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Transferrin-Uptake Assay
https://www2.mrc-lmb.cam.ac.uk/groups/hmm/techniqs/Transferrin%20Uptake%20Assay.html21 Feb 2008: serum free medium to cells and incubate at 37 ¡C for the desired time (15-20 minutes for steady state). ... For transferrin alone, use 4¡C. Important: Fix cells with 3.2 % paraformaldehyde pre-equilibrated in serum-free medium at the required -
Dlp Purification:
https://www2.mrc-lmb.cam.ac.uk/groups/hmm/techniqs/Eva%20Methods.htm6 Feb 2008: 1: 5 000. poly. Endophilin1. Ra 37. poly. Eps15. Santa Cruz (C-20). ... Five to 20 cells were co-plated with K. aeorogenes on SM agar plates. -
Sup Fig 1
https://www2.mrc-lmb.cam.ac.uk/groups/hmm/publica/YopJSup.pdf11 Nov 2008: phospho IκB. IκB. YopJ-wt. TNF-α (min) 0 5 10 15 20 0 5 10 15 20. ... YopJ-C172A YopJ-wt. 0 5 10 15 20 0 5 10 15 20 TNF-α (min). -
HARVEY T McMAHON CV
https://www2.mrc-lmb.cam.ac.uk/groups/hmm/people/CV2008c.htm28 Jul 2008: EMBO J. 13, 5051-5061 (1994). 20. McMahon, H.T. and SŸdhof, T.C. ... Biochemical Journal 414, 189-194. Highlights of 20 papers above marked with an asterix. -
www.sciencemag.org/cgi/content/full/1164346/DC1 Supporting Online…
https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/Salje%20et%20al%20Science%202009%20Suppl.pdf15 Dec 2008: nitrogen cooling, FEI company, Eindhoven, NL) equipped with a Gatan energy filter (filter bandpass 20 eV). ... 18. Figure S4 APlasmid-free control. 19. Figure S4 BPlasmid-free control. 20. -
PII: 0304-3991(84)90197-9
https://www2.mrc-lmb.cam.ac.uk/groups/nu/pdf/ultram84.pdf26 Oct 2008: Prior to transfer the bag is sealed and dry nitrogen gas is flushed through it for about 20 s. -
molcel2806mmc1
https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/FtsK%20MolCell%202008%20Lowe%20Supp%20data.pdf16 Jul 2008: ASCII85EncodePages false /AllowTransparency false /AutoPositionEPSFiles true /AutoRotatePages /All /Binding /Left /CalGrayProfile (Dot Gain 20%) /CalRGBProfile (sRGB IEC61966-2.1) /CalCMYKProfile (U.S. -
DOI: 10.1126/science.1165322 , 1710 (2008); 322Science et al.Rachel…
https://www2.mrc-lmb.cam.ac.uk/groups/rlw/download/publications/19008417.pdf18 Dec 2008: F) The Saci1373MIM2 (red)/Saci1372 MIT(yellow) interaction isclosely relatedin structurewith the CHMP6 MIM2(green, extended)/VPS4AMIT (gray helices) inter-action (20). ... 19. M. D. Stuchell-Brereton et al., Nature 449, 740 (2007).20. C. -
No Job Name
https://www2.mrc-lmb.cam.ac.uk/groups/hegde/download/49_MacKinnon_AL_JACS_2007.pdf30 May 2008: described)1 were incubated with or without 2 (500 nM) and 3 (0–20 µM, final. ... 20 mL) and the combined organic fractions were washed with saturated NaCl solution. -
npgrj_nchembio_117 691..699
https://www2.mrc-lmb.cam.ac.uk/groups/rlw/download/publications/18849971.pdf16 Oct 2008: 0 25 50 75 100. Seg1. e. 0.02. 0.2. 2. 20. ... PI-103PP121DMSO PP1. pTyr. 20 5.01. 250. 310. 08. pAkt (308). pS6pAkt (473).
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