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  2. Thesis Chapter 0

    https://www2.mrc-lmb.cam.ac.uk/groups/wschafer/Hardaker.pdf
    10 Jun 2008: UNIVERSITY OF CALIFORNIA, SAN DIEGO. Neural and molecular mechanisms. underlying behavioral state modulation in C. elegans. A thesis submitted in partial satisfaction of the. requirements for the degree Master of Science. in. Biology. by. Laura Anne
  3. ligand-fitting-tutorial.dvi

    https://www2.mrc-lmb.cam.ac.uk/personal/pemsley/coot/web/tutorial/ligand-fitting-tutorial.pdf
    24 Oct 2017: Tutorial: Ligand Fitting with Coot. CSHL 2014. October 24, 2017. 1 Introduction.
  4. Clay_thesis

    https://www2.mrc-lmb.cam.ac.uk/groups/wschafer/AnthonyKempf.pdf
    4 Oct 2005: the. 24. absence of fluorescence in the ventral cord and vulval area.
  5. cdf672 6935..6943

    https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/ParM%20EMBO%20F-actin%202002.pdf
    30 Dec 2002: ParM subunits are assem-bled head to tail into a protolament. Each successive subunit is rotatedby 166 and translated by 24.5 AÊ to create a helical lament. ... J. Appl. Crystallogr., 24,946950. Laskowski,R.A., MacArthur,M.W., Moss,D.S. and Thornton,J.M.
  6. pnas201206839 17424..17429

    https://www2.mrc-lmb.cam.ac.uk/groups/rlw/download/publications/23045692.pdf
    21 Nov 2012: Fig. 2A). As a positive controlfor cytokinesis failure, depletion of hIST1 resulted in a 14-foldaccumulation of multinucleated cells (24, 27). ... Mol Biol Cell 21(19):3293–3303. 24. Agromayor M, et al. (2009) Essential role of hIST1 in cytokinesis.
  7. N402_18f 313..320

    https://www2.mrc-lmb.cam.ac.uk/groups/rlw/download/publications/10580505.pdf
    11 Nov 1999: response to oxidative stress. Mol. Carcin. 24, 1524 (1999). 14. Yin, Y. ... UK, 1992). 24. CCP4. Collaborative Computing Project 4: A suite of programs for protein crystallography.
  8. Coot: Model-building tools for Molecular Graphics

    https://www2.mrc-lmb.cam.ac.uk/personal/pemsley/coot/files/presentations/coot-cshl-2018.pdf
    24 Oct 2018: Slide 19. Slide 20. Slide 21. Slide 22. Slide 23. Slide 24.
  9. Identifying proteins bound to native mitotic ESC chromosomes reveals…

    https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/Djeghloul%20Nat%20Camms%202020.pdf
    11 Aug 2020: 23.4 4.1, 43.6 6.9 µm2 respectively) or Eed/ (24.3 4.5,42.2 4.7 µm2, respectively) ESCs are significantly larger thanequivalent chromosomes isolated from WT ... 24 %. 70 %. async m-arrested. coun. t. PI intensity. Cardiomyocytes.
  10. Phosphoinositide-specific Phospholipase C: Structural Basis for…

    https://www2.mrc-lmb.cam.ac.uk/groups/rlw/download/publications/10024492.pdf
    11 Jun 1997: Based on sequence similarity and structural studies,modular PH, EF-hand and C2 domains have beendescribed in many proteins.22-24 PLCδ1 PH domainshares a high degree of structural similarity with ... 24. Kawasaki H, Kretsinger R (1994) Calcium-binding
  11. npgrj_nchembio_117 691..699

    https://www2.mrc-lmb.cam.ac.uk/groups/rlw/download/publications/18849971.pdf
    16 Oct 2008: 24),and an imidazoquinoline (5) inhibits the serine-threonine kinasePDK1 (ref. 25). The structural basis for these interactions isnot known. ... e) Cell cycle analysis by flow cytometry following treatment withPP121 or PI-103 (2.5 mM) or with vehicle (0.1
  12. PII: S0969-2126(02)00801-8

    https://www2.mrc-lmb.cam.ac.uk/groups/hmm/publica/Kent_McMahonOwen2002.pdf
    30 Jul 2002: age but lost its platform subdomain. The high degree of observation that there is a pool of epsin located at theGolgi [24]. ... 1996). Methods used in271, 12111–12116.the structure determination of bovine mitochondrial F1 ATPase.24.
  13. Structural Insights into Endosomal Sorting Complex Required…

