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8 Jun 2006: analytical ultracentrifuge with an An60-Ti rotor, with 39, 78 and 156 µM in 150 mM NaCl, 20. ... at 40 and 150 µM protein in 150 mM NaCl, 20 mM HEPES pH 7.4, 2 mM DTT unless.

30 Apr 1999: Undecoratedtubules. t=20 s t=5 min. on tubules on tubules on tubules. ... Results are the average of three experiments s.e.m. 50 10 15 20 25 300.0.

6 Sep 2010:  use robot or manually dispense 20 µl of silicon oil (provided) into each well.

31 Jan 2020: J. Biol. Chem. 293, 4860–4869 (2018). 20. Yang, S.-X., Pollock, H. ... equili-brated with 20 mM Hepes, 200 mM NaCl at pH 7.5.

5 Jan 2005: dendrites. Tuba [20], anotherBAR protein, has Rho GEF activity and multiple SH3 domains that binddynamin, N-WASP (neural Wiskott–Aldrich syndrome protein) and otheractin-regulating proteins.

18 Jun 2018: 20. Figure 5: Coot’s Go To Atom Window (it doesn’t look exactly like this anymore). •

13 Sep 2000: The protein was stored at4 C in 20 mM TRIS, 1 mM EDTA, 1 mM Na-azide. ... P3 yields exclu-sively empty tubes. All reactions were incubated for 20 min at37 C.

11 Apr 2024: tumour suppressor with the membrane of polarized mammalian cells. EMBO J 20, 5929-5939. ... EMBO J 20, 4935-4943. 73. Hamada F & Bienz M (2002) A Drosophila APC tumour suppressor homologue functions in.

15 Jul 2016: 8 % v/v PEG 550 MME1-36 8 % w/v PEG 20,000/ 0.1 M Sodium acetate 5.5 0.2 M Potassium thiocyanate. ... 20 % v/v Glycerol2-47 1.6 M Sodium/potassium phosphate 6.02-48 1 M Sodium citrate 6.2.

18 Dec 2022: S2A). ABC-typeATPases bound to ADP.BeF3 often represent the enzyme–substrate complex (20). ... The peak fractionswere concentrated using a Vivaspin 20 100,000-Da centrifugal concentrator andfrozen.

29 Sep 2017: 19. Puzzle 5-2: Annealed. 20. Puzzle 5-2: Comparison. 21. Puzzles 5-2: Coot model is better? ... Slide 19. Slide 20. Slide 21.

16 Jul 2003: and POPC/POPE/phos-phatidylinositol 3,4,5-trisphosphate (77:20:3) vesicles. ... a Increase in Kd relative to binding POPC/POPE/PtdIns(4,5)P2 (77:20:3) vesicles.b Not measurable.

16 Aug 2004: Mol. Biol. (2004) 341, 839–852. diverse roles18–20 that include anchoring the ring tothe cell membrane, invagination and constriction,septum formation and peptidoglycan synthesis toyield two separate daughter cells. ... After centrifugation, the

10 Sep 1999: We have usedboth secondary structure [19] and coiled-coil predictionprograms [20] to define a domain (residues 1–227), whichdoes not contain residues with significant coiled-coil prob-ability, but includes all ... S1 S2.

12 Nov 2001: FtsZ is highly conserved and is present in mostbacteria and archaea [19,20]. ... FtsA is the keycomponent in the sequential recruitment of othercomponents of the divisome [19,20].

6 Jul 1999: Crystallography; CHC proximal legdomain residues 1210 to 1516 (20). 2.6 CHC proximal leg, clathrin LC. ... 20. J. A. Ybe et al., Nature 399, 371 (1999).21. Single-letter abbreviations for the amino acid resi-.

20 May 2010: 1 20 73 125 164. N-terminal domain. C-terminal domain. Figure 1 B. ... ω. Cβ. 10 20 30 40 50 60 70–8–40. 48. Δω.

