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10 Sep 1999: mukB V.cholmukB H.inflmukB Y.pestmukB E.coli. S6 H4 H5 H6. ... Protein was eluted with the same buffercontaining 400 mM imidazole. The protein was applied to a sephacrylS-200 column (Amersham-Pharmacia) equilibrated in 20 mM Tris,200 mM NaCl, 1 mM

9 Dec 1999: sym. pto. mat. ic. Age (days)200 400 600 800. c. b(A. ... 80. 60. 40. 20. 00 100 200 300 400. (A117V) H(STE).

18 Feb 1999: 110 120 130 140 150 160 170 180 190 200. 210 220 230 240 250 260 270 280 290 300.

30 Apr 1999: 2,000. 500. a. Time (min). Time (min). 200. µM GTP. GT. ... ferase-tagged amphiphysin-2 SH3 domain on glutathione–agarose beads at 4 C. After extensive washing of the matrix in buffer A (200 mM NaCl, 20 mM HEPES, pH 7.3,

20 Apr 1999: Twenty four micrographs out of 200 in the tilt range of 0–60 wereselected and scanned on a Zeiss SCAI scanner with a 28µm step size.Images were processed with the

11 Nov 1999: Ras binding domain Nα1 Nα2 Nα3 Nα4 Rβ1 Rβ2 Rα1. 150 160 170 180 190 200 210 220 230 240 250. ... contain one protein molecule in the asymmetric unit.Diffraction data were collected at ESRF beamlines ID2 and ID14-4 at 100K after freezingcrystals in

6 May 1999: Thus anunfolding of the hinge sub-domain may expose theTolA-binding site on D1 and release the g3pdomains like beads on a string, allowing them tospan a large distance (> 200 AÊ )