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wash brains with PBT (2 x 10 min). remove PBT as completely as possible and add 200 µl of Vectashield (or other) mounting medium we observed that subsequently transferring brains into ... a fresh 200 ul aliquot of Vectashield results in more homogenous

wash brains with PBT (2 x 10 min). remove PBT as completely as possible and add 200 µl of Vectashield (or other) mounting medium we observed that subsequently transferring brains into ... a fresh 200 ul aliquot of Vectashield results in more homogenous

Protocol for chemical labeling of mouse brain slices. Vibratome slices (200 µm).

Protocol for chemical labeling of mouse brain slices. Vibratome slices (200 µm).

Acros. AC37091. phenylacetic acid. pac. 103-82-2. 1:200 (HO). Sigma. W287806. 11-cis-vaccenyl acetate.

200 µm coronal vibratome brain slices of animals injected with AAV-myrSNAP were incubated with 1 µM BG-549 for 30 min, 1 h, 6 h or 12 h.

long incubation times ( 6 h) can result in increased background staining!// wash brains with PBT (2 x 10 min) remove PBT as completely as possible and add 200 µl of Vectashield ... or other) mounting medium //we observed that subsequently transferring

Protocol for chemical labeling of mouse brain slices===== ===Vibratome slices (200 µm)=== fix slices in 4% PFA (in 0.1 M PB) at room temperature for 20 min in glass-well

200 µm coronal vibratome brain slices of animals injected with AAV-myrSNAP were incubated with 1 µM BG-549 for 30 min, 1 h, 6 h or 12 h.