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15 Mar 2012: ParB (200 RU) was immobilized on an SPR sensor chip and allowed to react with wild-type MipZ protein (0.5 µM) that had been preincubated with 2 mM ATP and ... Subsequently, the protein was applied to a Sephacryl S-200 gel filtration column (GE Healthcare

17 Mar 2004: J. Cell Biol. 155,193-200.

29 May 2019: A) Cells expressing GFP-PrP and GFP-PrP were incubated on. ice with 200 nM extracellular Alexa647-conjugated Nb and Alexa647 fluorescence was measured by flow cytometry. ... pro. port. ion o. f cells. time (min). 0 50 100 150 200.

16 Jul 2023: STK strains were supplemented with 200 μM EdU(Thermo Fisher Scientific, C10639) for 10 min. ... Three microliters of resus-pended cells were applied to a freshly glow-discharged QuantifoilAu 200 mesh R2/2 or R2/1 grid.

15 Apr 2004: Calcu-lations employed torsional dynamics at 50 000 K for 1000steps of 15 ps, then a first slow cooling stage of torsionaldynamics (200 cycles each of 1000 steps of 15

11 Aug 2020: itotic. chro. mos. omes. Centrifugation 200 g. Supernatant containingchromosomes. Centrifugation 18,000 g(to pellet chromatin).

16 Aug 2004: Tris (pH 7.5),200 mM NaCl and 5 mM MgCl2 and eluted separately.Following calibration, 10 ml of ZapA protein at 10 mg/ml was injected and eluted as a ... 1 mM EDTA,1 mM NaN3 and 200 mM NaCl was placed in thesample compartment of an Epon double-sector

6 Mar 2001: For the continuousenzyme-coupled assay, purine nucleotide phosphorylase (20 U ml-1) and 2-amino-6-mercapto-7-methylpurine ribonucleoside (MESG) (200 mM) were also present in thereaction mix from the

23 Oct 2012: The image pixel size at this. magnification was 1.64 Å/pixel. In total of 200 CCD images were collected. ... µl were added to the 200 µl reaction mixture before initiating polymerization with 2 mM (final concentration).

5 Jul 2010: 3). Clearly visible are : (a) "rings" which have an insidediameter close to 800 A and an outer diameter the same as thatofthe pore complex itself(- 1,200 A) ; (b) large particles