8 Dec 2007: Concentration (µM)0 20 40 60 80 100. I–I 0. (arb. itrar. ... interaction. Indeed, CHMP1Bbinds human Vps4A MIT domain with a Kd of 20 mM (ref.

31 Aug 2018: Under “Weight Matrix”, set the Refinement Weight to 20. 1.2. Automatic model building will only rarely produce a model which is both complete and correct.

21 Mar 2003: whereas glutamate release was essentially unaffected (96 9% ofmaximal; n = 3) in the presence of 20 µM cyclosporin A. ... Fol-lowing two washes, the synaptosomes were spun briefly a third timeand resuspended in lysis buffer (20 mM Hepes).

7 Mar 2019: Upon deletion of the CTD (construct C), GCN2’sbasal activity increased 20-fold (Fig. ... 0.001 0.01 0.1 1 10 1000. 10. 20. 30. 40. Time (min). %

23 Jan 2012: n d for a s e c o n d image a t 20,000 A u n - derfocus. ... and the second one at ,20,000 A underfocus, using a nominal magnification of 36,000.

1 Jun 2010: 1), with more than 20 established binding part-ners. AP-2 therefore acts as an organizational scaffold that associates with several partners synchronously. ... Mol. Biol. Cell 20, 4640–4651 (2009).4. Boettner, D. R. et al.

10 Dec 2014: neuron, which appears to be unimpor-tant for touch-evoked escape behavior, expresses the neuro-peptide precursor gene flp-20 [43], which has recently been. ... elegans. Learn Mem 20(2):103–108. doi:10.1101/lm.028993.112. 52. Li W, Feng Z, Sternberg PW,

25 Nov 2019: A-siteP-site STOP. Testsequence. a. 20 40. Time (min). 600. 20. 40. ... Nat. Rev. Mol. Cell Biol. 20, 406–420 (2019). 5. van Hoof, A. &

17 Mar 2003: vesicle endocytosis. J. Neurosci. 20, 8667–8676. Ye, W., and Lafer, E.M. ... I. Developmental expres-sion and cellular localization of the F1-20 protein and mRNA.

16 Jul 2015: coot.available-comp-id JE 20. JEA JEB JEC JED JEE JEG JEH JEI JEJ JEK. ... Slide 15. Slide 16. Slide 17. Slide 18. Slide 19. Slide 20.

6 Apr 2017: The central pore is 20 Å wide, and the lattice is70 Å thick. ... J. Bacteriol. 192, 5855–5865 (2010). 20. Smit, J., Engelhardt, H., Volker, S., Smith, S.

28 Apr 2024: coil (9, 20) (Fig.

14 Nov 2021: cycle have remained unclear [3,19,20]. Here, we have determined the complete three-. ... Curr Res Struct. Biol 1, 13–20.17 Jean NL, Rutherford TJ and Lowe J (2020) FtsK in.

11 Aug 2008: Similar results wereobtained for Arf6, but Arf6 bound even more strongly to mem-branes in the absence of GTP, probably due to binding sites onthe protein core ([19,20] and results ... J. Biol. Chem. 280, 21661–21666. 20 Amor, J. C., Horton, J.

1 Nov 2012: We used 20-30 rats (Sprague-Dawley, Madison, WI), of 160 g body wt, for each set of experiments. ... pieces, and homogenized in 20-ml portions in a Potter-Elvehjem glass homogenizer with a loose-fitting teflon pestle.

30 Jan 2005: Soj ATPase is activated by the N-terminal 20 amino. acids of Spo0J. ... MinE: first 35 amino acids (N-terminal domain). FtsA:last 20 amino acids.

15 Apr 2004: Amersham) in 20 mMNa-Phosphate, pH 6.0, 1 mM EDTA, 1 mM DTT. ... Residues 5–75 0.62 0.15 Å(All heavy atoms). Residues 5–75 1.20 0.17 Å.

16 Oct 2009: trans-SNARE complexes, which have been suggestedto be ordered ringwise around the future fusionsite (20). ... Anestimate of the energy released on going frombuckle to stalk formation is 20 kBT.

