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2 Jun 2004: glass coverslips in 24-well plates were infected with trans-fected parasites and were processed for IF 20 –24 h post-transfection asdescribed (32). ... After 20 min on ice, themembrane fraction was isolated by centrifugation (100,000 rpm for 20min in a

15 Apr 2004: Amersham) in 20 mMNa-Phosphate, pH 6.0, 1 mM EDTA, 1 mM DTT. ... Residues 5–75 0.62 0.15 Å(All heavy atoms). Residues 5–75 1.20 0.17 Å.

1 May 2004: The best-characterizedsystem in this context is Spo0J fromB. subtilisand 8–10 copies ofcis-actingparSelements situated around 20% of theoriC region (86). ... 20. Cordell SC, L owe J. 2001. Crystal struc-ture of the bacterial cell division regulatorMinD.

1 Nov 2004: Cells were resuspended in sonication buffer (20 mM Tris [pH 8.0],the Vps25 subunit. ... 20 mM Tris [pH 7.4], 100 mM NaCl, and 2 mM DTT).

20 Sep 2004: but a sizeable fraction (20%) of the E1158Q mutantcomplex eluted with a retention volume consistent with Scc1-C Is a Winged Helix Domain. ... Endogenous Scc1-myc18 wasdetectable 20 min after release from G1 arrest.(B) FACScan analysis shows that cells

29 Dec 2004: Tirnernin) 30 40. B z a’ P ' X- 60. 3, Lc; 5: 40 nei '-YE 20 z. ... A, au- toradiograph of 7.5% SDS PAGE of membrane proteins (20 rg) from each condition.

24 Sep 2004: Native protein was expressed in C41(DE3) cells. Cells were resuspended in buffer A (20 mM Tris, pH 8.0, 50 mMpotassium phosphate, pH 8.0, and 100 mM NaCl) and ... Fractions con-. taining Vps23 UEV were pooled and diluted with an equal volume ofbuffer C

23 Nov 2004: The maximum was selected and each data point was binned and normalised onto a 4-point scale 0 - 20, 20.1 - 40, 40.1 - 60, 60+. ... 5.0 0.4. Amph-P1 FEDNFVP 20.9 2.2. 1.2 0.1 4.83e4 5.1e3. -6.9 0.2.

23 Nov 2004: www.sciencedirect.com Current Opinion in Cell Biology 2004, 16:379–391. G-proteins are required for membrane recruitment[18,20,30,31]. ... L. J Cell Biol 1964, 20:313-332. 2. Pearse BM: Coated vesicles from pig brain: purification andbiochemical

15 Jan 2004: The mouse 139A prion strain, originallyisolated after more than 20 passages in mice, was obtained from R. ... In the brains of Tg196 mice in-oculated with prions from Tg2866 mice, sPrPSc(P101L) ac-counted for 20% of total PrP.