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MD1-46 Morpheus
https://www2.mrc-lmb.cam.ac.uk/screens2/pdf/LMB23.pdf4 Aug 2021: Catalogue Number (250 mL). 60% Precipitant Mix 1 P500MME_P20K 40% v/v PEG 500 MME; 20 % w/v PEG 20000. ... MD2-100-82 MD2-250-82. 60% Precipitant Mix 3 GOL_P4K 40% v/v Glycerol; 20% w/v PEG 4000. -
Microscopy, 2021, 1–6doi:https://doi.org/10.1093/jmicro/dfab023…
https://www2.mrc-lmb.cam.ac.uk/groups/nu/pdf/mic21.pdf26 Jul 2021: A likely explanation, in line with other structuralevidence [13,20], is that bilayer thickness is modulated primarily bythe properties of the embedded protein, rather than by cholesterol,in regions where the ... Sci. Adv. 7: eabe6204. 20. Norimatsu Y, -
BIO CHEM ISTR Y Structure of the SARS-CoV-2 RNA-dependent ...
https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/SARS%20polym%202021%20e2021946118.full.pdf29 Aug 2021: Edited by Eva Nogales, University of California, Berkeley, CA, and approved January 11, 2021 (received for review October 20, 2020). ... All data were processed in RELION 3.1 (20), using particle picks importedfrom crYOLO 1.5 (21) (SI Appendix, Fig. -
Cryo‐EM structure of the full‐length Lon protease from Thermus…
https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/Lon%20Coscia%202021%20FEBS.pdf14 Nov 2021: cycle have remained unclear [3,19,20]. Here, we have determined the complete three-. ... Curr Res Struct. Biol 1, 13–20.17 Jean NL, Rutherford TJ and Lowe J (2020) FtsK in. -
Single‐dose immunisation with a multimerised SARS‐CoV‐2 receptor…
https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/COVID%20vaccine%202021%201873-3468.14171.pdf29 Aug 2021: vaccines directed against SARS-CoV-2 deliver high. antibody responses in animal models [19,20]. ... Spike-SpyT2. RBD-S-Dps. RBD-SpyT2. SpyC-Dps. 1 : 10. 0. 1 : 20. -
Science Journals — AAAS
https://www2.mrc-lmb.cam.ac.uk/groups/hegde/wp-content/uploads/sites/8/2023/08/Science_2021.pdf26 Aug 2021: complete datasettop 20% abundant. (p < 10-6). (p < 10-6). (p < 10-6). ... Mol. Cell Biol. 20, 116–131. (2019).3. A. Rousseau, A. Bertolotti, Nat. -
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https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/cohesin%20folding%202021%20elife-67268-v1.pdf14 Jul 2021: mately 60% of WT levels, which was further reduced to 20% by scc4D. ... along chromosome arms more modestly than smc1D588Y, raising loading in scc4-4 cells from 20%. -
A unified evolutionary origin for the ubiquitous protein transporters …
https://www2.mrc-lmb.cam.ac.uk/groups/hegde/wp-content/uploads/sites/8/2023/08/BMC_Biology_2021.pdf15 Dec 2021: One, YidC, is found in the prokaryoticplasma membrane [18, 19], whereas the other three areparalogs located in the eukaryotic endoplasmicreticulum (ER): TMCO1 [20], EMC3 [21–24], and GET1[25]. ... By contrast, IMPs with largetranslocated domains became -
The mechanisms of integral membrane protein biogenesis
https://www2.mrc-lmb.cam.ac.uk/groups/hegde/wp-content/uploads/sites/8/2023/08/NRMCB_2021.pdf22 Sep 2021: The human genome contains 5,000 integral mem-brane proteins, whose 20,000 TMDs differ widely in sequence, biophysical properties, location or locations within the protein and topology (Fig. ... The TMD- binding site spans the GET3 dimer and is a deep, 35-
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