufk_papers/AFM/rivetti.pdf13 Nov 2010: Annu. Rev. Biophys. Biomol. Struct. 1996. 25:395-429 Copyright 0 I996 by Annual Reviews Inc. All rights reserved. VISUALIZING. INTERACTIONS ON A LARGE SCALE WITH THE SCANNING FORCE MICROSCOPE. PROTEIN-NUCLEIC ACID. Carlos Bustamante" and Claudio -
Optical tweezers with 2.5 kHz bandwidth video detectionfor…
www.damtp.cam.ac.uk/user/gold/pdfs/teaching/ufk_papers/electrokinetics/colloid-electrophoresis1.pdf4 Oct 2010: Received 20 November 2007; accepted 29 January 2008; published online 28 February 2008. ... Goldman, and P. R. Selvin, Science 300, 2061 2003.20 J. Gelles, B. -
Lect16.dvi
www.damtp.cam.ac.uk/user/na/PartIB/Lect16.pdf24 Feb 2010: Example. A =. . . . . . . 2 4 70 3 10 0 20 0 10 0 2. -
Magnetic Tweezers: Micromanipulation and Force Measurement at the…
www.damtp.cam.ac.uk/user/gold/pdfs/teaching/ufk_papers/magnetic_tweezers/croquette.pdf10 Nov 2010: Those two alloys were chosen for theirlow remanent magnetization (20 gauss for mumetal). ... The tweezers develop a force along z varying from 50fN to 20 pN. -
Problems and prospects in the theory of gel electrophoresis of DNA
www.damtp.cam.ac.uk/user/gold/pdfs/teaching/ufk_papers/electrokinetics/zimm.pdf18 Nov 2010: Quarterly Reviews of Biophysics 25, 2 (1992), pp. 171-204 171Printed in Great Britain. Problems and prospects in the theory of gelelectrophoresis of D N A. BRUNO H. ZIMM AND STEPHEN D. LEVENEDepartment of Chemistry, University of California (San -
doi:10.1016/j.jcis.2006.12.075
www.damtp.cam.ac.uk/user/gold/pdfs/teaching/ufk_papers/electrokinetics/hunter.pdf4 Oct 2010: Substitution of this expression for Dud. in Eq. (16) yields. (20)Du = 2κa. ... a) Calculate κa for the suspension.(b) If κa 1 (κa > 20, say), we are in the large κa regime,. -
Lect13.dvi
www.damtp.cam.ac.uk/user/na/PartIB/Lect13.pdf18 Feb 2010: We deduce that l2 =. [. 01. ]. , D2,2 = 3 and A =. [. 1 02 1. ] [. 2 00 3. ] [. 1 20 1. ]. Symmetric positive definite matrices Recall: A is positive definite if xAx > 0 -
MECHANICAL PROCESSES IN BIOCHEMISTRY Carlos Bustamante,1–3 Yann R.…
www.damtp.cam.ac.uk/user/gold/pdfs/teaching/ufk_papers/optical_tweezers/bustamante2004.pdf30 Oct 2010: linke. d)6. 7g85. 20. (at. 300. nm/s. )0. 634. 10. 4. ... Are there other conditions under whichthe equality of Equation 20 may not hold? -
USING STANDARD SYSTE
www.damtp.cam.ac.uk/user/gold/pdfs/teaching/ufk_papers/electrokinetics/viovy.pdf4 Oct 2010: Electrophoresis of DNA and other polyelectrolytes:Physical mechanisms. Jean-Louis Viovy. Laboratoire Physico-Chimie Curie (UMR CNRS 168), Institut Curie Section de Recherche,26 Rue d’Ulm, 75230 Paris cedex 05, France. The dramatic recent advances -
REVIEW OF SCIENTIFIC INSTRUMENTS VOLUME 75, NUMBER 9 SEPTEMBER ...
www.damtp.cam.ac.uk/user/gold/pdfs/teaching/ufk_papers/optical_tweezers/neuman-block.pdf28 Oct 2010: Since their invention just over 20 years ago, optical traps have emerged as a powerful tool withbroad-reaching applications in biology and physics. ... level,9–19 the physics of colloids and mesoscopic systems,20–29 and the mechanical properties of -
Examples2.dvi
www.damtp.cam.ac.uk/user/na/PartIB/Examples2.pdf10 Feb 2010: 20. The s-step Adams–Bashforth method is of order s and has the form. -
PowerPoint Presentation
www.damtp.cam.ac.uk/user/pvl/PLANNING_OUR_FUTURES/baker.pdf26 Sep 2010: 29% 20% 10% 5% 70% 103% 161%. Englandcomparison 16% 13% 7% 43% 54% 115%. ... 0. 20,000. 40,000. 60,000. 80,000. 100,000. 120,000. 140,000. 160,000. 180,000. 200,000. -
Building an understanding of the older population
www.damtp.cam.ac.uk/user/pvl/PLANNING_OUR_FUTURES/brayne.pdf1 Oct 2010: Residential care home n=68 (21%). Care home with nursing n=20 (6%). ... Hospital: NHS long-stay n=11 (3%). 160 46 63 1 3 20 10 2 2 1. -
Tall Buildings in Cambridge – a Public Debate
www.damtp.cam.ac.uk/user/pvl/TALL/pellew_text.pdf23 Mar 2010: Indeed, do we have an agreed vision for how Cambridge should look in 20 or even 50 years time? -
A perspective of GKB's Micro-hydrodynamics
www.damtp.cam.ac.uk/user/hinch/talks/EFMC8.pdf10 Sep 2010: each particle sees a linear flow (local rheology). 20. A startStress system in a suspension of force-free particles JFM 1970. ... V 20 cRa. ). 94. Suspensions: what happened in parallel. -
Statistical Physics Matthew Wingate∗ 3 March 2010 ∗Comment & ...
www.damtp.cam.ac.uk/user/wingate/Notes/StatPhys/fermi.pdf3 Mar 2010: exξ 1. ]dx (9.2.6). 9 FERMI-DIRAC DISTRIBUTION 68. 0 20 40 60 80 100 120x. ... S = FT. V,N. = N kπ2. 2. T. TF. (9.2.20). -
Tall Buildings Seminar – 3 March 2010 (PC Edit ...
www.damtp.cam.ac.uk/user/pvl/TALL/carolin_text.pdf23 Mar 2010: to the university library tower at 48m tall … 20. … -
statphys.dvi
www.damtp.cam.ac.uk/user/wingate/Notes/StatPhys/classical.pdf11 Mar 2010: The integrand in (10.2.6)is the Maxwell distribution (Fig. 20). f (v) = N vD1 exp(. ... Figure 20: The Maxwell distribution. The single particle partition function is then.
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