    https://www2.mrc-lmb.cam.ac.uk/groups/rlw/download/publications/15044434.pdf
    24 Sep 2004: Received for publication, January 5, 2004, and in revised form, March 22, 2004Published, JBC Papers in Press, March 24, 2004, DOI 10.1074/jbc.M400023200. ... 2002) Biochim. Biophys. Acta 1579, 219 –224. 24. Bishop, N., Horman, A., and Woodman, P.
  14. Model-Building with Coot Adding N-linked Glycosylation& Demo Paul …

    https://www2.mrc-lmb.cam.ac.uk/personal/pemsley/coot/files/coot-may-2017-aca-new-orleans.pdf
    29 May 2017: 24/40. Refinement Stabilizers. Using Coot's Real Space Refinement – (in default mode). – ... Slide 20. Slide 21. Slide 22. Slide 23. Slide 24. Slide 25.
  15. doi:10.1016/j.molcel.2004.08.030

    https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/Haering%20Mol%20Cell%202004.pdf
    20 Sep 2004: Molecular Cell, Vol. 15, 951–964, September 24, 2004, Copyright 2004 by Cell Press.
  16. Membrane Localization is Critical for Activation of the PICK1 BAR…

    https://www2.mrc-lmb.cam.ac.uk/groups/hmm/publica/MadsenGetherTraffic2008.pdf
    18 Oct 2008: C-terminal 24 residues downstream from the putative. internalization motif in the DAT C-terminus to Tac. ... Of interest, a recent study has suggested a lipid-binding. capacity also for the PDZ domain (24).
  17. pnas201210899 16522..16527

    https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/PNAS-2012-Aylett-16522-7.pdf
    12 Oct 2012: Cocrystals were obtained with Bt TubR and a 24-bpdouble-stranded oligonucleotide (tubC24). ... Each point isplotted over the 12th bp of the 24-bp hairpin, with the sequence shown be-low.
  18. 15211199233993 1..45

    https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/MreBHussain2018.pdf
    15 Mar 2018: For correspondence:. egarner@g.harvard.edu. †These authors contributed. equally to this work. Competing interests: The. authors declare that no. competing interests exist. Funding: See page 32. Received: 03 October 2017. Accepted: 21 February
  19. Reconstitution of a prokaryotic minus end-trackingsystem using TubRC…

    https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/PNAS-2015-Fink-E1845-50.pdf
    27 Apr 2015: ProcNatl Acad Sci USA 80(16):4922–4925. 24. Howard J, Hudspeth AJ, Vale RD (1989) Movement of microtubules by single kinesinmolecules. ... J Bacteriol 196(24):4304–4314. 30. Westermann S, et al. (2006) The Dam1 kinetochore ring complex moves
  20. Mediation, Modulation, and Consequences of Membrane-Cytoskeleton…

    https://www2.mrc-lmb.cam.ac.uk/groups/hmm/publica/DohertyMcMahonAnnRevBiophys08.pdf
    22 May 2008: sona. l use. onl. y. ANRV343-BB37-04 ARI 24 April 2008 15:9. ... l use. onl. y. ANRV343-BB37-04 ARI 24 April 2008 15:9. Endocyticevents.
  21. Relocalization of STIM1 for Activation of Store-operated Ca2!Entry Is …

    https://www2.mrc-lmb.cam.ac.uk/groups/hegde/download/47_Ong_HL_JBC_2007.pdf
    24 Sep 2015: at MRC Lab of M. olecular Biology on September 24, 2015. ... evaluate apoptosis, using the method described previously (24).TIRF Imaging—HSG cells were seeded and cultured over-.
  22. 1 Supplementary data to accompany paper: Mechanism of endophilin ...

