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  2. 73687_Trans

    https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/emboj201099s2.pdf
    28 Apr 2010: Overlap starts appox. at the beginning of the coiled coil (around residue 72) and ends around residue 90, hence 20 amino acids overlap.
  3. Morpheus stock solutions poster portrait A2

    https://www2.mrc-lmb.cam.ac.uk/groups/JYL/WWWrobots/PDF/PresentationsOther/Morpheus_stock_solutions_poster.pdf
    1 Jun 2018: Buffer system 3 MORPHEUS I & III MD2-100-102 Two stocks: 1 M bicine (acid, pKa 8.4) and 1 M Trizma-base (pKa 8.20).
  4. Bacterial actin: architecture of the ParMRC plasmidDNA partitioning…

    https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/ParMRC%20Salje%202008%20EMBO.pdf
    4 Aug 2008: least 20 ParR C-terminal tails present, producing a very high. local concentration. ... Under these condi-. tions, rings of roughly 20 nm diameter could be observed.
  5. No Job Name

    https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/MreC%20MM%202006.pdf
    7 Dec 2006: to polyvinylidene difluoride membrane for3.5 h at 20 mAmps using a wet transfer apparatus (Trans-BlotTMCell, Bio-Rad). ... Genes Dev 20: 113–124. Kunst, F., and Rapoport, G. (1995) Salt stress is an environ-mental signal affecting degradative enzyme
  6. DOI: 10.1126/science.1256917, 963 (2014);346 Science et al.Thomas G.…

    https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/Science-2014-Gligoris-963-7.pdf
    21 Nov 2014: Struct. Mol. Biol. 20, 371–379. (2013).9. C. H. Haering et al., Nature 454, 297–301 (2008).10. ... Ivanov, K. Nasmyth, Mol. Cell 27, 300–310. (2007).20. C. H. Haering et al., Mol.
  7. doi:10.1016/j.molcel.2006.07.020

    https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/HiroseMC2006.pdf
    8 Sep 2006: of the motor domain is rotated by 20 betweenthe ADP and AMPPNP states. ... 1 mM DTT, 0.5 mM GTP, and 10% DMSO), stabilized with 20 mM.
  8. No Job Name

    https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/vandenent2008ftsq.pdf
    4 Mar 2008: enterocolitica FtsQ, in 20 mM Tris,1 mM EDTA, 1 mM NaAzide, 50 mM NaCl, pH 7.0 (TEN50)for S. ... aureus FtsQ and in 20 mM Tris, 1 mM EDTA, 1 mMNa-azide, pH 7.5 (TEN7.5) for C.
  9. BIO CHEM ISTR Y Structure of the SARS-CoV-2 RNA-dependent ...

    https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/SARS%20polym%202021%20e2021946118.full.pdf
    29 Aug 2021: Edited by Eva Nogales, University of California, Berkeley, CA, and approved January 11, 2021 (received for review October 20, 2020). ... All data were processed in RELION 3.1 (20), using particle picks importedfrom crYOLO 1.5 (21) (SI Appendix, Fig.
  10. Jason William Chin Page 1 of 18 MRC Laboratory ...

    https://www2.mrc-lmb.cam.ac.uk/ccsb/wp-content/uploads/2024/01/Chin-cv-26012024.pdf
    29 Jan 2024: K. Lang, J.W. Chin. ACS Chem. Biol. 2014. 9: 16-20. (73) Genetically encoded light activated transcription for spatiotemporal control of gene expression and gene silencing in mammalian cells. ... News. Oct 11 2010 , Chem. Eng. News (Highlights of the Year
  11. elifesciences.org van den Ent et al. eLife 2014;3:e02634. DOI: ...

