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MD1-91, MD1-92 and MD1-92-FX Morpheus II 10 mL, HT-96 and FX-96…
https://www2.mrc-lmb.cam.ac.uk/screens2/pdf/LMB20.pdf9 Jul 2018: MD2-100-240. MD2-250-240. 60%Precipitant Mix 7 20% w/v PEG 8000, 40% v/v 1,5-Pentanediol. ... B7 2 mM Divalents II none 30 % v/v Precipitant Mix 71-20 B8 2 mM Divalents II none 31 % v/v Precipitant Mix 81-21 B9 2 mM Divalents II -
Journal of Visualized Experiments www.jove.com Copyright © 2018…
https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/JOVE2018.pdf19 Apr 2018: Then, add 20 L of deionized water to the main container of the liquid handler. ... After use, flushing with water and then 20% v/v ethanol solution prevents microbial growth. -
mbar-v2
https://www2.mrc-lmb.cam.ac.uk/personal/pemsley/coot/files/presentations/mbar-v2.pdf31 Aug 2018: Under “Weight Matrix”, set the Refinement Weight to 20. 1.2. Automatic model building will only rarely produce a model which is both complete and correct. -
Manual_CO-311 JBScreen Solubility HTS
https://www2.mrc-lmb.cam.ac.uk/screens2/pdf/SOLUB.pdf19 Mar 2018: In addition, a positive control for precipitation (20% acetonitrile, well# A1) is included in the screen. -
Cryo-EM reconstruction of AlfA from Bacillus subtilis reveals the…
https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/AlfA2018.pdf20 Mar 2018: The locus codesfor the ParMRC system that, besides ParM, contains the DNA-binding protein ParR, and a centromeric sequence parC (19, 20).Principles governing plasmid segregation by the ParMRC systemare well ... J Mol Biol269:505–513. 20. Popp D, et al. -
tutorial.dvi
https://www2.mrc-lmb.cam.ac.uk/personal/pemsley/coot/files/newt.pdf18 Jun 2018: 20. Figure 5: Coot’s Go To Atom Window (it doesn’t look exactly like this anymore). • -
MD1-93 the Morpheus Additive Screen
https://www2.mrc-lmb.cam.ac.uk/screens2/pdf/Addit-01.pdf5 Jul 2018: A1 Water control -A2 PEG 20000 precipitant 20.00 % w/vA3 PEG 500 MME precipitant 40.00 % w/vA4 PEG 8000 precipitant 20.00 % w/vA5 Ethylene glycol cryoprotectant 40.00 % ... trihydrochloride stabilizer 0.20 MH11 1,4-Diaminobutane dihydrochloride -
Coot: Model-building tools for Molecular Graphics
https://www2.mrc-lmb.cam.ac.uk/personal/pemsley/coot/files/presentations/coot-cshl-2018.pdf24 Oct 2018: Slide 19. Slide 20. Slide 21. Slide 22. Slide 23. Slide 24. -
Morpheus stock solutions poster portrait A2
https://www2.mrc-lmb.cam.ac.uk/groups/JYL/WWWrobots/PDF/PresentationsOther/Morpheus_stock_solutions_poster.pdf1 Jun 2018: Buffer system 3 MORPHEUS I & III MD2-100-102 Two stocks: 1 M bicine (acid, pKa 8.4) and 1 M Trizma-base (pKa 8.20). -
MD1-118 Hippocrates additive screen
https://www2.mrc-lmb.cam.ac.uk/screens2/pdf/Addit-03.pdf9 Jul 2018: C1 13 9 % Pyridoxine hydrochloride C2 14 9 % Thiamine hydrochloride C3 15 9 % CytidineC4 16 6 % Inosine†C5 17 6 % RibavirinC6 18 6 % ThymidineD1 19 6 % UridineD2 20 1.5 % -
MD1-116-117 Morpheus III-Morpheus III HT-96
https://www2.mrc-lmb.cam.ac.uk/screens2/pdf/LMB22.pdf9 Jul 2018: Catalogue Number (250 mL). 60% Precipitant Mix 1 40% v/v PEG 500 MME; 20 % w/v PEG 20000. ... MD2-100-82 MD2-250-82. 60% Precipitant Mix 3 40% v/v Glycerol; 20% w/v PEG 4000. -
MD1-46, MD1-47 and MD1-47-FX Morpheus 10 mL, HT-96 and FX-96…
https://www2.mrc-lmb.cam.ac.uk/screens2/pdf/LMB17.pdf9 Jul 2018: 60% Precipitant Mix 1 P500MME_P20K 40% v/v PEG 500 MME; 20 % w/v PEG 20000. ... MD2-100-82 MD2-250-82. 60% Precipitant Mix 3 GOL_P4K 40% v/v Glycerol; 20% w/v PEG 4000. -
Coot: Model-building tools for Molecular Graphics
https://www2.mrc-lmb.cam.ac.uk/personal/pemsley/coot/files/presentations/bgu/coot-2018-bgu.pdf22 Feb 2018: Slide 20. Slide 21. Slide 22. Slide 23. Slide 24. Slide 25. -
Coot Tools for Cryo-EM Paul EmsleyMRC Laboratory of Molecular ...