    https://www2.mrc-lmb.cam.ac.uk/groups/hmm/publica/GallopEndophilinEMBO/EndoSupMethods.pdf
    8 Jun 2006: Long. sample columns were used. Scans (averaging 10 readings) were taken at 280 nm at 24 hr.
  23. Catalytic Domain of Phosphoinositide-specificPhospholipase C…

    https://www2.mrc-lmb.cam.ac.uk/groups/rlw/download/publications/9565585.pdf
    20 Apr 2002: PLC Activity in Mixed Micelles—The assay of hydrolysis of PIP2 andPI was based on methods described previously (19, 21, 24, 25). ... Thecalcium dependence of PIP2, PIP, and PI hydrolysis was analyzed usinga range of calcium buffers as described
  24. JFCregg_OGS_finaledit

    https://www2.mrc-lmb.cam.ac.uk/groups/wschafer/Cregg_thesis.pdf
    14 Dec 2010: Egg-laying. 17. Neurophysiology in C. elegans. 24. Electrophysiology. 25. vi. Calcium Imaging. ... receptors. The worms will eventually (in 12 – 24 hrs) adapt to high levels of.
  25. Protein-driven membrane stresses in fusion and fission

    https://www2.mrc-lmb.cam.ac.uk/groups/hmm/publica/Papers/KozlovCurvature2010.pdf
    21 Feb 2011: Frequent observations of a complex network ofconnected tubules with an extensive surface area implythat considerable EHD2-driven fusion occurs betweentubulated liposomes [24]. ... 2009) The BAR domain superfamily: membrane-molding macromolecules. Cell 137
  26. Structural analysis of the ParR/parC plasmidpartition complex Jakob…

    https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/MollerJensen_ParR_EMMBO_2007.pdf
    4 Oct 2007: Diffraction data NATI, 30.7–2.8 Å, all dataR-factor, R-freea 0.24 (0.38), 0.28 (0.45)B-factorsb 54.9 Å2, 1.74 Å2. ... EMBO J 24: 270–282. Lim GE, Derman AI, Pogliano J (2005) Bacterial DNA segregation bydynamic SopA polymers.
  27. Doc2b Is a High-Affinity Ca2+ Sensor forSpontaneous Neurotransmitter…

    https://www2.mrc-lmb.cam.ac.uk/groups/hmm/publica/Papers/GroffenScience2010.pdf
    30 Apr 2010: S1) (24). Doc2b/ mice did not expressthe remaining exons and lacked Doc2b immuno-reactivity. ... Neurosci. 24, 1226 (2004).16. A. Maximov, O. H. Shin, X. Liu, T.
  28. PII: S1388-1981(99)00150-X

    https://www2.mrc-lmb.cam.ac.uk/groups/rlw/download/publications/10570253.pdf
    20 Apr 2002: Deletion variants of PLC-N1 and PLC-Qlacking this second lobe are inactive, probably dueto destabilising the fold of the enzyme [23,24].Although sequence similarity throughout the EF-. ... Bio-phys. Res. Commun. 211 (1995) 364369. [24] Y. Emori, Y. Homma,
  29. 7400749.qxd

    https://www2.mrc-lmb.cam.ac.uk/groups/hegde/download/41_Ashok_A_EMBORep_2006.pdf
    19 Jun 2006: Keywords: prions; retrovirus budding; exosome; endosomal transport;lysosome; multivesicular bodySubmitted 24 May 2006; accepted 31 May 2006.
  30. Pi-PEG Screen 1.5 disclaimer

    https://www2.mrc-lmb.cam.ac.uk/groups/JYL/WWWrobots/LMB%20plates/LMB19b-Pi-PEG_Screen_1.5_disclaimer.pdf
    27 Sep 2022: 6.45 % 1-23 PEG 600 25.65 % BICINE 75 mM 8.4 PEG 2000 MME 6.45 % 1-24 PEG 1000 22.5 % BICINE 75 mM 8.8 PEG 3000 ... 8 PEG 3000 12.9 % 1-35 PEG 200 24.15 % BICINE 75 mM 8.4 PEG 4000 10.65 % 1-36 PEG 300 21.45 % BICINE 75 mM 8.8
  31. CAN-11-2008 3642..3651

    https://www2.mrc-lmb.cam.ac.uk/groups/hegde/download/79_Basseville_A_CanRes_2012.pdf
    24 Sep 2015: Cancer Res; 72(14) July 15, 2012 Cancer Research3644. on September 24, 2015. ... Cancer Res; 72(14) July 15, 2012 Cancer Research3648. on September 24, 2015.
  32. Bacterial actin: architecture of the ParMRC plasmidDNA partitioning…

    https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/ParMRC%20Salje%202008%20EMBO.pdf
    4 Aug 2008: filament. The EMBO Journal (2008) 27, 2230–2238. doi:10.1038/. emboj.2008.152; Published online 24 July 2008.
  33. Coot Updates Paul EmsleySept 2016 Coot 0.8.4 Released● Patterson ...

