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27 Mar 2002: A3. 3. 50% (v/v) PEG-200, 0.1M Citrate pH 5.5 (final pH 6.9). ... E2. 2. 50% (v/v) PEG-200, 0.1M CHES pH 9.5 (final pH 9.6).

18 Apr 2002: H1. 37. 0.2 M K Chloride, 0.025 M Mg Sulfate, 0.05 M HEPES - Na pH 7.0, 20% PEG 200.

3 May 2002: J. Cell. Biol.155, 193–200. f Takei, K. et al. (1999) Functional partnership betweenamphiphysin and dynamin in clathrin-mediated endocytosis. ... J. Cell. Biol. 155,193–200. 20 Takei, K. et al. (1999) Functional partnershipbetween amphiphysin and

3 May 2002: J. Cell. Biol.155, 193–200. f Takei, K. et al. (1999) Functional partnership betweenamphiphysin and dynamin in clathrin-mediated endocytosis. ... J. Cell. Biol. 155,193–200. 20 Takei, K. et al. (1999) Functional partnershipbetween amphiphysin and

20 Apr 2002: 21 ions. Enzyme(200 ng) was added to the subphase, via an injection port in the Teflontrough, 5 min after the monolayer was spread to allow the surfacepressure to stabilize. ... Replacement of these residues byalanine resulted in a great reduction of the

20 Apr 2002: The cell pellet was resus-pended in 200 l of lysis buffer (1% Triton, 150 mM NaCl, 10 mM Tris,pH 7.4, 1 mM EDTA, 1 mM EGTA, 0.2 ... PLC2 was further purified on a heparin-Sepharose column (Am-ersham Biosciences) followed by gel filtration on a Superdex

19 Apr 2002: Insect cell extracts containing defined concentrations of Smc1 as the ligand (five dilutions, ranging from 20nM to 200 nM) were floated over the bound analytes, and association and dissociation kinetics were

20 Apr 2002: a The wortmannin crystal was soaked in 1 mM wortmannin for 50 min.b The LY294002 crystal was soaked in 1 mM LY294002 for 200 min.c The quercetin crystal had ... Agullo, G., Gamet-Payrastre, L., Manenti, S., Viala, C., Rémésy, C.,[pH 7.2], 200 mM

30 Jul 2002: 24% PEG4000, 100 mMTris (pH 8.0), and 200 mM MgCl2.X-ray diffraction data were collected at 100 K in-house on a rotat- References.