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23 Oct 2012: The image pixel size at this. magnification was 1.64 Å/pixel. In total of 200 CCD images were collected. ... µl were added to the 200 µl reaction mixture before initiating polymerization with 2 mM (final concentration).

15 Mar 2012: ParB (200 RU) was immobilized on an SPR sensor chip and allowed to react with wild-type MipZ protein (0.5 µM) that had been preincubated with 2 mM ATP and ... Subsequently, the protein was applied to a Sephacryl S-200 gel filtration column (GE Healthcare

21 Nov 2012: ni. WT/. icW. T/i. 0. 100. 200. 300. 400. CD/n. ic. ... 100. 200. 300. ΔReq. (R. U). 0100200300400500600700800. SH2 deletion lipid kinase dead cancer-linkedmutations:.

28 Sep 2012: The Coot User ManualPaul Emsley. paul dot emsley at bioch dot ox dot ac dot uk. i. Table of Contents. 1 Introduction. 11.1 Citing Coot and Friends. 11.2 What is Coot? 11.3 What Coot is Not. 11.4 Hardware Requirements. 2. 1.4.1 Mouse. 21.5

26 Nov 2012: In some experiments of this sort, using a range 0 to 200 V, the potential was found to vary along the thread proportionately to the electron flux. ... manner. The corresponding interference patterns indicate a n ear-zero potential, a high positive

5 Dec 2012: Subsequently, Gb1His-g2 dimers were eluted with abuffer containing 20 mM tris-HCl (pH 8.0), 25 mM NaCl, 0.1% C12E10,200 mM imidazole, and 10 mM 2-ME. ... ME. Gb1His-g2(C68S) mutants were eluted with abuffer containing 20 mM tris-HCl (pH 8.0), 25 mM NaCl,

17 Jan 2012: For polarized uptake experiments, confluent monolayers of MEFs, grown on 12-mm, round, glass coverslips (Lomb Scientific, Australia) were wounded by scratching with a 200 μl pipette tip. ... An axiovert 200 m SP LSM 510 META confocal laser-scanning micro

12 Oct 2012: Superstructure of the centromeric complex ofTubZRC plasmid partitioning systemsChristopher H. S. Aylett and Jan Löwe1. Medical Research Council Laboratory of Molecular Biology, Division of Structural Studies, Cambridge CB2 0QH, United Kingdom.

12 Jan 2012: Example:occupancy group id 1 chain A # chain A belongs to occupancygroup 1occupancy group id 1 chain B residues from 200 to 500 # all residues between residues200 and 500 of chain ... 150 A atoms C # all atoms with atom namesstarting with C for all

27 Mar 2012: Crystalswere transferred into the same conditions supplemented with 25%(v/v) PEG 200 before flash freezing.