    https://www2.mrc-lmb.cam.ac.uk/coot/ligands/2016/emsley-ccp4-ligand-meeting-sept-2016-v5.pdf
    28 Sep 2016: 24 (for 8HX). – now generate the same results. – call the tautomer enumeration with a molecule that does not contain hydrogen atoms. ... Slide 24. Slide 25. Slide 26. Slide 27. Slide 28. Slide 29.
  34. A Polymerization-Associated Structural Switch in FtsZ That Enables…

    https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/mBio-2017-Wagstaff-.pdf
    3 May 2017: this state (21, 23, 24). ... One of the conse-quences of such a switch, namely, permitting cooperative assembly of a single-strandedfilament, have been discussed previously (24, 27, 29, 39); however, such a switchconfers additional surprising
  35. The EMBO Journal Vol.17 No.18 pp.5273–5285, 1998 Crystal structure ...

    https://www2.mrc-lmb.cam.ac.uk/groups/hmm/publica/Owen_McMahon1998.pdf
    9 Sep 1998: 10.2 (24.1) 9.0 (17.9)Riso (%). c 32.5Phasing power 1.29 (0.77)Anomalous phasing power 1.33 (0.57)Resolution in outer shell (Å) ... 2.20 3.24–3.0Rfactor 0.190Rfree (10% of data) 0.258.
  36. Biochem. J. (2011) 440, 185–193 (Printed in Great Britain) ...

    https://www2.mrc-lmb.cam.ac.uk/groups/hmm/publica/Papers/ChernomordikBJ2011.pdf
    15 Nov 2011: B) Expression of the PLCδ1PH–GFP construct in Sf9Op1D cells inhibitedcell fusion that was triggered 24 h post-transfection. ... Rev. Mol. Cell Biol. 5, 133–147. 24 Mettlen, M., Pucadyil, T., Ramachandran, R.
  37. doi:10.1016/j.neuron.2005.05.004

    https://www2.mrc-lmb.cam.ac.uk/groups/hmm/publica/Jockusch_Neuron2005.pdf
    17 Jun 2005: 223 40 pA; 5M GDP-β-s and 0.5 mM ATP, 83 9 fF and 173 24 pA. ... 2004). Monitoring clathrin-mediated endocytosis during synaptic activity. WmJ. Neurosci. 24, 2004–2012.nNeves, G., and Lagnado, L.
  38. A positively charged channel within the Smc1/Smc3 hinge required for…

    https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/embosmcjingekurze2010.pdf
    1 Dec 2010: Smc1DDSmc3AAA. Smc1Smc3AAA. Kd = 22 nM Kd = 29 nM Kd = 24 nM. ... The Kd for Smc1DD and Smc3AAA hingedomain association is 29 nM (middle panel) and 24 nM for Smc1 wild-type and Smc3AAA (right panel).
  39. Molecular Biology of the CellVol. 19, 5093–5103, December 2008 ...

    https://www2.mrc-lmb.cam.ac.uk/groups/hmm/publica/Papers/LynchMBC5093.pdf
    24 Jan 2009: Mean F values (SD)for wild-type (3.9 24.9) and Syt-1RK (2.8 21.6) did not differ, whereas those for Syt-14W (15.1 16.8) differed ... vesicles. During a 5-min incubation in de-polarizing medium, an average of 22.6 3.5 vesicles inwild-type cells and 24.6
  40. pq240013132p

    https://www2.mrc-lmb.cam.ac.uk/groups/hegde/download/16_Wilhelm_JE_PNAS_2000.pdf
    10 Nov 2000: 24. prolactin coding region of Prl (excised with NcoI and BlpI) witha PCR product encoding full-length b-lactamase (b-lac). ... 24). They were used within 5 days of harvest.Oocytes were staged according to published criteria, and tran-scripts were
  41. Roles of Amphipathic Helices and the Bin/Amphiphysin/Rvs(BAR) Domain…

