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Four-stranded mini microtubules formed by Prosthecobacter BtubAB show …
https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/PNAS-2017-Deng-E5950-8.pdf29 Dec 2017: For BtubAB filament reconstruction, 1,394 movies were usedfrom two separate 24-h microscope sessions. ... S1). For BtubABC filament reconstruction, 6,105 movies were used from fourseparate 24-h microscope sessions. -
Clay_thesis
https://www2.mrc-lmb.cam.ac.uk/groups/wschafer/AnthonyKempf.pdf4 Oct 2005: the. 24. absence of fluorescence in the ventral cord and vulval area. -
doi:10.1016/j.coph.2005.03.002
https://www2.mrc-lmb.cam.ac.uk/groups/rlw/download/publications/15963759.pdf12 Nov 2006: Mol Cell 2005, 18:13-24. A convincing and important description of the first selective S473-PKBphosphatase. ... 24. Welch HC, Coadwell WJ, Stephens LR, Hawkins PT:Phosphoinositide 3-kinase-dependent activation of Rac.FEBS Lett 2003, 546:93-97. -
DOI: 10.1126/science.1165322 , 1710 (2008); 322Science et al.Rachel…
https://www2.mrc-lmb.cam.ac.uk/groups/rlw/download/publications/19008417.pdf18 Dec 2008: 80, 7625 (2006).24. We thank O. Perisic and I. Duggin for advice and helpful. -
The Structure of a Tunicate C-type Lectin from Polyandrocarpa…
https://www2.mrc-lmb.cam.ac.uk/groups/rlw/download/publications/10398588.pdf7 Jul 1999: Joint CCP4 and ESF-EACBM Newsletter for Protein Crystallography, 33, 22-24. Gabius, H. ... Biophys.Biomol. Struct. 24, 551-577. Sambrook, J., Fritsch, E. F. & Maniatis, T. -
Crystal Structure of a Calcium-Phospholipid Binding Domainfrom…
https://www2.mrc-lmb.cam.ac.uk/groups/rlw/download/publications/9430701.pdf20 Apr 2002: Crystal Structure of a Calcium-Phospholipid Binding Domainfrom Cytosolic Phospholipase A2. (Received for publication, September 24, 1997). ... 1991) J. Appl. Crystallogr. 24, 946 –95072. Nicholls, A. (1992) GRASP: Graphical Representation and Analysis -
mk069902063p
https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/Fitting.MBC.pdf26 May 1999: J. Appl. Crystallogr. 24, 946 –950. Kull, F.J., Sablin, E.P., Lau, R., Fletterick, R.J., and Vale, R.D. -
Influence of lipid bilayer on the structure of the muscle-type…
https://www2.mrc-lmb.cam.ac.uk/groups/nu/pdf/pnasS24.pdf30 Apr 2024: in cell- derived membrane vesicles (24), or by in situ cryoelectron tomography (25). ... Electron Microscopy Data Bank https://www.ebi.ac.uk/emdb/EMD-18845. Deposited 6 November 2023. 24. N.Unwin, Influence of lipid bilayer on structure of acetylcholine -
JCSG+ Screen
https://www2.mrc-lmb.cam.ac.uk/screens2/pdf/LMB15.pdf2 Oct 2014: 0. 4. 8. 12. 16. 20. 24. Coun. ts. Detergents used for membrane protein crystallisation. ... 24. 0.1 M sodium chloride. 0.1 M magnesium chloride. 0.1 M CAPSO. -
Journal of Visualized Experiments www.jove.com Copyright © 2018…
https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/JOVE2018.pdf19 Apr 2018: JBS6 JenaBioScience CS-106L 24 Incomplete factorial based on AmS (pH 4.6-8.5). ... JBS7 JenaBioScience CS-107L 24 Incomplete factorial based on MPD (pH 4.6-8.5). -
doi:10.1016/j.cub.2004.09.077
https://www2.mrc-lmb.cam.ac.uk/groups/hmm/publica/Snx1PeteCullen2004/Snx1PeteCullen2004.pdf23 Nov 2004: Curr. Opin.Cell Biol. 14, 454–462. 24. Seaman, M.N.J., Marcusson, E.G., Cereghino, J.L., and Emr,S.D. -
PII: S0014-5793(01)02216-5
https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/MinD%20FEBS.pdf5 Mar 2001: 1990) Annu.Rev. Genet. 24, 249274. [3] Lutkenhaus, J. and Addinall, S.G. ... Appl. Crystallogr. 24, 946950.[34] Merritt, E.A. and Bacon, D.J. (1997) in : Macromolecular Crys-. -
�������� ��� � ���� �� �� ���� ��� ������ ...
