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  2. hmm lab christmas party 031205 200

    https://www2.mrc-lmb.cam.ac.uk/groups/hmm/people/Christmas2005/Christmas2005-Pages/Image63.html
    12 Jun 2006:
  3. 120 L fermentor: Quick guide

    https://www2.mrc-lmb.cam.ac.uk/groups/JYL/WWWfermentor/runfermentor.html
    27 Feb 2006: Press "Activate". Switch on stirrer to dissolve powder (Control: Ag(itation) speed=100-200 rpm). ... Stirrer 100-200, oxygen enrichment on (sp=0), air 60-100 (watch out for too much foam); NOTE: DO should be OFF.
  4. doi:10.1016/j.jbbm.2005.12.008

    https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/rfcloning2006%20published.pdf
    19 Apr 2006: Miroux personal communication), 200 AM dNTP’s (Roche), 1!reaction buffer,2.5 units PfuTurbok (Stratagene) in a total volume of 50 Al. ... TCF1 200 3–6 2.0 4.6 4/4 OK Yes nd. TCF2 200 3–6 2.0 4.6 4/4 OK Yes nd.
  5. NPGRJ_NSMB_1158 1..8

    https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/nsmbFtsK2006.pdf
    12 Oct 2006: c) Superdex 200 gel filtration of E. coli FtsKg in the presence of DNA. ... Storage buffer was. 20 mM Tris-HCl, 200 mM NaCl, 1 mM EDTA and 1 mM sodium azide.
  6. Acetylation of MEK2 and I{kappa}B kinase (IKK) activation loop…

    https://www2.mrc-lmb.cam.ac.uk/groups/hmm/publica/YopJ.pdf
    28 Nov 2006: Acetyl Transferase Assays. GST-MEK2 (0.5 g) (purified by usingstandard protocols for GST-tagged protein purification) wasincubated with varying amounts (0 –200 ng) of (His)6-YopJ-wt ... Stimulation of MEK and Erk phosphorylation by various
  7. Supplementary Figure 1Lysophosphatidic acid acyl transferase (LPAAT)…

    https://www2.mrc-lmb.cam.ac.uk/groups/hmm/publica/GallopEndoSupFigs.pdf
    4 Jan 2006: fter. th. rom. bin cl. eava. ge o. f GST. ). 0.01. 0.1. 1. 10. 0 100 200 300 400. ln OD600. ... 1. 10. 0 100 200 300 400. ln OD600. pPLSC (E.coli LPAAT).
  8. 1202.tif

    https://www2.mrc-lmb.cam.ac.uk/groups/nu/pdf/jcb84.pdf
    16 Jun 2006: 5) , andthe i r w i dth rema i ned fa i r l y constant (800 - 1 , 200 A).
  9. doi:10.1016/S0076-6879(05)04052-8

    https://www2.mrc-lmb.cam.ac.uk/groups/hmm/publica/HigginsMcMahonMethods2005.pdf
    26 Jan 2006: A lossof 100–200 l is usually observed. Purification of Dynamin and Its Mutants from E. ... Scale bars are 200 nm in. length. [52] IN VITRO RECONSTITUTION OF ENDOCYTIC STAGES 607.
  10. doi:10.1016/j.molcel.2006.06.019

    https://www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/Ftsk%20mc%202006.pdf
    12 Aug 2006: agent (200 ml in 700 ml water) was added and Pi release monitored. ... tios of these proteins were mixed (in buffer A plus 200 mM NaCl;.
  11. © 2005 Nature Publishing Group Endophilin and CtBP/BARS are ...

    https://www2.mrc-lmb.cam.ac.uk/groups/hmm/publica/GallopEndopohilinNature2005.pdf
    4 Jan 2006: fatty acid-free BSA and 0.01% Triton X-100 with substrate concentrations (20–200 mM LPA) at 25 8C and 37 8C with 14C-labelled oleoyl-CoA (Amersham) at aspecific ... J. Cell Biol. 155, 193–-200 (2001). 11. Huttner, W. B. & Schmidt, A.

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