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  2. si:tagging:tagging2014flystain - Jefferis Lab DokuWiki

    flybrain.mrc-lmb.cam.ac.uk/dokuwiki/doku.php?id=si:tagging:tagging2014flystain
    wash brains with PBT (2 x 10 min). remove PBT as completely as possible and add 200 µl of Vectashield (or other) mounting medium we observed that subsequently transferring brains into ... a fresh 200 ul aliquot of Vectashield results in more homogenous
  3. si:kohl2014:tagging2014flystain - Jefferis Lab DokuWiki

    flybrain.mrc-lmb.cam.ac.uk/dokuwiki/doku.php?id=si:kohl2014:tagging2014flystain
    wash brains with PBT (2 x 10 min). remove PBT as completely as possible and add 200 µl of Vectashield (or other) mounting medium we observed that subsequently transferring brains into ... a fresh 200 ul aliquot of Vectashield results in more homogenous
  4. si:tagging:tagging2014mousestain - Jefferis Lab DokuWiki

    flybrain.mrc-lmb.cam.ac.uk/dokuwiki/doku.php?id=si:tagging:tagging2014mousestain
    Protocol for chemical labeling of mouse brain slices. Vibratome slices (200 µm).
  5. si:kohl2014:tagging2014mousestain - Jefferis Lab DokuWiki

    flybrain.mrc-lmb.cam.ac.uk/dokuwiki/doku.php?id=si:kohl2014:tagging2014mousestain
    Protocol for chemical labeling of mouse brain slices. Vibratome slices (200 µm).
  6. pub:neuroanatomy:frulhns:odorants - Jefferis Lab DokuWiki

    flybrain.mrc-lmb.cam.ac.uk/dokuwiki/doku.php?id=pub:neuroanatomy:frulhns:odorants
    Acros. AC37091. phenylacetic acid. pac. 103-82-2. 1:200 (HO). Sigma. W287806. 11-cis-vaccenyl acetate.
  7. flybrain.mrc-lmb.cam.ac.uk/dokuwiki/doku.php?do=export_raw&id=si:t…

    flybrain.mrc-lmb.cam.ac.uk/dokuwiki/doku.php?do=export_raw&id=si:tagging:tagging2014flystain
    long incubation times ( 6 h) can result in increased background staining!// wash brains with PBT (2 x 10 min) remove PBT as completely as possible and add 200 µl of Vectashield ... or other) mounting medium //we observed that subsequently transferring
  8. si:tagging:fiber_analysis_in_mouse_brain_slices - Jefferis Lab…

    flybrain.mrc-lmb.cam.ac.uk/dokuwiki/doku.php?id=si:tagging:fiber_analysis_in_mouse_brain_slices
    200 µm coronal vibratome brain slices of animals injected with AAV-myrSNAP were incubated with 1 µM BG-549 for 30 min, 1 h, 6 h or 12 h.
  9. flybrain.mrc-lmb.cam.ac.uk/dokuwiki/doku.php?do=export_raw&id=si:t…

    flybrain.mrc-lmb.cam.ac.uk/dokuwiki/doku.php?do=export_raw&id=si:tagging:tagging2014mousestain
    Protocol for chemical labeling of mouse brain slices===== ===Vibratome slices (200 µm)=== fix slices in 4% PFA (in 0.1 M PB) at room temperature for 20 min in glass-well
  10. flybrain.mrc-lmb.cam.ac.uk/dokuwiki/doku.php?do=export_raw&id=pub:…

    flybrain.mrc-lmb.cam.ac.uk/dokuwiki/doku.php?do=export_raw&id=pub:neuroanatomy:frulhns:odorants
    1| 1:100 (oil)| Acros| AC37091| |phenylacetic acid| pac| 103-82-2| 1:200 (HO)| Sigma| W287806| |11-cis-vaccenyl acetate| cVA| 6186-98-7| pure| Pherobank| n/a| |apple cider
  11. flybrain.mrc-lmb.cam.ac.uk/dokuwiki/doku.php?do=export_raw&id=si:t…

    flybrain.mrc-lmb.cam.ac.uk/dokuwiki/doku.php?do=export_raw&id=si:tagging:fiber_analysis_in_mouse_brain_slices
    200 µm coronal vibratome brain slices of animals injected with AAV-myrSNAP were incubated with 1 µM BG-549 for 30 min, 1 h, 6 h or 12 h.

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