https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/FtsA%20EMBO%20.pdf4 Oct 2000: E#N# 04412 (B<-! A # # " 3 16G7H#. # >$$I# ># 3 J#3# 04492 ' ( ' ) % % # ) &. 2'99G997#. $# B # O (# 0442 ( (- $ $ 6H $ # # / 0/ 24 64HG647#. (#-# -
1 CURRICULUM VITAE Venki Ramakrishnan Nationality U.S. (since 1985)…
https://www2.mrc-lmb.cam.ac.uk/groups/ribo/download/ramakrishnan_cv_4.pdf4 Jan 2024: Nat Struct Mol Biol 24, 866-869. N. R. James, A. Brown, Y. ... 123, 24-34. Gerchman, S.E. and Ramakrishnan, V. (1987). Chromatin higher-order structure studied by neutron scattering and scanning transmission electron microscopy. -
KWRM_A_992668_O
https://www2.mrc-lmb.cam.ac.uk/groups/wschafer/2015-3.pdf20 Oct 2015: Submitted: 11/06/2014. Revised: 11/21/2014. Accepted: 11/24/2014. http://dx.doi.org/10.4161/21624054.2014.992668This is an Open Access article distributed under theterms of the ... Molecular anatomy of a trafficking organelle.Cell 2006; 127:831-46; -
Nature © Macmillan Publishers Ltd 1998 8 30. Fan, ...
https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/FtsZ.Nature.pdf9 Jan 1998: Biochem. 24, 255–302 (1995). 22. Leslie, A. G. W. Recent changes to the MOSFLM package for processing film and image plate data (SERCLaboratory, Daresbury, Warrington, UK, 1991). ... 23. The CCP4 suite: Programs for protein crystallography. Acta -
doi:10.1016/j.pbiomolbio.2004.07.009
https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/stock2005robot.pdf19 Jan 2005: Quik phosphate grid screen 24 Hampton Research. NaCl grid screen 24 Hampton Research. ... The platform also accommodates most commercially availablecrystallisation plates (24-well Linbro (Hampton Research, #HR3-110), 24-well Cryschem(Hampton Research, -
PII: 0304-3991(84)90197-9
https://www2.mrc-lmb.cam.ac.uk/groups/nu/pdf/ultram84.pdf26 Oct 2008: The capacity of the Dewar is sufficient to give a running time of about 24 h under these conditions. -
Cryo-EM reconstruction of AlfA from Bacillus subtilis reveals the…
https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/AlfA2018.pdf20 Mar 2018: The subunits are staggered in a left-handed, double-heli-cal, parallel (polar) filament, with a rise of 24.9 Å and a 156.5 twist. ... Science 338:1334–1337. 24. Schumacher MA, et al. (2007) Segrosome structure revealed by a complex of ParRwith -
A Large Particle Associated with the Perimeter of the ...