    https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/mrebdouble2014.pdf
    6 Jun 2014: Scale bar: 20 nm.DOI: 10.7554/eLife.02634.008. Biochemistry | Biophysics and structural biology. ... Bar: 200 nm. (B) Cryo-EM image of a lipid tube. Bar: 20 nm.
  12. Crystal Structures of Bacillus subtilis Lon Protease

    https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/londumanjmb2010.pdf
    20 Jul 2010: 2 Crystal Structures of B. subtilis Lon Protease. 20 amino acids are created.3 These discrete functionsare facilitated by the presence of separate modules,which are responsible for substrate recognition, ATPhydrolysis, ... coli Lon6. and then confirmed
  13. Articleshttps://doi.org/10.1038/s41594-019-0196-z 1MRC Laboratory of…

    https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/Burmann%202019%20NSMB.pdf
    4 Mar 2019: 20 nm. 20 nm. 20 nm. 20 nm. 20 nm. 20 nm 20 nm 20 nm. ... 20 nm 20 nm 20 nm20 nm. 20 nm 20 nm 20 nm.
  14. 3747_0_supp_7_1052456676

    https://www2.mrc-lmb.cam.ac.uk/groups/wschafer/christian.pdf
    9 May 2003: least two chemosensory neurons (AWA and AWC) has been measured to -20 mV, which. ... membranes. After 15-20 minutes of equilibration the cells were transferred into fresh.
  15. DOI: 10.1126/science.1184429 , 1638 (2010); 327Science et al.Simon…

    https://www2.mrc-lmb.cam.ac.uk/groups/rlw/download/publications/20339072.pdf
    2 Aug 2010: hAtg14/Barkor (19, 20) and, in a separate complex,ultraviolet irradiation resistance–associated gene(UVRAG) (21) and Bax-interacting factor-1 (Bif-1)(22). ... predators (19, 20). Similarly, plant growth strat-egies influence tolerance to herbivory (21,
  16. 3919Commentary Introduction Kinesin motors use energy supplied by ATP …

    https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/JCScommentary.pdf
    31 Oct 2007: Apart from the movement of the necklinker, the most striking difference between the ADP and ATP-like states, a 20 rotation of the core domain relative to 4, isalso evident in ... EMBO J. 20, 2611-2618. Yun, M., Bronner, C. E., Park, C.
  17. molcel4221mmc1

    https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/MolCell2012MipZ/mipz2012supp.pdf
    15 Mar 2012: 5 µM) in buffer BC containing the indicated nucleotides (1 mM) and no (F) or 20 mM (G) EDTA. ... Crystallization was observed in three buffers, which all contained ATPγS and 100 mM Tris (pH 8.5) with 18% - 20% ethanol.
  18. Coot: Model-building tools for Molecular Graphics

    https://www2.mrc-lmb.cam.ac.uk/personal/pemsley/coot/files/presentations/bgu/coot-2018-bgu.pdf
    22 Feb 2018: Slide 20. Slide 21. Slide 22. Slide 23. Slide 24. Slide 25.
  19. 201010176 1..6

    https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/PNAS-2010-Aylett-TubZ.pdf
    21 Oct 2010: The GTPase domains of Methanococcus jannaschii FtsZ (19)and bovine α-tubulin (20) superimpose well with TubZ, whereastheir C-terminal domains are a little more divergent. ... Nat Struct Mol Biol 11:1243–1250. 20. Löwe J, Li H, Downing KH, Nogales E
  20. Model-Building with Coot Paul EmsleyUniversity of Oxford June 2011 ...

    https://www2.mrc-lmb.cam.ac.uk/personal/pemsley/coot/web/aca-2012-low-resolution-scripting-ligands-and-bumps.pdf
    28 Jul 2012: O2A PA O3A PB 60.005 20.000 1 ADP var_2 PA O3A PB O1B 59.979 20.000 1 ADP var_3 O2A PA "O5'" "C5'" -59.942 ... O4'" "C1'" 150.000 20.000 1 ADP var_7 "C5'" "C4'" "C3'" "C2'" -150.000 20.000 3.
  21. Nicotinic Acetylcholine Receptor at 4.6 Å Resolution: Transverse…

    https://www2.mrc-lmb.cam.ac.uk/groups/nu/pdf/jmb99.pdf
    15 Sep 2003: Thus with asingle image, there was too much noise for few, ifany, of the layer-lines to be distinguished convin-cingly beyond a resolution of about 20 AÊ. ... Common to all views are the 20 AÊwide vestibules on either side of the membrane,and a more
  22. bc219914979p