    https://www2.mrc-lmb.cam.ac.uk/groups/hmm/publica/Papers/Jao_JBC2010.pdf
    4 Oct 2010: Distances 20Å were investigated using four-pulse DEER (24–27), whereasshorter distances were investigated using continuous wave EPR(28, 29) (see “Experimental Procedures”).The DEER measurements are illustrated with the example ... Mutant
  42. x15959u064

    https://www2.mrc-lmb.cam.ac.uk/groups/hmm/publica/Reim_Brose2001.pdf
    17 Mar 2003: Cell, Vol. 104, 71–81, January 12, 2001, Copyright 2001 by Cell Press. Complexins Regulate a Late Step in Ca21-DependentNeurotransmitter Release. cellular Ca21 concentration. Usually, only a fraction oftethered vesicles in any given synapse is
  43. PII: S1097-2765(02)00515-4

    https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/SMC%20cohesin%20paper%202002.pdf
    19 Apr 2002: Molecular Cell, Vol. 9, 773–788, April, 2002, Copyright  2002 by Cell Press. Molecular Architecture of SMC Proteinsand the Yeast Cohesin Complex. other eukaryotes so far studied and several have alsobeen implicated in sister chromatid cohesion
  44. bc219914979p

    https://www2.mrc-lmb.cam.ac.uk/groups/rlw/download/publications/10329700.pdf
    20 Apr 2002: 24). Thetopology of the domain is identical to that of phospholipase Cd1and the recently reported calcium-independent C2 domainfrom protein kinase Cd (23), whereas it is a circular permuta-tion of ... 24. Sutton, R. B., and Sprang, S. R. (1998) Structure
  45. Transmembrane Domain Modulates Sorting of MembraneProteins in…

    https://www2.mrc-lmb.cam.ac.uk/groups/hegde/download/29_Karsten_V_JBC_2004.pdf
    2 Jun 2004: glass coverslips in 24-well plates were infected with trans-fected parasites and were processed for IF 20 –24 h post-transfection asdescribed (32). ... 23 Golgi Type I TM 90BAPVSVG 24 Golgi Type I TM 905-poly(L)ext 26 Golgi ND ND.
  46. Coot: Model-building tools for Molecular Graphics

    https://www2.mrc-lmb.cam.ac.uk/personal/pemsley/coot/files/presentations/bgu/coot-2018-bgu.pdf
    22 Feb 2018: Slide 20. Slide 21. Slide 22. Slide 23. Slide 24. Slide 25.
  47. Modeling membrane shaping by proteins: Focus on EHD2 and N-BAR domains

    https://www2.mrc-lmb.cam.ac.uk/groups/hmm/publica/Papers/KozlovEHDs.pdf
    7 Apr 2010: 18–20]), the BAR domain con-taining proteins [14,21–24], EHD2 [25], the complexes of clathrinwith adaptor and accessory proteins (see for reviews e.g.
  48. Membrane transport between compartments in eukary-otic cells requires …

    https://www2.mrc-lmb.cam.ac.uk/groups/hmm/publica/Dynaminreview2004.pdf
    2 Feb 2004: Membrane transport between compartments in eukary-otic cells requires proteins that allow the budding andSCISSION of nascent cargo vesicles from one compartmentand their targeting and fusion with another. Just asSNARES are proposed to be essential
  49. Systematic and Statistical Analysis to Aid Improvement and Validation …

    https://www2.mrc-lmb.cam.ac.uk/personal/pemsley/coot/files/2014_Oct_LIGANDS.pdf
    10 Oct 2014: Completed in 24.8393 seconds. CalculaNng bond class staNsNcs. WriNng bond class staNsNcs file: bond_class_stats.txt Completed in 9.53772 seconds.
  50. Wild card macros

    https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/sciencegayathri2012/Gayathri.SM.pdf
    23 Oct 2012: microscopy experiments within 24 hours. The formation of the RC complex was confirmed by gel shift assays (data. ... Residues 1-160 and 305-320 of domain I were used for rigid-body fitting using Chimera (24).
  51. Tumour prevention by a single antibody domaintargeting the…

    https://www2.mrc-lmb.cam.ac.uk/groups/rlw/download/publications/17568777.pdf
    22 Jul 2007: Fisher et al, 2001).Received: 24 January 2007; accepted: 9 May 2007; published online:14 June 2007. ... methane) and set in 24-well Cryschem plates (Hampton Research),with 400 ml of reservoir solution.

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