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Model-Building with Modern Coot Paul Emsley Scripps, Feb 2019 ...
https://www2.mrc-lmb.cam.ac.uk/personal/pemsley/coot/files/presentations/Coot-Scripps-Feb-2019-EOS.pdf21 Feb 2019: Slide 17. Slide 18. Slide 19. Slide 20. Slide 21. Slide 22. -
Activation of GCN2 by the ribosomal P-stalk
https://www2.mrc-lmb.cam.ac.uk/groups/hegde/download/PNAS_2019.pdf7 Mar 2019: Upon deletion of the CTD (construct C), GCN2’sbasal activity increased 20-fold (Fig. ... 0.001 0.01 0.1 1 10 1000. 10. 20. 30. 40. Time (min). % -
Current Developments in Coot for Macromolecular Model Building…
https://www2.mrc-lmb.cam.ac.uk/coot/protein-science/Casanal_Lohkamp_Emsley_11112019-pe-2.pdf11 Nov 2019: 20. Although writing multi-threaded code is difficult, the pay-off is substantial. ... Acta Crystallogr. D Biol. Crystallogr. 68:368–380. 20. Schröder GF, Levitt M, Brunger AT (2010) Super-resolution biomolecular crystallography with low-resolution -
Mechanism of ribosome stalling during translation of a poly(A) tail
https://www2.mrc-lmb.cam.ac.uk/groups/hegde/download/NSMB_2019.pdf25 Nov 2019: A-siteP-site STOP. Testsequence. a. 20 40. Time (min). 600. 20. 40. ... Nat. Rev. Mol. Cell Biol. 20, 406–420 (2019). 5. van Hoof, A. & -
Articleshttps://doi.org/10.1038/s41594-019-0196-z 1MRC Laboratory of…
https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/Burmann%202019%20NSMB.pdf4 Mar 2019: 20 nm. 20 nm. 20 nm. 20 nm. 20 nm. 20 nm 20 nm 20 nm. ... 20 nm 20 nm 20 nm20 nm. 20 nm 20 nm 20 nm. -
Biallelic Variants in ASNA1, Encoding a Cytosolic Targeting Factor of …
https://www2.mrc-lmb.cam.ac.uk/groups/hegde/download/CircGen_2019.pdf1 Dec 2019: Both missense variants were predicted to be del-eterious (CADD >20 and M-CAP >0.025). ... buffer supplemented with 20 mM imidazole, and eluted with lysis buffer supplemented with 250 mM. -
Crystal Structures and Nuclear Magnetic Resonance Studies of the Apo…
https://www2.mrc-lmb.cam.ac.uk/groups/JYL/WWWrobots/PDF/Publications/acs.biochem.9b00296.pdf31 Jul 2019: The cells were lysed via sonication, and the lysate wascleared by centrifugation at 18000 rpm for 20 min. ... Dev. 20, 9195.(17) Soucek, L., Whitfield, J., Martins, C. P., Finch, A. -
The structure of bactofilin filaments reveals their mode of membrane…
https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/Deng2019Natmicro.pdf26 Aug 2019: 80. 85. 90. 95. 5. 15. 5. 20. 15 80 85 90 95. ... in a 45 Ti rotor (Beckman) for 30 min at 4 C, followed by another centrifugation at 20,000 r.p.m. -
The ER membrane protein complex promotes biogenesis of sterol-related …
https://www2.mrc-lmb.cam.ac.uk/groups/hegde/download/JCS_2019.pdf18 Jan 2019: A) WT and ΔEMC6 (Δ6) cells were exposed to Chol:MBCD (25, 37.5 and50 µM, 20 h) and visualised by staining with Crystal Violet. ... or without 10 µM avasimibe (AVA, 20 h)and visualised by staining with Crystal Violet. -
TTC5 mediates autoregulation of tubulin via mRNA degradation
https://www2.mrc-lmb.cam.ac.uk/groups/hegde/download/Science_2019.pdf19 Nov 2019: Methods Mol. Biol. 619, 339–363 (2010). doi:10.1007/978-1-60327-412-8_20 Medline. 36. Q. Feng, S. ... Shao, In vitro reconstitution of translational arrest pathways. Methods 137, 20–36 (2018).
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