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30 Dec 2002: ParM subunits are assem-bled head to tail into a protolament. Each successive subunit is rotatedby 166 and translated by 24.5 AÊ to create a helical lament. ... J. Appl. Crystallogr., 24,946950. Laskowski,R.A., MacArthur,M.W., Moss,D.S. and Thornton,J.M.

30 Jul 2002: age but lost its platform subdomain. The high degree of observation that there is a pool of epsin located at theGolgi [24]. ... 1996). Methods used in271, 12111–12116.the structure determination of bovine mitochondrial F1 ATPase.24.

20 Apr 2002: diC7PC (8 mM) no significant activation observed 0.85( 0.06 24.7( 2.9(1.7( 0.2). ... 132) PI-PLCδ1 and at 24-45 C for full-length PI-PLCδ1 (Figure 7A,B).

18 Apr 2002: B12. 24. 0.2 M Lithium Acetate, 20% PEG 3350. C1. 25. ... F12. 24. 0.2 M Ammonium Acetate, 0.01 M Ca Chloride, 0.05 M Na Cacodylate pH 6.5, 10% PEG 4000.

20 Apr 2002: PLC Activity in Mixed Micelles—The assay of hydrolysis of PIP2 andPI was based on methods described previously (19, 21, 24, 25). ... Thecalcium dependence of PIP2, PIP, and PI hydrolysis was analyzed usinga range of calcium buffers as described

20 Apr 2002: Deletion variants of PLC-N1 and PLC-Qlacking this second lobe are inactive, probably dueto destabilising the fold of the enzyme [23,24].Although sequence similarity throughout the EF-. ... Bio-phys. Res. Commun. 211 (1995) 364369. [24] Y. Emori, Y. Homma,

20 Apr 2002: 24). Thetopology of the domain is identical to that of phospholipase Cd1and the recently reported calcium-independent C2 domainfrom protein kinase Cd (23), whereas it is a circular permuta-tion of ... 24. Sutton, R. B., and Sprang, S. R. (1998) Structure

20 Apr 2002: baculovirus expression of Src family kinases were madeby subcloning the cDNAs (24) encoding activated Src (Y527F Src),activated Fyn (Y531F Fyn), and activated Lck (Y525F Lck) into thebaculovirus transfer vector ... Some of the additional sites couldbe

20 Apr 2002: Negatively charged regions are shown in red and positively chargedin blue (ranging from 18 kT/e to 24 kT/e). ... Crystallogr.heavy-atom parameter refinement for multiple isomorphous re- 24, 946–950.placement and multiwavelength anomalous diffraction

12 Oct 2002: Resolution (AÊ ) 2.0 2.0Observations/unique reections 83 922/24 307 84 280/24 356Completeness (last shell) % 93.6 (74.1) 93.7 (74.1)Rmerge. ... The supernatant was collected anddialysed against 50 mM TrisHCl pH 8.0 and 1.5 M urea for 16 h, andthen