    https://www2.mrc-lmb.cam.ac.uk/groups/rlw/download/publications/10329700.pdf
    20 Apr 2002: temperature,and 4-ml aliquots were analyzed by electrophoresis on native 20% poly-acrylamide Phast gels (Amersham Pharmacia Biotech). ... W. (1981) Biochemistry 20, 1932–193832. Irvine, R. (1998) Curr. Biol. 8, R557–R55933.
  23. Coot Updates Paul EmsleySept 2016 Coot 0.8.4 Released● Patterson ...

    https://www2.mrc-lmb.cam.ac.uk/coot/ligands/2016/emsley-ccp4-ligand-meeting-sept-2016-v5.pdf
    28 Sep 2016: Slide 18. Slide 19. Slide 20. Slide 21. Slide 22. Slide 23.
  24. REPORT◥ PROTEOSTASIS UBE2O is a quality control factor fororphans ...

    https://www2.mrc-lmb.cam.ac.uk/groups/hegde/download/Science_2017b.pdf
    4 Aug 2017: S1 to S11References (30–41). 20 February 2017; accepted 8 June 201710.1126/science.aan0178. ... EDTA (Roche) for 20 min and lysed with 26G needle attached to a syringe.
  25. Crystal Structure of the SMC Head Domain: An ABC ATPase with 900…

    https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/TmSMChd%20JMB%202001.pdf
    20 Feb 2001: 20 % (w/v) glycerol,200 mM NaCl, and 0.004 % (v/v) DMSO. ... Trends Biochem. Sci.20, 478-480. Hopfner, K. P., Karcher, A., Shin, D.
  26. tutorial-embo.dvi

    https://www2.mrc-lmb.cam.ac.uk/personal/pemsley/coot/files/tutorial-embo.pdf
    8 Sep 2015: 20. • You can display the contacts too, as you do this:. –
  27. 1256917combinedsupplement

    https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/Gligoris-SM.pdf
    14 Nov 2014: Nat. Struct. Mol. Biol. 20, 371–379 (2013). Medline doi:10.1038/nsmb.2488. 9. C. ... Mol. Cell 27, 300–310 (2007). Medline doi:10.1016/j.molcel.2007.07.002. 20. C. H. Haering, D.
  28. Coot Tools for Cryo-EM Paul EmsleyMRC Laboratory of Molecular ...

    https://www2.mrc-lmb.cam.ac.uk/personal/pemsley/coot/files/presentations/coot-cryoEM-2018-Oct-TF.pdf
    8 Oct 2018: Slide 16. Slide 17. Slide 18. Slide 19. Slide 20. Slide 21.
  29. IntroductionThe endosomal system functions as an internal…

    https://www2.mrc-lmb.cam.ac.uk/groups/hmm/publica/SNX2Cullen2005.pdf
    17 Jan 2006: Here, full-length recombinant SNX2 wasincubated with liposomes comprising phosphatidylcholine(PtdCho), phosphatidylserine (PtdSer) andphosphatidylethanolamine (PtdEth) (each at 26.6% (w/w)) towhich 20% (w/w) of the relevant phosphoinositide was added
  30. PII: S0969-2126(02)00801-8

    https://www2.mrc-lmb.cam.ac.uk/groups/hmm/publica/Kent_McMahonOwen2002.pdf
    30 Jul 2002: Wilson plot B (Å2 ) 20 20. ... A (0.2 M NaCl, 20 mM HEPES [pH 7], 4 mM -mercaptoetha-nol) containing 0.3 M imidazole.
  31. The structure of bactofilin filaments reveals their mode of membrane…

    https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/Deng2019Natmicro.pdf
    26 Aug 2019: 80. 85. 90. 95. 5. 15. 5. 20. 15 80 85 90 95. ... in a 45 Ti rotor (Beckman) for 30 min at 4 C, followed by another centrifugation at 20,000 r.p.m.
  32. PII: S0166-2236(03)00131-0

    https://www2.mrc-lmb.cam.ac.uk/groups/hegde/download/25_Hegde_RS_TINS_2003.pdf
    30 Jun 2003: EMBO J. 20, 5383 – 5391. 12 Ma, J. and Lindquist, S.
  33. The structural basis of novel endosome anchoringactivity of KIF16B ...

