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Protocol for Hamilton LABSTAR
https://www2.mrc-lmb.cam.ac.uk/groups/JYL/WWWrobots/PDF/Manuals/Protocol_for_LabStar.pdf11 Dec 2017: PROTOCOL System 2: setting up crystallization droplets (100 nL protein 100 nL condition) from a single sample in 20 LMB plates 1. ... 15. Clean the SBS lids with a 20% ethanol solution before stacking them on the left-hand side of the liquid handler for -
Model-Building with Coot Adding N-linked Glycosylation& Demo Paul …
https://www2.mrc-lmb.cam.ac.uk/personal/pemsley/coot/files/coot-may-2017-aca-new-orleans.pdf29 May 2017: 60.005 20.000 1 ADP var_2 PA O3A PB O1B 59.979 20.000 1 ADP var_3 O2A PA "O5'" "C5'" -59.942 20.000 1 ADP ... 20.000 1 ADP var_7 "C5'" "C4'" "C3'" "C2'" -150.000 20.000 3. -
Protocol for Tecan EVO
https://www2.mrc-lmb.cam.ac.uk/groups/JYL/WWWrobots/PDF/Manuals/Protocol-for-Tecan-EVO.pdf11 Dec 2017: 3. Then, add 20 L of deionized water to the main container of the liquid handler. ... Disconnect the coupling inserts from the small container (20% ethanol rinsing solution) and connect the inserts to the main container. -
1 Coot Updates Paul EmsleySept 2017 2 N-linked Carbohydrates● ...
https://www2.mrc-lmb.cam.ac.uk/personal/pemsley/coot/files/presentations/Coot-CCP4-Ligands-Sept-2017-v2.pdf29 Sep 2017: 19. Puzzle 5-2: Annealed. 20. Puzzle 5-2: Comparison. 21. Puzzles 5-2: Coot model is better? ... Slide 19. Slide 20. Slide 21. -
Protocol for dragonfly
https://www2.mrc-lmb.cam.ac.uk/groups/JYL/WWWrobots/PDF/Manuals/protocols_for_dragonfly.pdf13 Dec 2017: The advanced setting for ‘max shot vol’ should be lowered from 6,000 to 3,000 when using solutions containing [isopropanol] > 10% v/v and [MPD] > 20% v/v. ... secMAXI 24 200 1.8 2 min 25 secMAXI 48 200 3 4 min 20 sec. -
Comparing AceDRG vs CSD LIGANDS Meeting Sept 2017 Rob ...
https://www2.mrc-lmb.cam.ac.uk/personal/pemsley/coot/files/presentations/2017_Sept_Ligands.pdf29 Sep 2017: 19. AOM : 1lho. AOM (CSD). 20. AOM : 1lho. AOM (CSD). -
Protocol for dragonfly copy
https://www2.mrc-lmb.cam.ac.uk/groups/JYL/WWWrobots/PDF/Manuals/Protocol_mosquito_additive.pdf13 Dec 2017: Rinse the container of condition with deionized water and 20 % ethanol for reuse. ... 5. Seal the cell culture plate containing the screen with an aluminum sheet and place it back in the -20 C incubator. -
Manipulating Ligands Using Coot with a bit of carbohydrates ...
https://www2.mrc-lmb.cam.ac.uk/personal/pemsley/coot/files/coot-may-2017-ligands-madrid-mcs2017-csic.pdf10 May 2017: 60.005 20.000 1 ADP var_2 PA O3A PB O1B 59.979 20.000 1 ADP var_3 O2A PA "O5'" "C5'" -59.942 20.000 1 ADP ... 20.000 1 ADP var_7 "C5'" "C4'" "C3'" "C2'" -150.000 20.000 3. -
Optimization of crystallization conditions with additive screening…
https://www2.mrc-lmb.cam.ac.uk/groups/JYL/WWWrobots/PDF/PresentationsOther/additive-screening.pdf9 Jan 2017: The screens are normally stored at -20 C since they are not used regularly and contain volatile and unstable compounds. -
Pymol guide
https://www2.mrc-lmb.cam.ac.uk/groups/nagai/download/pymolguide.pdf19 Dec 2017: 1026.595825195, 277.550354004, 267.541595459, 277.006958008, 827.363769531, 1226.079711914, -20.000000000 ). Conclusion Hopefully you are now confident to explore the spliceosome using our PyMOL sessions. -
Structure of the hexagonal surface layer on Caulobacter crescentus…
https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/s-layer-nmicrobiol201759.pdf6 Apr 2017: The central pore is 20 Å wide, and the lattice is70 Å thick. ... J. Bacteriol. 192, 5855–5865 (2010). 20. Smit, J., Engelhardt, H., Volker, S., Smith, S. -
REPORT◥ PROTEOSTASIS UBE2O is a quality control factor fororphans ...
https://www2.mrc-lmb.cam.ac.uk/groups/hegde/download/Science_2017b.pdf4 Aug 2017: S1 to S11References (30–41). 20 February 2017; accepted 8 June 201710.1126/science.aan0178. ... EDTA (Roche) for 20 min and lysed with 26G needle attached to a syringe. -
MergedFile
https://www2.mrc-lmb.cam.ac.uk/groups/nu/pdf/iucrj17.pdf24 May 2017: For three-. dimensional classification, an initial 20 Å resolution model. was generated by Fourier–Bessel synthesis from structure-. -
REPORT◥ PROTEIN HOMEOSTASIS Mechanistic basis for a moleculartriage…
https://www2.mrc-lmb.cam.ac.uk/groups/hegde/download/Science_2017.pdf19 Jan 2017: TRC40x SGTA -x TRC40 -. time (sec): 0 10 20 30 45 60 300. ... Cell Sci. 127,. 4728–4739 (2014).20. P. Leznicki, S. High, Proc. Natl. -
Four-stranded mini microtubules formed by Prosthecobacter BtubAB show …
https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/PNAS-2017-Deng-E5950-8.pdf29 Dec 2017: TPR11 helices. 12. 34. 20 nm50 nm. BtubAB micrograph. kinesin light chainPDB 3ZFW. ... Proto-filaments contain alternating A and B subunits, as was suggested(7, 20). -
IL-17 is a neuromodulator of Caenorhabditis elegans sensory responses
https://www2.mrc-lmb.cam.ac.uk/groups/hegde/download/Nature_2017.pdf13 Jan 2017: 1f)20. The ilc-17.1, ilcr-1, and ilcr-2 mutants responded to 21% O2 immediately after being transferred to the. ... 20. 40. 60. 80. 100. 0Sp. eed. (μm. s–1. ) t = 2 h. -
A Polymerization-Associated Structural Switch in FtsZ That Enables…
https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/mBio-2017-Wagstaff-.pdf3 May 2017: It is difficult to explain how the kineticpolarity needed for treadmilling can be coupled to the structural polarity of single-stranded filaments, such as those of FtsZ (20). ... B) GTPase activity of FtsZ proteinsat 10 and 20 M in the presence of GTP or -
doi:10.1016/j.pbiomolbio.2004.07.009
https://www2.mrc-lmb.cam.ac.uk/groups/JYL/WWWrobots/PDF/Publications/Robotic_nanolitre_protein_crystallisation.pdf22 Sep 2017: At the MRC Laboratory of Molecular Biology (LMB) about 15–20 groups are more or lessintensely involved in protein crystallisation and/or structure determination. ... Our initial idea for long-term storage of the plateswas to store them at 20 1C to
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