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27 Jan 2018: Briefly, cells were washed three times in PBS and collected by spinning at 200 x g for 5 min. ... The resin was washed five times with 0.1% digitonin, 200 mM NaCl, 50 mM HEPES.

3 Dec 2004: AP2was dialyzed against 50 mM triethanolamine-KCl,pH 8.0, 200 mM NaCl, 0.2 mM EDTA, 0.1% b-mer-captoethanol, and 0.02% NaN3.

4 Aug 2017: 1, A and B). Cross-linking reactionsof the active fractions revealed interacting part-ners of 200, 120, and 100 kD (Fig. ... The gradients were either 2 ml or 0.2 ml, on which either 200 µl or 20 µl of sample were layered.

30 Sep 2016: Crystallization and data collection for XerDC-FtsKγ. Initial hits were identified using 200 nl protein drops, using commercial screens (Molecular Dimensions) mixed by Mosquito nano-litre robot (TTP Labtech).

17 Jul 2009: 3. Farsad K. et al. J. Cell Biol. 155, 193–200 (2001).4.

12 Aug 2006: agent (200 ml in 700 ml water) was added and Pi release monitored. ... tios of these proteins were mixed (in buffer A plus 200 mM NaCl;.

16 Jun 2006: 5) , andthe i r w i dth rema i ned fa i r l y constant (800 - 1 , 200 A).

19 Jan 2005: available: the IMPAX and ORYX systems from Douglas Instruments Ltd., UK, and theCyberlab 200, distributed by Gilson Inc., USA.

27 Apr 2015: Protein aggregateswere removed by a second spin at 200,000 g for 10 min. ... 200 pM) was allowed to bind for 10–15 min; this was followed by.

3 Dec 2004: interactions. J. Cell Biol. 155, 193–200 (2001). 20. Zhang, B. et al.