    https://www2.mrc-lmb.cam.ac.uk/groups/rlw/download/publications/17641687.pdf
    23 Jul 2007: 40. 80. 120. 0 20 40 60. Req. (R. U). PX concentration (nM). ... layer to the level of its penetration into POPC/POPE (80:20). monolayer.
  34. The patterned assembly and stepwise Vps4-mediated disassembly of…

    https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/Hurtig%202023%20sciadv.ade5224.pdf
    16 Jul 2023: ImmunolabelingFixed cells were rehydrated in PBSTA (phosphate-buffered salinesupplemented with 0.2% Tween 20 and 3% bovine serumalbumin). ... Images were acquiredwith 200-ms exposure time for labeled proteins and 500-ms expo-sure time for DNA stains,
  35. 7400749.qxd

    https://www2.mrc-lmb.cam.ac.uk/groups/hegde/download/41_Ashok_A_EMBORep_2006.pdf
    19 Jun 2006: Annu Rev Cell Dev Biol 20:395–425. Seeger H, Heikenwalder M, Zeller N, Kranich J, Schwarz P, Gaspert A, Seifert B, Miele G, Aguzzi A (2005) Coincident scrapie infection andnephritis
  36. Biallelic Variants in ASNA1, Encoding a Cytosolic Targeting Factor of …

    https://www2.mrc-lmb.cam.ac.uk/groups/hegde/download/CircGen_2019.pdf
    1 Dec 2019: Both missense variants were predicted to be del-eterious (CADD >20 and M-CAP >0.025). ... buffer supplemented with 20 mM imidazole, and eluted with lysis buffer supplemented with 250 mM.
  37. 088542u047

    https://www2.mrc-lmb.cam.ac.uk/groups/hmm/publica/Owen_McMahon1999.pdf
    17 Mar 2003: presence of 20 ml 50% slurry of glutathione-following induction with 0.2 mM IPTG. ... Cell Biol. 20, 313–332.pathway in viral infection. EMBO J. 17, 4585–4593.
  38. Interactive Tools forCryo-EM Map Fitting Paul EmsleyMRC Laboratory of …

    https://www2.mrc-lmb.cam.ac.uk/personal/pemsley/coot/files/presentations/coot-cryoEM-2018-June-PSI.pdf
    19 Jun 2018: Slide 19. Slide 20. Slide 21. Slide 22. Slide 23. Slide 24.
  39. Reconstitution of a prokaryotic minus end-trackingsystem using TubRC…

    https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/PNAS-2015-Fink-E1845-50.pdf
    27 Apr 2015: This effect explained the previouslysuggested stabilization effect of TubRC on filament formation(17, 20). ... For end tracking, 8 nM unlabeledTubR and 20 pM Atto647-labeled tubC were tubC used.
  40. Model-Building with Modern Coot Paul Emsley Scripps, Feb 2019 ...

    https://www2.mrc-lmb.cam.ac.uk/personal/pemsley/coot/files/presentations/Coot-Scripps-Feb-2019-EOS.pdf
    21 Feb 2019: Slide 17. Slide 18. Slide 19. Slide 20. Slide 21. Slide 22.
  41. Structural analysis of the ParR/parC plasmidpartition complex Jakob…

    https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/MollerJensen_ParR_EMMBO_2007.pdf
    4 Oct 2007: 2007 European Molecular Biology Organization The EMBO Journal VOL 26 | NO 20 | 2007. ... 2007 European Molecular Biology Organization The EMBO Journal VOL 26 | NO 20 | 2007 4415.
  42. THE JOURNAL OF BIOLOGICAL CHEMISTRY 0 1990 by The ...