https://www2.mrc-lmb.cam.ac.uk/groups/nu/pdf/jcb82.pdf5 Jul 2010: Best preserved porecomplexes show evidence of 16- and 24-fold harmonics inaddition to the basic eightfold harmonic (Fig. ... J. CellBiol. 52:338-354. 24. Paine, P. L. , L, C. Moore, and S. -
53091 361..366
https://www2.mrc-lmb.cam.ac.uk/groups/hmm/publica/McMahonE.pdf3 Dec 2004: 24). Thus our experiments point to a pioneering role forepsin in vesicle budding as it provides both a driving force and a linkbetween membrane invagination, clathrin polymerization and AP2complex recruitment. ... beta 2-adaptin appendage domain. EMBO J. -
nature structural biology . volume 5 number 6 . june 1998
https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/nsb1998.pdf14 Jan 2002: 24.Chook, Y.M., Gray, J.V., Ke, H. & Lipscomp, W.N. The monofunctional chorismate mutase from Bacillus subtilis: structure determination of chorismate mutase and its complexes with a transition state ... J. Appl. Cryst. 24, 946-950 (1991). 39.Merrit, E.A. -
Structure of bacterial tubulin BtubA�B:Evidence for horizontal gene…
https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/btubab%202005.pdf21 Jun 2005: BtubAB form the same het-erodimer as tubulin (24, 25). The protofilament axis isvertical. ... 1993) Micron 24, 465– 471. 10. Petroni, G., Spring, S., Schleifer, K.-H., Verni, F. & -
Activation of Xer-recombination at dif: structural basis of the…
https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/srep33357.pdf30 Sep 2016: identification of a potential N-terminal targeting domain. J Bacteriol 180, 1296–1304 (1998).24. ... EMBO J 24,. 3770–3780, doi: 10.1038/sj.emboj.7600835 (2005).29. Levy, O. et al. -
Organization of spines on the dendritesof Purkinje cellsJohn O’Brien…
https://www2.mrc-lmb.cam.ac.uk/groups/nu/pdf/pnas06.pdf25 Jul 2006: 2004) Nat. Rev. 5, 24 –34.4. Matus, A. (2000) Science 290, 754 –758.5. ... T., Wong, R. O. L. & Lichtman, J. W. (2000) Neuron 27, 219 –225.24. -
se289900215p
https://www2.mrc-lmb.cam.ac.uk/groups/hmm/publica/strucrev.pdf6 Jul 1999: Adaptors EM, rotary shadow and deep-etch; APcomplexes (13). ;25 (2D). Crystallography; AP2 m2 residues 158 to435 (24). ... 2.7 Phe174, Arg423 (24) YxxØ endocytosis signals. Crystallography; AP2 COOH-terminala-adaptin appendage domain residues701 to 938 -
Structure and superorganization of acetylcholinereceptor–rapsyn…
https://www2.mrc-lmb.cam.ac.uk/groups/nu/pdf/pnas13.pdf3 Jul 2013: 20%of the receptors showed restricted diffusion in small domains of50 nm, whereas the majority of the receptors were immobile (24).Confocal microscopy of overexpressed rapsyn in nonmuscle. ... The cross-linking of receptors by rapsyn,therefore, seems to -
1202.tif
https://www2.mrc-lmb.cam.ac.uk/groups/nu/pdf/jcb84.pdf16 Jun 2006: Science (Wash. DC). 208:1454-1457. 24. Ross, M. J., M. W. Klymkowsky, D. ... J. Ultrastruct. R e s 24:454-564. 27. Ziogsheim, H.-P., D.-C. Neugehauer, F. -
Crystal structure of the SOS cell division inhibitorSulA and ...
