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11 Nov 2008: HeLa cells that had been serum-starved for 16 hours were stimulated with either EGF (20 ng/ml), TPA (200 ng/ml) or isoproterenol (10 µM) at 37ºC and the activation ... 60. 65. 70. 75. 200 300 400 500 600 700 800 900 1000 1100 12000.

6 Feb 2008: Depending on molecular weight of the protein and quantity of the protein a Sephadex 200 or 75 16/60 or 26/60 (Amersham Biosciences) was used. ... Proteins were transferred from polyacrylamide gels onto nitrocellulose (Protean) at a limiting current of

18 Dec 2008: on. Dec. embe. r 18. , 200. 8 w. ww. sci. ... embe. r 18. , 200. 8 w. ww. sci. ence. mag.

15 Dec 2008: Membranes were stained using 10 µg/mL FM-464 for ten minutes. 200 µL of cells were taken directly from.

16 Aug 2008: tion plates mixing 200 200 nl of protein and crystallization solution. ... KOPS1 DNA. Crystallization solution was 200 mM citric acid/NaOH pH 3.5.

16 Oct 2008: More than 200 analogs of these hits wereiteratively synthesized through diversification of the R1 and R2substituents (Supplementary Table 1). ... We profiled PP121 and PP487against over 200 protein kinases and found that they inhibit a patternof tyrosine

28 Nov 2008: Note the protein-. dependent presence of tubular structures. The scale bar represents 200 nm. ... beads (Amersham Biosciences) and gel filtration on a sephacryl S-200 column (Amersham) as previously described [1].

4 Mar 2008: coli FtsQ and L. monocytogenes FtsQ, in20 mM Tris, 1 mM EDTA, 1 mM NaAzide, 200 mM NaCl,pH 7.0 (TEN200) for Y.

30 May 2008: 0.5 M sucrose in PSB, 200 µL) by centrifugation at 100,000 rpm for 45 min at 4 C in a.

4 Aug 2008: Plasmid 43:200–204. ParMRC plasmid partitioningJ Salje and J Löwe. The EMBO Journal VOL 27 | NO 16 | 2008 & 2008 European Molecular Biology Organization2238.