    https://www2.mrc-lmb.cam.ac.uk/groups/hegde/download/1_Pewitt_EB_JBC_1990a.pdf
    29 Dec 2004: the saturable component of bumetanide binding, Keq = k-l/k1 = 20 nM, was determined. ... 1 m. 0 IO 20 30 40. Time (min). 0 10 20 30 40 50 60 70 80.
  43. ANALYSIS Integrating molecular and network biologyto decode…

    https://www2.mrc-lmb.cam.ac.uk/groups/hmm/publica/Schmid_Nature2007.pdf
    22 Aug 2007: Such attachments can be used in manydiverse pathways (for example, actin polymerization associated withvesicle scission and motility18–20). ... Nature Cell Biol. 4,691–698 (2002). 20. Kaksonen, M., Toret, C. P. &
  44. pq240013132p

    https://www2.mrc-lmb.cam.ac.uk/groups/hegde/download/16_Wilhelm_JE_PNAS_2000.pdf
    10 Nov 2000: Recently,two RNA binding proteins, verayVgRBP and VgRBP60, thatbind specifically to the Vg1 localization element have beenidentified (20 –22). ... 1992) Science 255, 991–994.20. Cote, C. A., Gautreau, D., Denegre, J.
  45. REPORT◥ PROTEIN HOMEOSTASIS Mechanistic basis for a moleculartriage…

    https://www2.mrc-lmb.cam.ac.uk/groups/hegde/download/Science_2017.pdf
    19 Jan 2017: TRC40x SGTA -x TRC40 -. time (sec): 0 10 20 30 45 60 300. ... Cell Sci. 127,. 4728–4739 (2014).20. P. Leznicki, S. High, Proc. Natl.
  46. The ER membrane protein complex promotes biogenesis of sterol-related …

    https://www2.mrc-lmb.cam.ac.uk/groups/hegde/download/JCS_2019.pdf
    18 Jan 2019: A) WT and ΔEMC6 (Δ6) cells were exposed to Chol:MBCD (25, 37.5 and50 µM, 20 h) and visualised by staining with Crystal Violet. ... or without 10 µM avasimibe (AVA, 20 h)and visualised by staining with Crystal Violet.
  47. The ER membrane protein complex is a transmembrane domain insertase

    https://www2.mrc-lmb.cam.ac.uk/groups/hegde/download/Science_2017c.pdf
    27 Jan 2018: TA proteins were translated for 20 minutes at 32 C unless otherwise indicated. ... The insertion reaction proceeded at 32 C for 20 minutes unless otherwise stated.
  48. Asgard archaea shed light on the evolutionary origins of the…

    https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/Hatano%20Nat%20Comms%202022.pdf
    13 Jun 2022: Supplementary Fig. 1). These proteins act in sequence to bind,deform and cut membranes during membrane trafficking2–5, celldivision19,20, viral egress21,22 and in other important topologicallysimilar membrane remodelling events ... 150. 50. 250. mAU.
  49. PII: S0304-3991(97)00070-3

    https://www2.mrc-lmb.cam.ac.uk/groups/nu/pdf/ultram97.pdf
    4 Apr 2003: The signal retention improved by only 10% at low resolution (20 A), but by progressively greater amounts at higher resolutions, up to - 40% at 9 8. ... 20]; variable twist has been corrected by ap- plying an innovative real-space algorithm in a study of
  50. Supplementary Text & Figs

    https://www2.mrc-lmb.cam.ac.uk/groups/hmm/publica/Schmid_Nature2007Sup.pdf
    22 Aug 2007: These data come from the following papers: (AP2, clathrin)1,20, (dynamin, clathrin)21,22,. ... endocytosis in Drosophila. Genes Dev. 15, 2967-2979 (2001). 20. Rappoport, J.
  51. x96255u149

    https://www2.mrc-lmb.cam.ac.uk/groups/rlw/download/publications/11090628.pdf
    20 Apr 2002: CCP4,20), and four times with 50 ml of solution 3 (20 mM Tris [pH 7.5], 1994). ... of phosphatidylinositol- 3-kinase. Cancer Res. 54, 2419–2423.Med. Res. Rev. 20, 28–57.

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