https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/SulA%20PNAS%202003.pdf13 Jun 2003: To whom correspondence should be addressed. E-mail: jyl@mrc-lmb.cam.ac.uk. www.pnas.orgcgidoi10.1073pnas.1330742100 PNAS June 24, 2003 vol. ... Lowe, J. (1999) Struct.Folding Des. 7, 1181–1187. 24. Van Duyne, G. -
doi:10.1016/j.jmb.2004.05.031
https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/zapa%202004.pdf16 Aug 2004: In vitro, FtsZ isextremely versatile, forming protofilaments, thickfilaments, sheets,21,22 helical tubes23,24 and asters.25. ... FEMS Microbiol. Rev. 24,531–548. 13. Rothfield, L., Justice, S. & Garcia-Lara, J.(1999). -
se060101051p
https://www2.mrc-lmb.cam.ac.uk/groups/hmm/publica/ford01.pdf3 Dec 2004: The COOH-termi-nal domains of these proteins contain clath-rin-binding motifs (3, 24 ), as well as Asp-Pro-Phe (DPF)-like a- and b-adaptin–. ... De Camilli, A. T.Brunger, J. Cell Biol. 149, 537 (2000). 24. -
se060101051p
https://www2.mrc-lmb.cam.ac.uk/groups/hmm/AP180/Ap180_st.pdf3 Dec 2004: The COOH-termi-nal domains of these proteins contain clath-rin-binding motifs (3, 24 ), as well as Asp-Pro-Phe (DPF)-like a- and b-adaptin–. ... De Camilli, A. T.Brunger, J. Cell Biol. 149, 537 (2000). 24. -
Bacterial actin MreB assembles in complex with cell shape protein RodZ
https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/embo2010RodZMreB.pdf17 Feb 2010: The crystals were cryoprotected with mother liquorcontaining 24.8% PEG400 and flash frozen in liquid nitrogen. ... The plate was incubated at 301Cand imaged after 24 h. DrodZ complementation assays and microscopy. -
PII: 0896-6273(95)90037-3
https://www2.mrc-lmb.cam.ac.uk/groups/nu/pdf/neuron95.pdf4 May 2004: Number of Receptors Phase Residual" Weight in Final. scFv35 Film number 4280 3560 23.5 0.18 Film number 4282 4360 24.2 0.23 Film number 4295 4820 16.1 ... Biochemistry 24, 1212- 1221. -
Tutorial 4: Statistical Theory;Why is the Gaussian Distribution so ...
https://www2.mrc-lmb.cam.ac.uk/groups/murshudov/content/courses/lmb_statistics_course_2014/tutorials/statistical_theory_why_is_the_gaussian_distribution_so_popular_answers.pdf25 Mar 2014: If X Bin(100, 0.4) then E(X) = 40 and V ar(X) = 24. ... 8. pnorm(35,40,sqrt(24)). Answer: 0.154 (3sf). Statistical Theory; Why is the Gaussian Distribution so popular? -
FigS1
https://www2.mrc-lmb.cam.ac.uk/groups/hmm/publica/SchmidBetaPaper/BetaHubSup.pdf7 Sep 2006: AP180(27), Eps15(6). Dynamin1,2,3 (89,30,24), Amph1(24). Eps15R(52), AP180(5), Dynamin1(1). Amph1(15), Eps15R(2). Amph2(40), Epsin1(1). -
Activation of GCN2 by the ribosomal P-stalk
https://www2.mrc-lmb.cam.ac.uk/groups/hegde/download/PNAS_2019.pdf7 Mar 2019: Yeast GCN2 forms aconstitutive dimer even in the absence of activation, principallythrough the CTD (24, 25). ... This lack of clarity was further con-founded by a more recent report that, unlike yeast, mouseGCN2 does not form a stable complex that -
Collaborative protein filaments
https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/ghosal_embo_2015.pdf4 Jan 2016: Mol. Microbiol 24: 905 – 915. van den Ent F, Izore T, Bharat TA, Johnson CM, Löwe J (2014) Bacterial actin. ... EMBO J 24: 270 – 282. Lesterlin C, Ball G, Schermelleh L, Sherratt DJ (2014) RecA bundles mediate. -
The MORPHEUS protein crystallization screen
https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/morpheus%202009.pdf12 Nov 2009: 1991). J. Appl. Cryst. 24, 409–411.Joerger, A. C., Ang, H. C. & ... m, n. Ran Ran GTPase 10.0 24.5 LMB, Danguole Ciziene Stewart et al. -
