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6 Apr 2017: Crystals wereflash-frozen in liquid nitrogen for data collection using an additional 25% (vol/vol)PEG 200 as cryo-protectant. ... 200 mesh grids in a Vitrobot Mark IV (FEICompany) maintained at 10 C at 100% relative humidity.

4 Aug 2017: 1, A and B). Cross-linking reactionsof the active fractions revealed interacting part-ners of 200, 120, and 100 kD (Fig. ... The gradients were either 2 ml or 0.2 ml, on which either 200 µl or 20 µl of sample were layered.

19 Jan 2017: vector and a 3’ primer that anneals 200 bp downstream of the stop codon of the open reading frame. ... DTT). During the buffer change, 1:200 of SuperTEV (for His-tagged proteins) or 3C protease (for GST-tagged proteins) were added for overnight

3 May 2017: All images are at the same magnification (scale bar, 200 nm). ... However, these filaments are not the sameas the open-form filaments, with a much smaller interface buried surface area (BSA) of700 Å2, compared to 1,200 Å2 for 1FOf and PDB

22 Sep 2017: available: the IMPAX and ORYX systems from Douglas Instruments Ltd., UK, and theCyberlab 200, distributed by Gilson Inc., USA.