23 Dec 2013: A single band at 20 kDa is visible, in agreement with the expected size of the protein. ... 3:532–536. 20. Espeli O, Levine C, Hassing H, Marians KJ. 2003.

8 Dec 2007: 0. 20. 40. 60. 80. 100. 120. 140. 0 50 100 150p85ni (nM). ... 65, 4562 (2005).20. S. J. Isakoff et al., Cancer Res. 65, 10992 (2005).21.

14 Jun 2007: Chem. 279, 36059–36071. 20. Bache, K.G., Slagsvold, T., Cabezas, A., Rosendal, K.R., Raiborg, C. ... and Sundquist, W.I. (2004) Annu. Rev. Cell Dev. Biol. 20, 395–42553.

27 Sep 2022: MME 8.55 % 1-19 PEG 300 25.65 % HEPES 75 mM 7.1 PEG 600 8.55 % 1-20 PEG 350 MME 25.65 % HEPES 75 mM 7.3 PEG ... 15 % 2-19 PEG 600 8.55 % HEPES 75 mM 7.1 PEG 300 42.9 % 2-20 PEG 1000 7.5 % HEPES 75 mM 7.3 PEG 350 MME

20 Apr 2002: Communicated by Max F. Perutz, Medical Research Council, Cambridge, United Kingdom, June 20, 1997 (received for review April 2, 1997). ... 20. Leslie, A. G. W. (1992) Joint CCP4 and ESF-EACMB Newsletteron Protein Crystallography (Daresbury Lab.,

24 Oct 2018: Slide 19. Slide 20. Slide 21. Slide 22. Slide 23. Slide 24.

21 Jan 2010: 20. • You can display the contacts too, as you do this:. – ... Python enabled version only: a dialog with geometric outliers and other in-teresting things (if they exist) detected by Refmac shows up 20.

10 May 2017: 60.005 20.000 1 ADP var_2 PA O3A PB O1B 59.979 20.000 1 ADP var_3 O2A PA "O5'" "C5'" -59.942 20.000 1 ADP ... 20.000 1 ADP var_7 "C5'" "C4'" "C3'" "C2'" -150.000 20.000 3.

1 May 2004: The best-characterizedsystem in this context is Spo0J fromB. subtilisand 8–10 copies ofcis-actingparSelements situated around 20% of theoriC region (86). ... 20. Cordell SC, L owe J. 2001. Crystal struc-ture of the bacterial cell division regulatorMinD.

19 Jun 2003: holes, using a 300-kV field emission microscopeincorporating a top-entry liquid-helium-cooled stage10 (magnification, £36,800; defocusrange, 9,000–16,000 Å; dose, ,20 electrons Å22). ... 3H]diazofluorene. J. Biol. Chem. 273, 8659–8668 (1998). 20.

3 Dec 2004: This points to a function ofAP180 in limiting vesicle size (see also refs 13, 20, 21). ... interactions. J. Cell Biol. 155, 193–200 (2001). 20. Zhang, B. et al.

24 May 2017: For three-. dimensional classification, an initial 20 Å resolution model. was generated by Fourier–Bessel synthesis from structure-.

26 Jan 2006: Dialyze into 150 mM NaCl, 20 mMHEPES pH 7.4, 0.4 mM DTT for use. ... 1998), dialyzed into HKMbuffer, and centrifuged for 20 min at 100,000g immediately prior to useto remove aggregates.

8 Jun 2020: For thedsDNA, a 20-bp B-form dsDNA model was generated in Coot (32) and fittedmanually inside the map. ... Proc. Natl. Acad. Sci.U.S.A. 114, E697–E706 (2017). 20. A. H. de la Peña, E.

14 Aug 2015: 5000 skeletonswere randomly selected from each of 20 randomly selected N2 worms recorded crawling onfood. ... n-gram modelThe n-gram language model analyses were implemented in C and Python using the OpenFST[45] and OpenGRM n-gram [20] libraries.