www.landesbioscience.com Autophagy 805 Autophagy 6:6, 805-807; August …
https://www2.mrc-lmb.cam.ac.uk/groups/rlw/download/publications/20574157.pdf2 Aug 2010: Submitted: 05/24/10. Revised: 06/03/10. Accepted: 06/03/10. Previously published online: www.landesbioscience.com/journals/autophagy/article/12577Correspondence to: Roger L. -
1256917combinedsupplement
https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/Gligoris-SM.pdf14 Nov 2014: Curr. Biol. 21, 12–24 (2011). Medline doi:10.1016/j.cub.2010.12.004. 13. E. Unal, J. ... Cell 103, 387–398 (2000). Medline doi:10.1016/S0092-8674(00)00131-8. 24. A. Losada, M. Hirano, T. -
Mechanism of ribosome stalling during translation of a poly(A) tail
https://www2.mrc-lmb.cam.ac.uk/groups/hegde/download/NSMB_2019.pdf25 Nov 2019: The stalled ribosome triggers the ‘no-go’ or ‘nonstop’ pathways of mRNA decay10,13,17,18, nascent protein degra-dation via ribosome-associated quality control12,19–23 and ribosome ... 24,25,30,31). A second mechanism involves detecting the -
se060101047p
https://www2.mrc-lmb.cam.ac.uk/groups/hmm/publica/Itoh%20et%20al.pdf23 Nov 2004: Quantitativerepresentations of co-sedimentation assay data of GST-ENTH domains from (D) epsin and (E)AP180 (24) with 0.5% of the indicated phospholipids in the liposomes. ... X-ray Crystallography and NMR (Yale Univ. Press,New Haven, CT, 1993). 24. -
cde235 2454..2461
https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/MinC%20EMBO%202001.pdf4 May 2001: SeMet-substituted crystals were grown in thesame manner as for the native protein but with 24% PEG 2000, 0.1 Msodium citrate pH 4.8, 3.6% ethanol for the crystallization ... J. Appl. Crystallogr., 24,946950. Laskowski,R.A., Macarthur,M.W., Moss,D.S. and -
st7a11.qxd
https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/MukB%20Structure%201999.pdf10 Sep 1999: 15, 430-434. 24. Akhmedov, A.T., Frei, C., Tsai-Pflugfelder, M., Kemper, B., Gasser,S.M. & ... J. Appl. Crystallogr.24, 946-950. 33. Barton, G.J. (1993). ALSCRIPT: a tool to format multiple sequencealignments. -
BAR Domains as Sensors ofMembrane Curvature: TheAmphiphysin BAR…
https://www2.mrc-lmb.cam.ac.uk/groups/hmm/publica/PeterBARdomains.pdf30 Jan 2004: recruit clathrin to membranes. Mammalian am-phiphysin2 has several clathrin-binding sites (24–26), and we found that the wild-type, but notmut1, efficiently recruited clathrin to liposomes(fig. ... It is interesting to note that thestructure of the -
Supplementary Figure 1Lysophosphatidic acid acyl transferase (LPAAT)…
https://www2.mrc-lmb.cam.ac.uk/groups/hmm/publica/GallopEndoSupFigs.pdf4 Jan 2006: 35. 24. 21. Bead. s pos. t clea. vage. Purif. ied cl. -
M410_8e 231..235
https://www2.mrc-lmb.cam.ac.uk/groups/hmm/publica/Dynamin.pdf6 Mar 2001: 24. Vallis, Y., Wigge, P., Marks, B., Evans, P. R. & McMahon, H. -
pnas201313978 4601..4610
https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/pnas2013duman.pdf23 Dec 2013: AnezrA-null mutant forms slightly longer cells (20%) comparedwith wild-type cells (23), but an ftsA null mutant has problemsdividing and forms long filamentous cells (24). -
A-nsmb855.indd
https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/nsmb%20FtsZ%202004.pdf19 Nov 2004: Biol. Chem. 276, 17307–17315 (2001). 24. Andreu, J.M., Oliva, M.A. & Monasterio, O. ... J. Appl. Crystallogr. 24, 946–950 (1991). 41. Miroux, B. & Walker, J.E.
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