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23 Oct 2012: crystallization. Crystals were obtained in 20 % PEG 6000, 100 mM Bicine pH 8.6, 1 M LiCl, at 10 mg/ml. ... Isothermal Calorimetry (ITC). ITC experiments were performed using a MicroCal iTC200 machine with a ParM concentration of 20 µM and.

25 Nov 2012: It has been proposed that mutations in the ABDdomain could disrupt interactions with the rest of the catalyticsubunit (12, 20); however, no clear mechanism by which thisconformational shift would activate lipid ... Proc Natl Acad Sci USA 104:5569–5574.

12 Oct 2012: ring-like structures, with a typical diameter of 15–20 nm, mean 17.5, n = 43 (Fig. ... Nature 450:1268–1271. 20. Dam M, Gerdes K (1994) Partitioning of plasmid R1.

5 Dec 2012: The mixture was loaded onto a col-umn cartridge and extensively washed with buffer containing 20 mM im-idazole. ... buffer containing 20 mM Hepes-NaOH (pH 7.7), 100 mM NaCl, and 2 mM TCEP.

27 Mar 2012: The divisome is currently known to. contain more than 20 components and can be divided into. ... Next, the tubes were centri-fuged at 57 k at 20 1C for 30 min.

17 Jan 2012: Received: Dec 2, 2010Revised: Aug 16, 2011Accepted: Sep 20, 2011. These authors contributed equally to this work.The authors declare no competing interests.Address correspondence to: Richard Lundmark (Richard.lundmark@medchem. ... Louis, MO) and used at

23 May 2012: 293T cells expressing. YFP-tagged TCRa were treated with MG132 (20 mM) for 4 hr. ... tions specified in the figure legends in a buffer containing 20 mM HEPES 7.4,.

12 Oct 2012: Cells were fixed in4% paraformaldehyde for 20 minutes at room temperature and stained forTRAP. ... Scale bars,200 nm in panel A and 20 m in panels B through D.

7 Mar 2012: 20) Page 12, line 9-10: Assuming that cell filamentation is worse with the FtsA mutants and assuming that these mutants really are worse at polymerizing than WT FtsA, increased cell ... 20. Page 12, line 9-10: Assuming that cell filamentation is worse