https://www2.mrc-lmb.cam.ac.uk/personal/pemsley/coot/files/presentations/coot-cryoEM-2018-Oct-TF.pdf8 Oct 2018: Slide 16. Slide 17. Slide 18. Slide 19. Slide 20. Slide 21. -
Interactive Tools forCryo-EM Map Fitting Paul EmsleyMRC Laboratory of …
https://www2.mrc-lmb.cam.ac.uk/personal/pemsley/coot/files/presentations/coot-cryoEM-2018-June-PSI.pdf19 Jun 2018: Slide 19. Slide 20. Slide 21. Slide 22. Slide 23. Slide 24. -
Manipulating Ligands Using Coot with a bit of carbohydrates ...
https://www2.mrc-lmb.cam.ac.uk/personal/pemsley/coot/files/presentations/bgu/coot-feb-2018-ligands-carbohydrate-bgu.pdf22 Feb 2018: 60.005 20.000 1 ADP var_2 PA O3A PB O1B 59.979 20.000 1 ADP var_3 O2A PA "O5'" "C5'" -59.942 20.000 1 ADP ... 20.000 1 ADP var_7 "C5'" "C4'" "C3'" "C2'" -150.000 20.000 3. -
Coot Tools for Cryo-EM Paul EmsleyMRC Laboratory of Molecular ...
https://www2.mrc-lmb.cam.ac.uk/personal/pemsley/coot/files/presentations/coot-cryoEM-2018-Aug-Stockholm.pdf15 Aug 2018: Slide 17. Slide 18. Slide 19. Slide 20. Slide 21. Slide 22. -
The ER membrane protein complex is a transmembrane domain insertase
https://www2.mrc-lmb.cam.ac.uk/groups/hegde/download/Science_2017c.pdf27 Jan 2018: TA proteins were translated for 20 minutes at 32 C unless otherwise indicated. ... The insertion reaction proceeded at 32 C for 20 minutes unless otherwise stated. -
15211199233993 1..45
https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/MreBHussain2018.pdf15 Mar 2018: B) Confined cells induced at 3 mM. xylose in 20 mM Mg2+ progressively swell upon escaping confinement into free culture. ... Bottom) BRB785 cells in 20 mM Mg2+ were depleted of Pbp2a by growth in 0 mM IPTG. -
mbar-v2
https://www2.mrc-lmb.cam.ac.uk/personal/pemsley/coot/web/tutorial/model-building-and-refinement-v2.pdf31 Aug 2018: Under “Weight Matrix”, set the Refinement Weight to 20. 1.2. Automatic model building will only rarely produce a model which is both complete and correct. -
Prokaryotic cytoskeletons: protein filaments organizing small cells
https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/nrmicro.2017.153.pdf18 Jan 2018: Nature Reviews Microbiology, (2018). doi:10.1038/nrmicro.2017.153
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